The development of an indirect ELISA for the detection of antibodies to goose parvovirus in blood serum
This work aimed to develop an indirect enzyme‐linked immunosorbent assay (i‐ELISA), based on the structural protein VP2 of goose parvovirus (GPV), for the detection of antibodies to GPV in blood serum. In this study, the capsid protein VP2 of GPV was expressed in Escherichia coli and purified by nic...
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| Veröffentlicht in: | Letters in applied microbiology 2013-07, Vol.57 (1), p.26-32 |
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| creator | Fan, J.‐H. Zuo, Y.‐Z. Yang, Z. Pei, L.‐H. |
| description | This work aimed to develop an indirect enzyme‐linked immunosorbent assay (i‐ELISA), based on the structural protein VP2 of goose parvovirus (GPV), for the detection of antibodies to GPV in blood serum. In this study, the capsid protein VP2 of GPV was expressed in Escherichia coli and purified by nickel chromatography. The purified protein was capable of reacting with GPV antibodies, as analysed by Western blot. An i‐ELISA based on the protein VP2 was developed. For evaluation of the newly developed ELISA, 192 field sera collected from six goose herds were tested in parallel by the serum neutralization (SN) test. The result showed that 138 samples were positive and 49 negative in both cases. The agreement of the two tests was 97·3%. This i‐ELISA has high sensitivity and good specificity and is an alternative serologic test for the detection of the antibodies to GPV in blood serum.
Significance and Impact of the Study
In this study, we expressed and purified the capsid protein VP2 of GPV and developed a VP2‐based i‐ELISA for the detection of GPV antibodies. This i‐ELISA can be used for evaluating the immune effects of GPV vaccine and for diagnosing of GPV infection.
Significance and Impact of the Study: In this study, we expressed and purified the capsid protein VP2 of GPV and developed a VP2‐based i‐ELISA for the detection of GPV antibodies. This i‐ELISA can be used for evaluating the immune effects of GPV vaccine and for diagnosing of GPV infection. |
| doi_str_mv | 10.1111/lam.12070 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1366667877</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2993850181</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3850-6eed38381fb6107b7084d0dd8e7edb4803832ba296cfab33d957e29175d07c773</originalsourceid><addsrcrecordid>eNp10E9LwzAYBvAgipvTg19AAuLBQ12S_kl6HGPqYOLBCd5K2rydGW0zk3ayb29cp558L4G8P94HHoQuKbmjfsaVrO8oI5wcoSGNOAt4Er8doyFhSRIIFkcDdObcmhAiKEtP0YCFMSWMJ0O0Wr4DVrCFymxqaFpsSiwbrBulLRQtni3mLxNcGovbPWz9pzZNz1qdG6XB4dbglTEO8Ebardlq2zl_AueVMQo7sF19jk5KWTm4OLwj9Ho_W04fg8Xzw3w6WQRFKGISJAAqFKGgZZ5QwnNORKSIUgI4qDwSxC9ZLlmaFKXMw1ClMQeWUh4rwgvOwxG67u9urPnowLXZ2nS28ZEZDRM_XOzVba8Ka5yzUGYbq2tpdxkl2Xelma8021fq7dXhYpfXoH7lT4ce3ByAdIWsSiubQrs_x6NYEJF6N-7dp65g939itpg89dFfRrOMXQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1366667877</pqid></control><display><type>article</type><title>The development of an indirect ELISA for the detection of antibodies to goose parvovirus in blood serum</title><source>MEDLINE</source><source>Wiley Online Library</source><source>Oxford Academic Journals (OUP)</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>Fan, J.‐H. ; Zuo, Y.‐Z. ; Yang, Z. ; Pei, L.‐H.</creator><creatorcontrib>Fan, J.‐H. ; Zuo, Y.‐Z. ; Yang, Z. ; Pei, L.‐H.</creatorcontrib><description>This work aimed to develop an indirect enzyme‐linked immunosorbent assay (i‐ELISA), based on the structural protein VP2 of goose parvovirus (GPV), for the detection of antibodies to GPV in blood serum. In this study, the capsid protein VP2 of GPV was expressed in Escherichia coli and purified by nickel chromatography. The purified protein was capable of reacting with GPV antibodies, as analysed by Western blot. An i‐ELISA based on the protein VP2 was developed. For evaluation of the newly developed ELISA, 192 field sera collected from six goose herds were tested in parallel by the serum neutralization (SN) test. The result showed that 138 samples were positive and 49 negative in both cases. The agreement of the two tests was 97·3%. This i‐ELISA has high sensitivity and good specificity and is an alternative serologic test for the detection of the antibodies to GPV in blood serum.
