Mechanism and kinetics of a sodium-driven bacterial flagellar motor
The bacterial flagellar motor is a large rotary molecular machine that propels swimming bacteria, powered by a transmembrane electrochemical potential difference. It consists of an ∼50-nm rotor and up to ∼10 independent stators anchored to the cell wall. We measured torque–speed relationships of sin...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2013-07, Vol.110 (28), p.E2544-E2551 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Lo, Chien-Jung Sowa, Yoshiyuki Pilizota, Teuta Berry, Richard M |
| description | The bacterial flagellar motor is a large rotary molecular machine that propels swimming bacteria, powered by a transmembrane electrochemical potential difference. It consists of an ∼50-nm rotor and up to ∼10 independent stators anchored to the cell wall. We measured torque–speed relationships of single-stator motors under 25 different combinations of electrical and chemical potential. All 25 torque–speed curves had the same concave-down shape as fully energized wild-type motors, and each stator passes at least 37 ± 2 ions per revolution. We used the results to explore the 25-dimensional parameter space of generalized kinetic models for the motor mechanism, finding 830 parameter sets consistent with the data. Analysis of these sets showed that the motor mechanism has a “powerstroke” in either ion binding or transit; ion transit is channel-like rather than carrier-like; and the rate-limiting step in the motor cycle is ion binding at low concentration, ion transit, or release at high concentration. |
| doi_str_mv | 10.1073/pnas.1301664110 |
| format | Article |
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It consists of an ∼50-nm rotor and up to ∼10 independent stators anchored to the cell wall. We measured torque–speed relationships of single-stator motors under 25 different combinations of electrical and chemical potential. All 25 torque–speed curves had the same concave-down shape as fully energized wild-type motors, and each stator passes at least 37 ± 2 ions per revolution. We used the results to explore the 25-dimensional parameter space of generalized kinetic models for the motor mechanism, finding 830 parameter sets consistent with the data. Analysis of these sets showed that the motor mechanism has a “powerstroke” in either ion binding or transit; ion transit is channel-like rather than carrier-like; and the rate-limiting step in the motor cycle is ion binding at low concentration, ion transit, or release at high concentration.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1301664110</identifier><identifier>PMID: 23788659</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Bacteria ; bacterial motility ; Bacterial Physiological Phenomena ; Biological Sciences ; cell walls ; electrochemistry ; Flagella - physiology ; Ions ; Kinetics ; Membranes ; Molecules ; PNAS Plus ; Sodium - physiology</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2013-07, Vol.110 (28), p.E2544-E2551</ispartof><rights>Copyright National Academy of Sciences Jul 9, 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c569t-9fcd8c5679abcef33ad1c4ff2240d56c2d5dca38e13469b48cd3d7b93f9d215f3</citedby><cites>FETCH-LOGICAL-c569t-9fcd8c5679abcef33ad1c4ff2240d56c2d5dca38e13469b48cd3d7b93f9d215f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/110/28.cover.gif</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710797/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710797/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23788659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lo, Chien-Jung</creatorcontrib><creatorcontrib>Sowa, Yoshiyuki</creatorcontrib><creatorcontrib>Pilizota, Teuta</creatorcontrib><creatorcontrib>Berry, Richard M</creatorcontrib><title>Mechanism and kinetics of a sodium-driven bacterial flagellar motor</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The bacterial flagellar motor is a large rotary molecular machine that propels swimming bacteria, powered by a transmembrane electrochemical potential difference. 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Analysis of these sets showed that the motor mechanism has a “powerstroke” in either ion binding or transit; ion transit is channel-like rather than carrier-like; and the rate-limiting step in the motor cycle is ion binding at low concentration, ion transit, or release at high concentration.</description><subject>Bacteria</subject><subject>bacterial motility</subject><subject>Bacterial Physiological Phenomena</subject><subject>Biological Sciences</subject><subject>cell walls</subject><subject>electrochemistry</subject><subject>Flagella - physiology</subject><subject>Ions</subject><subject>Kinetics</subject><subject>Membranes</subject><subject>Molecules</subject><subject>PNAS Plus</subject><subject>Sodium - physiology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctv1DAQxi0EokvhzA0iceGSdvyMfUFCq_KQijhAz5bjx9YliRc7qcR_j6NdlseFk0fyb76Z-T6EnmO4wNDRy_1kygWmgIVgGMMDtMGgcCuYgodoA0C6VjLCztCTUu4AQHEJj9EZoZ2UgqsN2n7y9tZMsYyNmVzzLU5-jrY0KTSmKcnFZWxdjvd-anpjZ5-jGZowmJ0fBpObMc0pP0WPghmKf3Z8z9HNu6uv2w_t9ef3H7dvr1vLhZpbFayTteyU6a0PlBqHLQuBEAaOC0scd9ZQ6TFlQvVMWkdd1ysalCOYB3qO3hx090s_emf9NGcz6H2Oo8k_dDJR__0zxVu9S_eadtUs1VWB10eBnL4vvsx6jMWul0w-LUVjCbT6Jyj9P0qV4tAJsaKv_kHv0pKn6oTGDLBijBNVqcsDZXMqJftw2huDXrPUa5b6d5a148Wf5574X-FVoDkCa-dJruoRqa8IZ6wiLw9IMEmbXY5F33whdQQAphIEpj8BwRCufQ</recordid><startdate>20130709</startdate><enddate>20130709</enddate><creator>Lo, Chien-Jung</creator><creator>Sowa, Yoshiyuki</creator><creator>Pilizota, Teuta</creator><creator>Berry, Richard M</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20130709</creationdate><title>Mechanism and kinetics of a sodium-driven bacterial flagellar motor</title><author>Lo, Chien-Jung ; 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| subjects | Bacteria bacterial motility Bacterial Physiological Phenomena Biological Sciences cell walls electrochemistry Flagella - physiology Ions Kinetics Membranes Molecules PNAS Plus Sodium - physiology |
| title | Mechanism and kinetics of a sodium-driven bacterial flagellar motor |
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