Histamine, carbachol, and serotonin induce hyperresponsiveness to ATP in guinea pig tracheas: involvement of COX-2 pathway
Extracellular ATP promotes an indirect contraction of airway smooth muscle via the secondary release of thromboxane A 2 (TXA 2 ) from airway epithelium. Our aim was to evaluate if common contractile agonists modify this response to ATP. Tracheas from sensitized guinea pigs were used to evaluate ATP-...
Gespeichert in:
Veröffentlicht in: | Pflügers Archiv 2013-08, Vol.465 (8), p.1171-1179 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 1179 |
---|---|
container_issue | 8 |
container_start_page | 1171 |
container_title | Pflügers Archiv |
container_volume | 465 |
creator | Montaño, Luis M. Carbajal, Verónica Vargas, Mario H. García-Hernández, Luz M. Díaz-Hernández, Verónica Checa, Marco Barajas-López, Carlos |
description | Extracellular ATP promotes an indirect contraction of airway smooth muscle via the secondary release of thromboxane A
2
(TXA
2
) from airway epithelium. Our aim was to evaluate if common contractile agonists modify this response to ATP. Tracheas from sensitized guinea pigs were used to evaluate ATP-induced contractions before and after a transient contraction produced by histamine, carbachol, or serotonin. Epithelial mRNA for COX-1 and COX-2 was measured by RT-PCR and their expression assessed by immunohistochemistry. Compared with the initial response, ATP-induced contraction was potentiated by pretreatment with histamine, carbachol, or serotonin. Either suramin (antagonist of P2X and P2Y receptors) plus RB2 (antagonist of P2Y receptors) or indomethacin (inhibitor of COX-1 and COX-2) annulled the ATP-induced contraction, suggesting that it was mediated by P2Y receptor stimulation and TXA
2
production. When COX-2 was inhibited by SC-58125 or thromboxane receptors were antagonized by SQ-29548, just the potentiation was abolished, leaving the basal response intact. Airway epithelial cells showed increased COX-2 mRNA after stimulation with histamine or carbachol, but not serotonin, while COX-1 mRNA was unaffected. Immunochemistry corroborated this upregulation of COX-2. In conclusion, we showed for the first time that histamine and carbachol cause hyperresponsiveness to ATP by upregulating COX-2 in airway epithelium, which likely increases TXA
2
production. Serotonin-mediated hyperresponsiveness seems to be independent of COX-2 upregulation, but nonetheless is TXA
2
dependent. Because acetylcholine, histamine, and serotonin can be present during asthmatic exacerbations, their potential interactions with ATP might be relevant in its pathophysiology. |
doi_str_mv | 10.1007/s00424-013-1253-9 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1417403457</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3035998151</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-b184c66b464de94e4af98c2421fbc5705423339455a4e15eac3ba99f05f1918a3</originalsourceid><addsrcrecordid>eNp1kM9LHDEUgENpqavtH-ClBHo1mh8vMxNvsrRVEOxBwVvIZN_sjuwmYzKzZf3rjawWL54CL9_7HnyEHAt-KjivzzLnIIFxoZiQWjHzicwEKMlkGX0mM86VYFVdNQfkMOcHzrmERn4lB1JBrZXRM_J02efRbfqAJ9S71Dq_iusT6sKCZkxxjKEPtA-LySNd7QZMCfMQQ-63GDBnOkZ6cfu3EHQ5FYmjQ7-kYyoadPm8zLdxvcUNhpHGjs5v7pmkgxtX_9zuG_nSuXXG76_vEbn7_et2fsmub_5czS-umQfVjKwVDfiqaqGCBRpAcJ1pvAQputbrmmuQSikDWjtAodF51TpjOq47YUTj1BH5ufcOKT5OmEf7EKcUykkrQNTAFei6UGJP-RRzTtjZIfUbl3ZWcPtS2-5r25LWvtS2puz8eDVP7QYX_zfe8hZA7oFcvsIS07vTH1qfAXbRimM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1417403457</pqid></control><display><type>article</type><title>Histamine, carbachol, and serotonin induce hyperresponsiveness to ATP in guinea pig tracheas: involvement of COX-2 pathway</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Montaño, Luis M. ; Carbajal, Verónica ; Vargas, Mario H. ; García-Hernández, Luz M. ; Díaz-Hernández, Verónica ; Checa, Marco ; Barajas-López, Carlos</creator><creatorcontrib>Montaño, Luis M. ; Carbajal, Verónica ; Vargas, Mario H. ; García-Hernández, Luz M. ; Díaz-Hernández, Verónica ; Checa, Marco ; Barajas-López, Carlos</creatorcontrib><description>Extracellular ATP promotes an indirect contraction of airway smooth muscle via the secondary release of thromboxane A