Significance and Impact of the Study
In this study, we expressed and purified the capsid protein VP2 of GPV and developed a VP2‐based i‐ELISA for the detection of GPV antibodies. This i‐ELISA can be used for evaluating the immune effects of GPV vaccine and for diagnosing of GPV infection.
Significance and Impact of the Study: In this study, we expressed and purified the capsid protein VP2 of GPV and developed a VP2‐based i‐ELISA for the detection of GPV antibodies. This i‐ELISA can be used for evaluating the immune effects of GPV vaccine and for diagnosing of GPV infection.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/lam.12070</identifier><identifier>PMID: 23510276</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Animals ; Antibodies, Viral - blood ; Antibodies, Viral - isolation & purification ; Biological and medical sciences ; Capsid Proteins ; ELISA ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - veterinary ; Fundamental and applied biological sciences. Psychology ; Geese ; goose parvovirus ; Microbiology ; Parvoviridae Infections - diagnosis ; Parvoviridae Infections - veterinary ; Parvoviridae Infections - virology ; Parvovirus - isolation & purification ; Poultry Diseases - diagnosis ; Poultry Diseases - virology ; Sensitivity and Specificity ; Serologic Tests - methods ; serum antibodies ; VP2 protein</subject><ispartof>Letters in applied microbiology, 2013-07, Vol.57 (1), p.26-32</ispartof><rights>2013 The Society for Applied Microbiology</rights><rights>2014 INIST-CNRS</rights><rights>2013 The Society for Applied Microbiology.</rights><rights>Copyright © 2013 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3850-6eed38381fb6107b7084d0dd8e7edb4803832ba296cfab33d957e29175d07c773</citedby><cites>FETCH-LOGICAL-c3850-6eed38381fb6107b7084d0dd8e7edb4803832ba296cfab33d957e29175d07c773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Flam.12070$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Flam.12070$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27458089$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23510276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fan, J.‐H.</creatorcontrib><creatorcontrib>Zuo, Y.‐Z.</creatorcontrib><creatorcontrib>Yang, Z.</creatorcontrib><creatorcontrib>Pei, L.‐H.</creatorcontrib><title>The development of an indirect ELISA for the detection of antibodies to goose parvovirus in blood serum</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>This work aimed to develop an indirect enzyme‐linked immunosorbent assay (i‐ELISA), based on the structural protein VP2 of goose parvovirus (GPV), for the detection of antibodies to GPV in blood serum. In this study, the capsid protein VP2 of GPV was expressed in Escherichia coli and purified by nickel chromatography. The purified protein was capable of reacting with GPV antibodies, as analysed by Western blot. An i‐ELISA based on the protein VP2 was developed. For evaluation of the newly developed ELISA, 192 field sera collected from six goose herds were tested in parallel by the serum neutralization (SN) test. The result showed that 138 samples were positive and 49 negative in both cases. The agreement of the two tests was 97·3%. This i‐ELISA has high sensitivity and good specificity and is an alternative serologic test for the detection of the antibodies to GPV in blood serum.
Significance and Impact of the Study
In this study, we expressed and purified the capsid protein VP2 of GPV and developed a VP2‐based i‐ELISA for the detection of GPV antibodies. This i‐ELISA can be used for evaluating the immune effects of GPV vaccine and for diagnosing of GPV infection.
