pyrG is required for maintaining stable cellular uracil level and normal sporulation pattern under excess uracil stress in Aspergillus nidulans
Tight control of the intracellular uracil level is believed to be important to reduce the occurrence of uracil incorporation into DNA. The pyrG gene ofAspergillus niduIans encodes orotidine 5＇-phosphate decarboxylase, which catalyzes the conversion of orotidine monophosphate （OMP） to uridine monopho...
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| Veröffentlicht in: | Science China. Life sciences 2013-05, Vol.56 (5), p.467-475 |
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| creator | Sun, XianYun Zhu, JuFen Bao, Li Hu, ChengCheng Jin, Cheng Harris, Steven D. Liu, HongWei Li, ShaoJie |
| description | Tight control of the intracellular uracil level is believed to be important to reduce the occurrence of uracil incorporation into DNA. The pyrG gene ofAspergillus niduIans encodes orotidine 5＇-phosphate decarboxylase, which catalyzes the conversion of orotidine monophosphate （OMP） to uridine monophosphate （UMP）. In this study, we found that pyrG is critical for maintaining uracil at a low concentration in A. nidulans cells in the presence of exogenous uracil. Excess uracil and its derivatives had a stronger inhibitory effect on the growth of the pyrG89 mutant with defective OMP decarboxylase activity than on the growth of wild type, and induced sexual development in the pyrG89 mutant but not in wild type. Analysis of transcriptomic responses to excess uracil by digital gene expression profiling （DGE） revealed that genes related to sexual development were transcrip- tionally activated in the pyrG89 mutant but not in wild type. Quantitative analysis by HPLC showed that the cellular uracil level was 6.5 times higher in the pyrG89 mutant than in wild type in the presence of exogenous uracil. This study not only provides new information on uracil recycling and adaptation to excess uracil but also reveals the potential effects of OMP decarboxylase on fungal growth and development. |
| doi_str_mv | 10.1007/s11427-013-4480-6 |
| format | Article |
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The pyrG gene ofAspergillus niduIans encodes orotidine 5＇-phosphate decarboxylase, which catalyzes the conversion of orotidine monophosphate （OMP） to uridine monophosphate （UMP）. In this study, we found that pyrG is critical for maintaining uracil at a low concentration in A. nidulans cells in the presence of exogenous uracil. Excess uracil and its derivatives had a stronger inhibitory effect on the growth of the pyrG89 mutant with defective OMP decarboxylase activity than on the growth of wild type, and induced sexual development in the pyrG89 mutant but not in wild type. Analysis of transcriptomic responses to excess uracil by digital gene expression profiling （DGE） revealed that genes related to sexual development were transcrip- tionally activated in the pyrG89 mutant but not in wild type. Quantitative analysis by HPLC showed that the cellular uracil level was 6.5 times higher in the pyrG89 mutant than in wild type in the presence of exogenous uracil. This study not only provides new information on uracil recycling and adaptation to excess uracil but also reveals the potential effects of OMP decarboxylase on fungal growth and development.</description><identifier>ISSN: 1674-7305</identifier><identifier>EISSN: 1869-1889</identifier><identifier>DOI: 10.1007/s11427-013-4480-6</identifier><identifier>PMID: 23633078</identifier><language>eng</language><publisher>Beijing: Science China Press</publisher><subject>Aspergillus nidulans - genetics ; Aspergillus nidulans - metabolism ; Aspergillus nidulans - physiology ; Biomedical and Life Sciences ; Chromatography, High Pressure Liquid ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Fungal - drug effects ; Life Sciences ; Microscopy, Electron, Scanning ; Mutation ; Orotidine-5'-Phosphate Decarboxylase - genetics ; Orotidine-5'-Phosphate Decarboxylase - metabolism ; Research Paper ; Spores, Fungal - genetics ; Spores, Fungal - metabolism ; Spores, Fungal - physiology ; Uracil - metabolism ; Uracil - pharmacology ; Uridine Monophosphate - analogs & derivatives ; Uridine Monophosphate - metabolism ; 产孢 ; 压力 ; 发育相关基因 ; 尿嘧啶 ; 构巢曲霉 ; 水 ; 稳定 ; 细胞内</subject><ispartof>Science China. Life sciences, 2013-05, Vol.56 (5), p.467-475</ispartof><rights>The Author(s) 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-97866b1de7048e89de9b77b5f3eccddbe8c255456b48da499eeb5ff3988991293</citedby><cites>FETCH-LOGICAL-c408t-97866b1de7048e89de9b77b5f3eccddbe8c255456b48da499eeb5ff3988991293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/60112X/60112X.