2
(TXA
2
) from airway epithelium. Our aim was to evaluate if common contractile agonists modify this response to ATP. Tracheas from sensitized guinea pigs were used to evaluate ATP-induced contractions before and after a transient contraction produced by histamine, carbachol, or serotonin. Epithelial mRNA for COX-1 and COX-2 was measured by RT-PCR and their expression assessed by immunohistochemistry. Compared with the initial response, ATP-induced contraction was potentiated by pretreatment with histamine, carbachol, or serotonin. Either suramin (antagonist of P2X and P2Y receptors) plus RB2 (antagonist of P2Y receptors) or indomethacin (inhibitor of COX-1 and COX-2) annulled the ATP-induced contraction, suggesting that it was mediated by P2Y receptor stimulation and TXA
2
production. When COX-2 was inhibited by SC-58125 or thromboxane receptors were antagonized by SQ-29548, just the potentiation was abolished, leaving the basal response intact. Airway epithelial cells showed increased COX-2 mRNA after stimulation with histamine or carbachol, but not serotonin, while COX-1 mRNA was unaffected. Immunochemistry corroborated this upregulation of COX-2. In conclusion, we showed for the first time that histamine and carbachol cause hyperresponsiveness to ATP by upregulating COX-2 in airway epithelium, which likely increases TXA
2
production. Serotonin-mediated hyperresponsiveness seems to be independent of COX-2 upregulation, but nonetheless is TXA
2
dependent. Because acetylcholine, histamine, and serotonin can be present during asthmatic exacerbations, their potential interactions with ATP might be relevant in its pathophysiology.</description><identifier>ISSN: 0031-6768</identifier><identifier>EISSN: 1432-2013</identifier><identifier>DOI: 10.1007/s00424-013-1253-9</identifier><identifier>PMID: 23475395</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adenosine Triphosphate - metabolism ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Carbachol - pharmacology ; Cell Biology ; Cyclooxygenase 1 - genetics ; Cyclooxygenase 1 - metabolism ; Cyclooxygenase 2 - genetics ; Cyclooxygenase 2 - metabolism ; Cyclooxygenase 2 Inhibitors - pharmacology ; Cyclooxygenase Inhibitors - pharmacology ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Guinea Pigs ; Histamine - pharmacology ; Human Physiology ; Male ; Molecular and Cellular Mechanisms of Disease ; Molecular Medicine ; Muscle Contraction - drug effects ; Muscle Contraction - genetics ; Muscle, Smooth - drug effects ; Muscle, Smooth - metabolism ; Neurosciences ; Purinergic P2X Receptor Antagonists - pharmacology ; Purinergic P2Y Receptor Antagonists - pharmacology ; Receptors ; Receptors, Purinergic P2X - genetics ; Receptors, Purinergic P2X - metabolism ; Receptors, Purinergic P2Y - genetics ; Receptors, Purinergic P2Y - metabolism ; Respiratory Mucosa - drug effects ; Respiratory Mucosa - metabolism ; RNA, Messenger - genetics ; Serotonin - pharmacology ; Thromboxane A2 - genetics ; Thromboxane A2 - metabolism ; Trachea - drug effects ; Trachea - metabolism ; Up-Regulation - drug effects ; Up-Regulation - genetics</subject><ispartof>Pflügers Archiv, 2013-08, Vol.465 (8), p.1171-1179</ispartof><rights>Springer-Verlag Berlin Heidelberg 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-b184c66b464de94e4af98c2421fbc5705423339455a4e15eac3ba99f05f1918a3</citedby><cites>FETCH-LOGICAL-c438t-b184c66b464de94e4af98c2421fbc5705423339455a4e15eac3ba99f05f1918a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00424-013-1253-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00424-013-1253-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23475395$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Montaño, Luis M.</creatorcontrib><creatorcontrib>Carbajal, Verónica</creatorcontrib><creatorcontrib>Vargas, Mario H.</creatorcontrib><creatorcontrib>García-Hernández, Luz M.</creatorcontrib><creatorcontrib>Díaz-Hernández, Verónica</creatorcontrib><creatorcontrib>Checa, Marco</creatorcontrib><creatorcontrib>Barajas-López, Carlos</creatorcontrib><title>Histamine, carbachol, and serotonin induce hyperresponsiveness to ATP in guinea pig tracheas: involvement of COX-2 pathway</title><title>Pflügers Archiv</title><addtitle>Pflugers Arch - Eur J Physiol</addtitle><addtitle>Pflugers Arch</addtitle><description>Extracellular ATP promotes an indirect contraction of airway smooth muscle via the secondary release of thromboxane A
