Relationship of DNA degradation by Saccharomyces cerevisiae Exonuclease 1 and its stimulation by RPA and Mre11-Rad50-Xrs2 to DNA end resection
Significance The repair of double-strand DNA breaks by homologous recombination is initiated by the nucleolytic resection of the 5′-terminated strand at the DNA break. Genetic work in Saccharomyces cerevisiae identified three DNA end resection pathways: Mre11-Rad50-Xrs2, Dna2-Sgs1-Top3-Rmi1, and Exo...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2013-04, Vol.110 (18), p.E1661-E1668 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Cannavo, Elda Cejka, Petr Kowalczykowski, Stephen C |
| description | Significance The repair of double-strand DNA breaks by homologous recombination is initiated by the nucleolytic resection of the 5′-terminated strand at the DNA break. Genetic work in Saccharomyces cerevisiae identified three DNA end resection pathways: Mre11-Rad50-Xrs2, Dna2-Sgs1-Top3-Rmi1, and Exo1. Here we investigated the relationship between the three nucleolytic complexes in vitro. With a focus on Exo1, we show that it is stimulated by the single-strand DNA-binding protein, RPA, and also by the Mre11-Rad50-Xrs2 complex. Furthermore, our analysis provides biochemical evidence for the view that Exo1 and Dna2-Sgs1-Top3-Rmi1 function downstream of Mre11-Rad50-Xrs2 as independent and mutually exclusive DNA end-processing pathways. |
| doi_str_mv | 10.1073/pnas.1305166110 |
| format | Article |
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| subjects | Biological Sciences Deoxyribonucleic acid DNA DNA Breaks, Single-Stranded DNA, Fungal - metabolism DNA-Binding Proteins - metabolism Endodeoxyribonucleases - metabolism Exodeoxyribonucleases - metabolism Mutation PNAS Plus Proteins Replication Protein A - metabolism Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae Proteins - metabolism Yeast |
| title | Relationship of DNA degradation by Saccharomyces cerevisiae Exonuclease 1 and its stimulation by RPA and Mre11-Rad50-Xrs2 to DNA end resection |
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