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11427-013-4480-6$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11427-013-4480-6$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23633078$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, XianYun</creatorcontrib><creatorcontrib>Zhu, JuFen</creatorcontrib><creatorcontrib>Bao, Li</creatorcontrib><creatorcontrib>Hu, ChengCheng</creatorcontrib><creatorcontrib>Jin, Cheng</creatorcontrib><creatorcontrib>Harris, Steven D.</creatorcontrib><creatorcontrib>Liu, HongWei</creatorcontrib><creatorcontrib>Li, ShaoJie</creatorcontrib><title>pyrG is required for maintaining stable cellular uracil level and normal sporulation pattern under excess uracil stress in Aspergillus nidulans</title><title>Science China. Life sciences</title><addtitle>Sci. China Life Sci</addtitle><addtitle>Sci China Life Sci</addtitle><description>Tight control of the intracellular uracil level is believed to be important to reduce the occurrence of uracil incorporation into DNA. The pyrG gene ofAspergillus niduIans encodes orotidine 5＇-phosphate decarboxylase, which catalyzes the conversion of orotidine monophosphate （OMP） to uridine monophosphate （UMP）. In this study, we found that pyrG is critical for maintaining uracil at a low concentration in A. nidulans cells in the presence of exogenous uracil. Excess uracil and its derivatives had a stronger inhibitory effect on the growth of the pyrG89 mutant with defective OMP decarboxylase activity than on the growth of wild type, and induced sexual development in the pyrG89 mutant but not in wild type. Analysis of transcriptomic responses to excess uracil by digital gene expression profiling （DGE） revealed that genes related to sexual development were transcrip- tionally activated in the pyrG89 mutant but not in wild type. Quantitative analysis by HPLC showed that the cellular uracil level was 6.5 times higher in the pyrG89 mutant than in wild type in the presence of exogenous uracil. This study not only provides new information on uracil recycling and adaptation to excess uracil but also reveals the potential effects of OMP decarboxylase on fungal growth and development.</description><subject>Aspergillus nidulans - genetics</subject><subject>Aspergillus nidulans - metabolism</subject><subject>Aspergillus nidulans - physiology</subject><subject>Biomedical and Life Sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Fungal - drug effects</subject><subject>Life Sciences</subject><subject>Microscopy, Electron, Scanning</subject><subject>Mutation</subject><subject>Orotidine-5'-Phosphate Decarboxylase - genetics</subject><subject>Orotidine-5'-Phosphate Decarboxylase - metabolism</subject><subject>Research Paper</subject><subject>Spores, Fungal - genetics</subject><subject>Spores, Fungal - metabolism</subject><subject>Spores, Fungal - physiology</subject><subject>Uracil - metabolism</subject><subject>Uracil - pharmacology</subject><subject>Uridine Monophosphate - analogs & derivatives</subject><subject>Uridine Monophosphate - metabolism</subject><subject>产孢</subject><subject>压力</subject><subject>发育相关基因</subject><subject>尿嘧啶</subject><subject>构巢曲霉</subject><subject>水</subject><subject>稳定</subject><subject>细胞内</subject><issn>1674-7305</issn><issn>1869-1889</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UU1r3DAQFaUlCWl-QC5FpWe3kiXr4xhCmxYCvSRnIVvjrYJW9o7s0vyK_OVo2U3oKYJBM8x7b4Z5hFxy9pUzpr8VzmWrG8ZFI6VhjXpHzrhRtuHG2Pc1V1o2WrDulFyU8sDqE4K1Wp-Q01aommtzRp7mR7yhsVCE3RoRAh0npFsf81Ij5g0ti-8T0AFSWpNHuqIfYqIJ_kKiPgeaJ9z6RMs8YQUsccp09ssCmOmaAyCFfwOU8kIsC-6rmOlVmQE3seoWmmOo5Fw-kg-jTwUujv85uf_x_e76Z3P7--bX9dVtM0hmlsZqo1TPA2gmDRgbwPZa990oYBhC6MEMbdfJTvXSBC-tBajNUdh6G8tbK87Jl4PujNNuhbK4h2nFXEc6LqQWSjPFKoofUANOpSCMbsa49fjoOHN7F9zBBVddcHsXnKqcT0fltd9CeGW83LwC2gOg1FbeAP43-g3Vz8dN_kx5s6u8V2HZdVpbpcQzicGg1A</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Sun, XianYun</creator><creator>Zhu, JuFen</creator><creator>Bao, Li</creator><creator>Hu, ChengCheng</creator><creator>Jin, Cheng</creator><creator>Harris, Steven D.