2
(TXA
2
) from airway epithelium. Our aim was to evaluate if common contractile agonists modify this response to ATP. Tracheas from sensitized guinea pigs were used to evaluate ATP-induced contractions before and after a transient contraction produced by histamine, carbachol, or serotonin. Epithelial mRNA for COX-1 and COX-2 was measured by RT-PCR and their expression assessed by immunohistochemistry. Compared with the initial response, ATP-induced contraction was potentiated by pretreatment with histamine, carbachol, or serotonin. Either suramin (antagonist of P2X and P2Y receptors) plus RB2 (antagonist of P2Y receptors) or indomethacin (inhibitor of COX-1 and COX-2) annulled the ATP-induced contraction, suggesting that it was mediated by P2Y receptor stimulation and TXA
2
production. When COX-2 was inhibited by SC-58125 or thromboxane receptors were antagonized by SQ-29548, just the potentiation was abolished, leaving the basal response intact. Airway epithelial cells showed increased COX-2 mRNA after stimulation with histamine or carbachol, but not serotonin, while COX-1 mRNA was unaffected. Immunochemistry corroborated this upregulation of COX-2. In conclusion, we showed for the first time that histamine and carbachol cause hyperresponsiveness to ATP by upregulating COX-2 in airway epithelium, which likely increases TXA
2
production. Serotonin-mediated hyperresponsiveness seems to be independent of COX-2 upregulation, but nonetheless is TXA
2
dependent. Because acetylcholine, histamine, and serotonin can be present during asthmatic exacerbations, their potential interactions with ATP might be relevant in its pathophysiology.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Carbachol - pharmacology</subject><subject>Cell Biology</subject><subject>Cyclooxygenase 1 - genetics</subject><subject>Cyclooxygenase 1 - metabolism</subject><subject>Cyclooxygenase 2 - genetics</subject><subject>Cyclooxygenase 2 - metabolism</subject><subject>Cyclooxygenase 2 Inhibitors - pharmacology</subject><subject>Cyclooxygenase Inhibitors - pharmacology</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Guinea Pigs</subject><subject>Histamine - pharmacology</subject><subject>Human Physiology</subject><subject>Male</subject><subject>Molecular and Cellular Mechanisms of Disease</subject><subject>Molecular Medicine</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle Contraction - genetics</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - metabolism</subject><subject>Neurosciences</subject><subject>Purinergic P2X Receptor Antagonists - pharmacology</subject><subject>Purinergic P2Y Receptor Antagonists - pharmacology</subject><subject>Receptors</subject><subject>Receptors, Purinergic P2X - genetics</subject><subject>Receptors, Purinergic P2X - metabolism</subject><subject>Receptors, Purinergic P2Y - genetics</subject><subject>Receptors, Purinergic P2Y - metabolism</subject><subject>Respiratory Mucosa - drug effects</subject><subject>Respiratory Mucosa - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>Serotonin - pharmacology</subject><subject>Thromboxane A2 - genetics</subject><subject>Thromboxane A2 - metabolism</subject><subject>Trachea - drug effects</subject><subject>Trachea - metabolism</subject><subject>Up-Regulation - drug effects</subject><subject>Up-Regulation - genetics</subject><issn>0031-6768</issn><issn>1432-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kM9LHDEUgENpqavtH-ClBHo1mh8vMxNvsrRVEOxBwVvIZN_sjuwmYzKzZf3rjawWL54CL9_7HnyEHAt-KjivzzLnIIFxoZiQWjHzicwEKMlkGX0mM86VYFVdNQfkMOcHzrmERn4lB1JBrZXRM_J02efRbfqAJ9S71Dq_iusT6sKCZkxxjKEPtA-LySNd7QZMCfMQQ-63GDBnOkZ6cfu3EHQ5FYmjQ7-kYyoadPm8zLdxvcUNhpHGjs5v7pmkgxtX_9zuG_nSuXXG76_vEbn7_et2fsmub_5czS-umQfVjKwVDfiqaqGCBRpAcJ1pvAQputbrmmuQSikDWjtAodF51TpjOq47YUTj1BH5ufcOKT5OmEf7EKcUykkrQNTAFei6UGJP-RRzTtjZIfUbl3ZWcPtS2-5r25LWvtS2puz8eDVP7QYX_zfe8hZA7oFcvsIS07vTH1qfAXbRimM</recordid><startdate>20130801</startdate><enddate>20130801</enddate><creator>Montaño, Luis M.