Significance and Impact of the Study: In this study, we expressed and purified the capsid protein VP2 of GPV and developed a VP2‐based i‐ELISA for the detection of GPV antibodies. This i‐ELISA can be used for evaluating the immune effects of GPV vaccine and for diagnosing of GPV infection.</description><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Antibodies, Viral - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Capsid Proteins</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Geese</subject><subject>goose parvovirus</subject><subject>Microbiology</subject><subject>Parvoviridae Infections - diagnosis</subject><subject>Parvoviridae Infections - veterinary</subject><subject>Parvoviridae Infections - virology</subject><subject>Parvovirus - isolation & purification</subject><subject>Poultry Diseases - diagnosis</subject><subject>Poultry Diseases - virology</subject><subject>Sensitivity and Specificity</subject><subject>Serologic Tests - methods</subject><subject>serum antibodies</subject><subject>VP2 protein</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10E9LwzAYBvAgipvTg19AAuLBQ12S_kl6HGPqYOLBCd5K2rydGW0zk3ayb29cp558L4G8P94HHoQuKbmjfsaVrO8oI5wcoSGNOAt4Er8doyFhSRIIFkcDdObcmhAiKEtP0YCFMSWMJ0O0Wr4DVrCFymxqaFpsSiwbrBulLRQtni3mLxNcGovbPWz9pzZNz1qdG6XB4dbglTEO8Ebardlq2zl_AueVMQo7sF19jk5KWTm4OLwj9Ho_W04fg8Xzw3w6WQRFKGISJAAqFKGgZZ5QwnNORKSIUgI4qDwSxC9ZLlmaFKXMw1ClMQeWUh4rwgvOwxG67u9urPnowLXZ2nS28ZEZDRM_XOzVba8Ka5yzUGYbq2tpdxkl2Xelma8021fq7dXhYpfXoH7lT4ce3ByAdIWsSiubQrs_x6NYEJF6N-7dp65g939itpg89dFfRrOMXQ</recordid><startdate>201307</startdate><enddate>201307</enddate><creator>Fan, J.‐H.</creator><creator>Zuo, Y.‐Z.</creator><creator>Yang, Z.</creator><creator>Pei, L.‐H.</creator><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>201307</creationdate><title>The development of an indirect ELISA for the detection of antibodies to goose parvovirus in blood serum</title><author>Fan, J.‐H. ; Zuo, Y.‐Z. ; Yang, Z. ; Pei, L.‐H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3850-6eed38381fb6107b7084d0dd8e7edb4803832ba296cfab33d957e29175d07c773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Antibodies, Viral - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Capsid Proteins</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Geese</topic><topic>goose parvovirus</topic><topic>Microbiology</topic><topic>Parvoviridae Infections - diagnosis</topic><topic>Parvoviridae Infections - veterinary</topic><topic>Parvoviridae Infections - virology</topic><topic>Parvovirus - isolation & purification</topic><topic>Poultry Diseases - diagnosis</topic><topic>Poultry Diseases - virology</topic><topic>Sensitivity and Specificity</topic><topic>Serologic Tests - methods</topic><topic>serum antibodies</topic><topic>VP2 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fan, J.‐H.</creatorcontrib><creatorcontrib>Zuo, Y.‐Z.</creatorcontrib><creatorcontrib>Yang, Z.</creatorcontrib><creatorcontrib>Pei, L.‐H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fan, J.‐H.</au><au>Zuo, Y.‐Z.</au><au>Yang, Z.</au><au>Pei, L.‐H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The development of an indirect ELISA for the detection of antibodies to goose parvovirus in blood serum</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2013-07</date><risdate>2013</risdate><volume>57</volume><issue>1</issue><spage>26</spage><epage>32</epage><pages>26-32</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><coden>LAMIE7</coden><abstract>This work aimed to develop an indirect enzyme‐linked immunosorbent assay (i‐ELISA), based on the structural protein VP2 of goose parvovirus (GPV), for the detection of antibodies to GPV in blood serum. In this study, the capsid protein VP2 of GPV was expressed in Escherichia coli and purified by nickel chromatography. The purified protein was capable of reacting with GPV antibodies, as analysed by Western blot. An i‐ELISA based on the protein VP2 was developed. For evaluation of the newly developed ELISA, 192 field sera collected from six goose herds were tested in parallel by the serum neutralization (SN) test. The result showed that 138 samples were positive and 49 negative in both cases. The agreement of the two tests was 97·3%. This i‐ELISA has high sensitivity and good specificity and is an alternative serologic test for the detection of the antibodies to GPV in blood serum.
Significance and Impact of the Study
In this study, we expressed and purified the capsid protein VP2 of GPV and developed a VP2‐based i‐ELISA for the detection of GPV antibodies. This i‐ELISA can be used for evaluating the immune effects of GPV vaccine and for diagnosing of GPV infection.
Significance and Impact of the Study: In this study, we expressed and purified the capsid protein VP2 of GPV and developed a VP2‐based i‐ELISA for the detection of GPV antibodies. This i‐ELISA can be used for evaluating the immune effects of GPV vaccine and for diagnosing of GPV infection.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>23510276</pmid><doi>10.1111/lam.12070</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Letters in applied microbiology, 2013-07, Vol.57 (1), p.26-32 |
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| source | MEDLINE; Wiley Online Library; Oxford Academic Journals (OUP); Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Animals Antibodies, Viral - blood Antibodies, Viral - isolation & purification Biological and medical sciences Capsid Proteins ELISA Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Fundamental and applied biological sciences. Psychology Geese goose parvovirus Microbiology Parvoviridae Infections - diagnosis Parvoviridae Infections - veterinary Parvoviridae Infections - virology Parvovirus - isolation & purification Poultry Diseases - diagnosis Poultry Diseases - virology Sensitivity and Specificity Serologic Tests - methods serum antibodies VP2 protein |
| title | The development of an indirect ELISA for the detection of antibodies to goose parvovirus in blood serum |
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