</creator><creator>Liu, HongWei</creator><creator>Li, ShaoJie</creator><general>Science China Press</general><general>Springer Nature B.V</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>~WA</scope><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20130501</creationdate><title>pyrG is required for maintaining stable cellular uracil level and normal sporulation pattern under excess uracil stress in Aspergillus nidulans</title><author>Sun, XianYun ; 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Life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, XianYun</au><au>Zhu, JuFen</au><au>Bao, Li</au><au>Hu, ChengCheng</au><au>Jin, Cheng</au><au>Harris, Steven D.</au><au>Liu, HongWei</au><au>Li, ShaoJie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>pyrG is required for maintaining stable cellular uracil level and normal sporulation pattern under excess uracil stress in Aspergillus nidulans</atitle><jtitle>Science China. Life sciences</jtitle><stitle>Sci. China Life Sci</stitle><addtitle>Sci China Life Sci</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>56</volume><issue>5</issue><spage>467</spage><epage>475</epage><pages>467-475</pages><issn>1674-7305</issn><eissn>1869-1889</eissn><abstract>Tight control of the intracellular uracil level is believed to be important to reduce the occurrence of uracil incorporation into DNA. The pyrG gene ofAspergillus niduIans encodes orotidine 5＇-phosphate decarboxylase, which catalyzes the conversion of orotidine monophosphate （OMP） to uridine monophosphate （UMP）. In this study, we found that pyrG is critical for maintaining uracil at a low concentration in A. nidulans cells in the presence of exogenous uracil. Excess uracil and its derivatives had a stronger inhibitory effect on the growth of the pyrG89 mutant with defective OMP decarboxylase activity than on the growth of wild type, and induced sexual development in the pyrG89 mutant but not in wild type. Analysis of transcriptomic responses to excess uracil by digital gene expression profiling （DGE） revealed that genes related to sexual development were transcrip- tionally activated in the pyrG89 mutant but not in wild type. Quantitative analysis by HPLC showed that the cellular uracil level was 6.5 times higher in the pyrG89 mutant than in wild type in the presence of exogenous uracil. This study not only provides new information on uracil recycling and adaptation to excess uracil but also reveals the potential effects of OMP decarboxylase on fungal growth and development.</abstract><cop>Beijing</cop><pub>Science China Press</pub><pmid>23633078</pmid><doi>10.1007/s11427-013-4480-6</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1674-7305 |
| ispartof | Science China. Life sciences, 2013-05, Vol.56 (5), p.467-475 |
| issn | 1674-7305 1869-1889 |
| language | eng |
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| subjects | Aspergillus nidulans - genetics Aspergillus nidulans - metabolism Aspergillus nidulans - physiology Biomedical and Life Sciences Chromatography, High Pressure Liquid Fungal Proteins - genetics Fungal Proteins - metabolism Gene Expression Profiling Gene Expression Regulation, Fungal - drug effects Life Sciences Microscopy, Electron, Scanning Mutation Orotidine-5'-Phosphate Decarboxylase - genetics Orotidine-5'-Phosphate Decarboxylase - metabolism Research Paper Spores, Fungal - genetics Spores, Fungal - metabolism Spores, Fungal - physiology Uracil - metabolism Uracil - pharmacology Uridine Monophosphate - analogs & derivatives Uridine Monophosphate - metabolism 产孢 压力 发育相关基因 尿嘧啶 构巢曲霉 水 稳定 细胞内 |
| title | pyrG is required for maintaining stable cellular uracil level and normal sporulation pattern under excess uracil stress in Aspergillus nidulans |
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