</creator><creator>Carbajal, Verónica</creator><creator>Vargas, Mario H.</creator><creator>García-Hernández, Luz M.</creator><creator>Díaz-Hernández, Verónica</creator><creator>Checa, Marco</creator><creator>Barajas-López, Carlos</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20130801</creationdate><title>Histamine, carbachol, and serotonin induce hyperresponsiveness to ATP in guinea pig tracheas: involvement of COX-2 pathway</title><author>Montaño, Luis M. ; Carbajal, Verónica ; Vargas, Mario H. ; García-Hernández, Luz M. ; Díaz-Hernández, Verónica ; Checa, Marco ; Barajas-López, Carlos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-b184c66b464de94e4af98c2421fbc5705423339455a4e15eac3ba99f05f1918a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Carbachol - pharmacology</topic><topic>Cell Biology</topic><topic>Cyclooxygenase 1 - genetics</topic><topic>Cyclooxygenase 1 - metabolism</topic><topic>Cyclooxygenase 2 - genetics</topic><topic>Cyclooxygenase 2 - metabolism</topic><topic>Cyclooxygenase 2 Inhibitors - pharmacology</topic><topic>Cyclooxygenase Inhibitors - pharmacology</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Guinea Pigs</topic><topic>Histamine - pharmacology</topic><topic>Human Physiology</topic><topic>Male</topic><topic>Molecular and Cellular Mechanisms of Disease</topic><topic>Molecular Medicine</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle Contraction - genetics</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - metabolism</topic><topic>Neurosciences</topic><topic>Purinergic P2X Receptor Antagonists - pharmacology</topic><topic>Purinergic P2Y Receptor Antagonists - pharmacology</topic><topic>Receptors</topic><topic>Receptors, Purinergic P2X - genetics</topic><topic>Receptors, Purinergic P2X - metabolism</topic><topic>Receptors, Purinergic P2Y - genetics</topic><topic>Receptors, Purinergic P2Y - metabolism</topic><topic>Respiratory Mucosa - drug effects</topic><topic>Respiratory Mucosa - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>Serotonin - pharmacology</topic><topic>Thromboxane A2 - genetics</topic><topic>Thromboxane A2 - metabolism</topic><topic>Trachea - drug effects</topic><topic>Trachea - metabolism</topic><topic>Up-Regulation - drug effects</topic><topic>Up-Regulation - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Montaño, Luis M.</creatorcontrib><creatorcontrib>Carbajal, Verónica</creatorcontrib><creatorcontrib>Vargas, Mario H.</creatorcontrib><creatorcontrib>García-Hernández, Luz M.</creatorcontrib><creatorcontrib>Díaz-Hernández, Verónica</creatorcontrib><creatorcontrib>Checa, Marco</creatorcontrib><creatorcontrib>Barajas-López, Carlos</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Pflügers Archiv</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Montaño, Luis M.</au><au>Carbajal, Verónica</au><au>Vargas, Mario H.</au><au>García-Hernández, Luz M.</au><au>Díaz-Hernández, Verónica</au><au>Checa, Marco</au><au>Barajas-López, Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histamine, carbachol, and serotonin induce hyperresponsiveness to ATP in guinea pig tracheas: involvement of COX-2 pathway</atitle><jtitle>Pflügers Archiv</jtitle><stitle>Pflugers Arch - Eur J Physiol</stitle><addtitle>Pflugers Arch</addtitle><date>2013-08-01</date><risdate>2013</risdate><volume>465</volume><issue>8</issue><spage>1171</spage><epage>1179</epage><pages>1171-1179</pages><issn>0031-6768</issn><eissn>1432-2013</eissn><abstract>Extracellular ATP promotes an indirect contraction of airway smooth muscle via the secondary release of thromboxane A
