RT-PCR evaluation of foot and mouth disease serotype O in saliva, tracheal and vesicular samples of goats in Punjab, Pakistan
The aim of present study was to evaluate the RT-PCR technique in the original field samples from goat for the diagnosis of foot and mouth disease (FMD) within 24 hours. A total of 280 samples were collected from thirty herds of goats in twelve different outbreaks clinically suspected for FMD near ou...
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| Veröffentlicht in: | Pakistan journal of zoology 2011-08, Vol.43 (4), p.808-810 |
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| creator | Saeed, A. (Bahauddin Zakariya Univ., Multan (Pakistan). Inst. of Bio-Technology) Waheed, U. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Coll. of Veterinary and Animal Sciences, Jhang Sub-campus) Arshad, M. (National Inst. for Biotechnology and Genetic Engineering, Faisalabad (Pakistan)) Khan, Q.M Ali, M Babar, M |
| description | The aim of present study was to evaluate the RT-PCR technique in the original field samples from goat for the diagnosis of foot and mouth disease (FMD) within 24 hours. A total of 280 samples were collected from thirty herds of goats in twelve different outbreaks clinically suspected for FMD near outbreak epicenters of other livestock and evaluated by RT-PCR with 171 positive results. Universal primer pair P1/P2 detected 30% and 1F/1R detected 61% from total collected samples. AU(O)/AU(rev) and AU(O)/PNK61 primer pairs confirmed O serotype of FMD in 90.6% and 74.4% respectively out of 43 representative samples, whilst O-1C(ARS4)/PNK61 O primer pairs detected only 18.6% samples. The VP1gene sequence analysis revealed O serotype of FMD PanAsial lineage. The maximum samples can be detected by 1F/1R, AU(O)/AU(rev) and AU(O)/PNK61 primer pairs; whilst P1/P2, AU(O)/AU(rev) and ARS4/NK61 can be a better combination for the sequence analysis from original field materials of goats. |
| format | Article |
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(Bahauddin Zakariya Univ., Multan (Pakistan). Inst. of Bio-Technology) ; Waheed, U. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Coll. of Veterinary and Animal Sciences, Jhang Sub-campus) ; Arshad, M. (National Inst. for Biotechnology and Genetic Engineering, Faisalabad (Pakistan)) ; Khan, Q.M ; Ali, M ; Babar, M</creator><creatorcontrib>Saeed, A. (Bahauddin Zakariya Univ., Multan (Pakistan). Inst. of Bio-Technology) ; Waheed, U. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Coll. of Veterinary and Animal Sciences, Jhang Sub-campus) ; Arshad, M. (National Inst. for Biotechnology and Genetic Engineering, Faisalabad (Pakistan)) ; Khan, Q.M ; Ali, M ; Babar, M</creatorcontrib><description>The aim of present study was to evaluate the RT-PCR technique in the original field samples from goat for the diagnosis of foot and mouth disease (FMD) within 24 hours. A total of 280 samples were collected from thirty herds of goats in twelve different outbreaks clinically suspected for FMD near outbreak epicenters of other livestock and evaluated by RT-PCR with 171 positive results. Universal primer pair P1/P2 detected 30% and 1F/1R detected 61% from total collected samples. AU(O)/AU(rev) and AU(O)/PNK61 primer pairs confirmed O serotype of FMD in 90.6% and 74.4% respectively out of 43 representative samples, whilst O-1C(ARS4)/PNK61 O primer pairs detected only 18.6% samples. The VP1gene sequence analysis revealed O serotype of FMD PanAsial lineage. The maximum samples can be detected by 1F/1R, AU(O)/AU(rev) and AU(O)/PNK61 primer pairs; whilst P1/P2, AU(O)/AU(rev) and ARS4/NK61 can be a better combination for the sequence analysis from original field materials of goats.</description><identifier>ISSN: 0030-9923</identifier><language>eng</language><publisher>Lahore: Knowledge Bylanes</publisher><subject>Analysis ; Diagnosis ; EVALUATION ; FOOT AND MOUTH DISEASE ; GOATS ; Health aspects ; Livestock ; PAKISTAN ; PCR ; SALVIA ; SEROTYPES ; TRACHEA ; VESICULAR GLANDS</subject><ispartof>Pakistan journal of zoology, 2011-08, Vol.43 (4), p.808-810</ispartof><rights>COPYRIGHT 2011 Knowledge Bylanes</rights><rights>(c) 2011 Pakistan Journal of Zoology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Saeed, A. (Bahauddin Zakariya Univ., Multan (Pakistan). Inst. of Bio-Technology)</creatorcontrib><creatorcontrib>Waheed, U. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Coll. of Veterinary and Animal Sciences, Jhang Sub-campus)</creatorcontrib><creatorcontrib>Arshad, M. (National Inst. for Biotechnology and Genetic Engineering, Faisalabad (Pakistan))</creatorcontrib><creatorcontrib>Khan, Q.M</creatorcontrib><creatorcontrib>Ali, M</creatorcontrib><creatorcontrib>Babar, M</creatorcontrib><title>RT-PCR evaluation of foot and mouth disease serotype O in saliva, tracheal and vesicular samples of goats in Punjab, Pakistan</title><title>Pakistan journal of zoology</title><description>The aim of present study was to evaluate the RT-PCR technique in the original field samples from goat for the diagnosis of foot and mouth disease (FMD) within 24 hours. A total of 280 samples were collected from thirty herds of goats in twelve different outbreaks clinically suspected for FMD near outbreak epicenters of other livestock and evaluated by RT-PCR with 171 positive results. Universal primer pair P1/P2 detected 30% and 1F/1R detected 61% from total collected samples. AU(O)/AU(rev) and AU(O)/PNK61 primer pairs confirmed O serotype of FMD in 90.6% and 74.4% respectively out of 43 representative samples, whilst O-1C(ARS4)/PNK61 O primer pairs detected only 18.6% samples. The VP1gene sequence analysis revealed O serotype of FMD PanAsial lineage. The maximum samples can be detected by 1F/1R, AU(O)/AU(rev) and AU(O)/PNK61 primer pairs; whilst P1/P2, AU(O)/AU(rev) and ARS4/NK61 can be a better combination for the sequence analysis from original field materials of goats.</description><subject>Analysis</subject><subject>Diagnosis</subject><subject>EVALUATION</subject><subject>FOOT AND MOUTH DISEASE</subject><subject>GOATS</subject><subject>Health aspects</subject><subject>Livestock</subject><subject>PAKISTAN</subject><subject>PCR</subject><subject>SALVIA</subject><subject>SEROTYPES</subject><subject>TRACHEA</subject><subject>VESICULAR GLANDS</subject><issn>0030-9923</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNotkE1rwzAMhnPYYGXrTxgYdm2GP2InOZayL1ZoKd05qLHcukvtLnYKO-y_z10nHQTS-7xCuspGlAqa1zUXN9k4hD1NUSjJeTXKflbrfDlbETxBN0C03hFviPE-EnCaHPwQd0TbgBCQBOx9_D4iWRDrSIDOnmBCYg_tDqH7A04YbDt00Kfx4dhhONttPcRwRpaD28NmQpbwaUMEd5ddG-gCjv_rbfbx_LSevebzxcvbbDrPDS-KmGtRKcXAyEqVXLJNWzGlqZC6FEWhDReolNxQoFQxUVdQFqouW1kZoxGNNOI2e7j4Hnv_NWCIzd4PvUsrGyZKWhdlKWhSPV5UW-iwsc7482kpNR5s6x0am_pTriRjUgqVgPsLYMA3sO1taJbvnDKeHqyS4hdOhHIt</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>Saeed, A. 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(Bahauddin Zakariya Univ., Multan (Pakistan). Inst. of Bio-Technology) ; Waheed, U. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Coll. of Veterinary and Animal Sciences, Jhang Sub-campus) ; Arshad, M. 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Coll. of Veterinary and Animal Sciences, Jhang Sub-campus)</creatorcontrib><creatorcontrib>Arshad, M. 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(Bahauddin Zakariya Univ., Multan (Pakistan). Inst. of Bio-Technology)</au><au>Waheed, U. (University of Veterinary and Animal Sciences, Lahore (Pakistan). Coll. of Veterinary and Animal Sciences, Jhang Sub-campus)</au><au>Arshad, M. (National Inst. for Biotechnology and Genetic Engineering, Faisalabad (Pakistan))</au><au>Khan, Q.M</au><au>Ali, M</au><au>Babar, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RT-PCR evaluation of foot and mouth disease serotype O in saliva, tracheal and vesicular samples of goats in Punjab, Pakistan</atitle><jtitle>Pakistan journal of zoology</jtitle><date>2011-08-01</date><risdate>2011</risdate><volume>43</volume><issue>4</issue><spage>808</spage><epage>810</epage><pages>808-810</pages><issn>0030-9923</issn><abstract>The aim of present study was to evaluate the RT-PCR technique in the original field samples from goat for the diagnosis of foot and mouth disease (FMD) within 24 hours. A total of 280 samples were collected from thirty herds of goats in twelve different outbreaks clinically suspected for FMD near outbreak epicenters of other livestock and evaluated by RT-PCR with 171 positive results. Universal primer pair P1/P2 detected 30% and 1F/1R detected 61% from total collected samples. AU(O)/AU(rev) and AU(O)/PNK61 primer pairs confirmed O serotype of FMD in 90.6% and 74.4% respectively out of 43 representative samples, whilst O-1C(ARS4)/PNK61 O primer pairs detected only 18.6% samples. The VP1gene sequence analysis revealed O serotype of FMD PanAsial lineage. The maximum samples can be detected by 1F/1R, AU(O)/AU(rev) and AU(O)/PNK61 primer pairs; whilst P1/P2, AU(O)/AU(rev) and ARS4/NK61 can be a better combination for the sequence analysis from original field materials of goats.</abstract><cop>Lahore</cop><pub>Knowledge Bylanes</pub><tpages>3</tpages></addata></record> |
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| subjects | Analysis Diagnosis EVALUATION FOOT AND MOUTH DISEASE GOATS Health aspects Livestock PAKISTAN PCR SALVIA SEROTYPES TRACHEA VESICULAR GLANDS |
| title | RT-PCR evaluation of foot and mouth disease serotype O in saliva, tracheal and vesicular samples of goats in Punjab, Pakistan |
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