2
(TXA
2
) from airway epithelium. Our aim was to evaluate if common contractile agonists modify this response to ATP. Tracheas from sensitized guinea pigs were used to evaluate ATP-induced contractions before and after a transient contraction produced by histamine, carbachol, or serotonin. Epithelial mRNA for COX-1 and COX-2 was measured by RT-PCR and their expression assessed by immunohistochemistry. Compared with the initial response, ATP-induced contraction was potentiated by pretreatment with histamine, carbachol, or serotonin. Either suramin (antagonist of P2X and P2Y receptors) plus RB2 (antagonist of P2Y receptors) or indomethacin (inhibitor of COX-1 and COX-2) annulled the ATP-induced contraction, suggesting that it was mediated by P2Y receptor stimulation and TXA
2
production. When COX-2 was inhibited by SC-58125 or thromboxane receptors were antagonized by SQ-29548, just the potentiation was abolished, leaving the basal response intact. Airway epithelial cells showed increased COX-2 mRNA after stimulation with histamine or carbachol, but not serotonin, while COX-1 mRNA was unaffected. Immunochemistry corroborated this upregulation of COX-2. In conclusion, we showed for the first time that histamine and carbachol cause hyperresponsiveness to ATP by upregulating COX-2 in airway epithelium, which likely increases TXA
2
production. Serotonin-mediated hyperresponsiveness seems to be independent of COX-2 upregulation, but nonetheless is TXA
2
dependent. Because acetylcholine, histamine, and serotonin can be present during asthmatic exacerbations, their potential interactions with ATP might be relevant in its pathophysiology.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>23475395</pmid><doi>10.1007/s00424-013-1253-9</doi><tpages>9</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0031-6768 |
ispartof | Pflügers Archiv, 2013-08, Vol.465 (8), p.1171-1179 |
issn | 0031-6768 1432-2013 |
language | eng |
recordid | cdi_proquest_journals_1417403457 |
source | MEDLINE; SpringerLink Journals |
subjects | Adenosine Triphosphate - metabolism Animals Biomedical and Life Sciences Biomedicine Carbachol - pharmacology Cell Biology Cyclooxygenase 1 - genetics Cyclooxygenase 1 - metabolism Cyclooxygenase 2 - genetics Cyclooxygenase 2 - metabolism Cyclooxygenase 2 Inhibitors - pharmacology Cyclooxygenase Inhibitors - pharmacology Epithelial Cells - drug effects Epithelial Cells - metabolism Guinea Pigs Histamine - pharmacology Human Physiology Male Molecular and Cellular Mechanisms of Disease Molecular Medicine Muscle Contraction - drug effects Muscle Contraction - genetics Muscle, Smooth - drug effects Muscle, Smooth - metabolism Neurosciences Purinergic P2X Receptor Antagonists - pharmacology Purinergic P2Y Receptor Antagonists - pharmacology Receptors Receptors, Purinergic P2X - genetics Receptors, Purinergic P2X - metabolism Receptors, Purinergic P2Y - genetics Receptors, Purinergic P2Y - metabolism Respiratory Mucosa - drug effects Respiratory Mucosa - metabolism RNA, Messenger - genetics Serotonin - pharmacology Thromboxane A2 - genetics Thromboxane A2 - metabolism Trachea - drug effects Trachea - metabolism Up-Regulation - drug effects Up-Regulation - genetics |
title | Histamine, carbachol, and serotonin induce hyperresponsiveness to ATP in guinea pig tracheas: involvement of COX-2 pathway |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T14%3A37%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Histamine,%20carbachol,%20and%20serotonin%20induce%20hyperresponsiveness%20to%20ATP%20in%20guinea%20pig%20tracheas:%20involvement%20of%20COX-2%20pathway&rft.jtitle=Pfl%C3%BCgers%20Archiv&rft.au=Monta%C3%B1o,%20Luis%20M.&rft.date=2013-08-01&rft.volume=465&rft.issue=8&rft.spage=1171&rft.epage=1179&rft.pages=1171-1179&rft.issn=0031-6768&rft.eissn=1432-2013&rft_id=info:doi/10.1007/s00424-013-1253-9&rft_dat=%3Cproquest_cross%3E3035998151%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1417403457&rft_id=info:pmid/23475395&rfr_iscdi=true |