In vivo and in vitro anti-angiogenesis effect of venom-derived peptides (ICD-85)
Agiogenesis is the development of new blood vessels from pre-existing vasculatures. Although essential in the physiological process, it becomes pathological in various diseases including cancer. Preventing the formation of new blood vessels causes reductions in tumor size and metastasis. This study...
Gespeichert in:
| Veröffentlicht in: | Archives of Iranian medicine 2013-02, Vol.16 (2), p.109 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 2 |
| container_start_page | 109 |
| container_title | Archives of Iranian medicine |
| container_volume | 16 |
| creator | Mombeinipour, Mandana Zare Mirakabadi, Abbas Mansuri, Kamran Lotfi, Mohsen |
| description | Agiogenesis is the development of new blood vessels from pre-existing vasculatures. Although essential in the physiological process, it becomes pathological in various diseases including cancer. Preventing the formation of new blood vessels causes reductions in tumor size and metastasis. This study has been undertaken to elucidate the anti-angiogenesis effects of ICD-85 (derived peptides from venom).
We evaluated the ICD-85 anti-angiogenesis activity by the in vivo CAM assay and in vitro tube formation assay of human umbilical vein endothelial cells (HUVECs). The anti-proliferative activity of ICD-85 was also determined through MTT assay on HUVECs.
Results of this study revealed the anti-proliferative activity of ICD-85 on the HUVEC cell line with an IC50 of 12 μg/mL. The in vivo CAM assay also clearly showed the prevention of new vascular formation when the chick embryos were exposed to 0.15 µg/disc of ICD-85. In vitro tube formation assay of HUVECs also showed the complete prevention of capillary tube formation on 18 µg/mL.
Based on the results obtained in this study, ICD-85 has anti-angiogenesis activity as shown by the prevention of capillary tube formation and the CAM assay. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_journals_1346914268</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2957813181</sourcerecordid><originalsourceid>FETCH-LOGICAL-p136t-27a03eddb284e336cd73947ef690220fa4a576d72ebdd07811962aaeab7024593</originalsourceid><addsrcrecordid>eNo1UM1KxDAYDKK46-orSMCLHgL5a9IcpbpaWNCDnku6-bJksU1t2oJvb4vraWZgmBnmDK2ZFhkRRurzmVNuCDdar9BVSkdKpciYuEQrLoSiSsg1ei9bPIUpYts6HBY-9IsYArHtIcQDtJBCwuA97AccPZ6gjQ1x0IcJHO6gG4KDhO_L4onk2cM1uvD2K8HNCTfoc_v8UbyS3dtLWTzuSMeEGgjXlgpwrua5hHnN3ullNHhlKOfUW2kzrZzmUDtHdc6YUdxasLWmXGZGbNDdX27Xx-8R0lAd49i3c2XFhFSGSa7y2XV7co11A67q-tDY_qf6P0D8ApiuVec</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1346914268</pqid></control><display><type>article</type><title>In vivo and in vitro anti-angiogenesis effect of venom-derived peptides (ICD-85)</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Mombeinipour, Mandana ; Zare Mirakabadi, Abbas ; Mansuri, Kamran ; Lotfi, Mohsen</creator><creatorcontrib>Mombeinipour, Mandana ; Zare Mirakabadi, Abbas ; Mansuri, Kamran ; Lotfi, Mohsen</creatorcontrib><description>Agiogenesis is the development of new blood vessels from pre-existing vasculatures. Although essential in the physiological process, it becomes pathological in various diseases including cancer. Preventing the formation of new blood vessels causes reductions in tumor size and metastasis. This study has been undertaken to elucidate the anti-angiogenesis effects of ICD-85 (derived peptides from venom).
We evaluated the ICD-85 anti-angiogenesis activity by the in vivo CAM assay and in vitro tube formation assay of human umbilical vein endothelial cells (HUVECs). The anti-proliferative activity of ICD-85 was also determined through MTT assay on HUVECs.
Results of this study revealed the anti-proliferative activity of ICD-85 on the HUVEC cell line with an IC50 of 12 μg/mL. The in vivo CAM assay also clearly showed the prevention of new vascular formation when the chick embryos were exposed to 0.15 µg/disc of ICD-85. In vitro tube formation assay of HUVECs also showed the complete prevention of capillary tube formation on 18 µg/mL.
Based on the results obtained in this study, ICD-85 has anti-angiogenesis activity as shown by the prevention of capillary tube formation and the CAM assay.</description><identifier>ISSN: 1029-2977</identifier><identifier>EISSN: 1735-3947</identifier><identifier>PMID: 23360634</identifier><language>eng</language><publisher>Iran: Academy of Medical Sciences of I.R. Iran</publisher><subject>Animals ; Cell Line ; Cell Proliferation - drug effects ; Chick Embryo ; Dose-Response Relationship, Drug ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Humans ; In Vitro Techniques ; Neovascularization, Pathologic - drug therapy ; Peptides - pharmacology ; Peptides - therapeutic use ; Umbilical Veins - cytology ; Umbilical Veins - drug effects ; Venoms - pharmacology ; Venoms - therapeutic use</subject><ispartof>Archives of Iranian medicine, 2013-02, Vol.16 (2), p.109</ispartof><rights>Copyright Academy of Medical Sciences of I.R. Iran Feb 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23360634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mombeinipour, Mandana</creatorcontrib><creatorcontrib>Zare Mirakabadi, Abbas</creatorcontrib><creatorcontrib>Mansuri, Kamran</creatorcontrib><creatorcontrib>Lotfi, Mohsen</creatorcontrib><title>In vivo and in vitro anti-angiogenesis effect of venom-derived peptides (ICD-85)</title><title>Archives of Iranian medicine</title><addtitle>Arch Iran Med</addtitle><description>Agiogenesis is the development of new blood vessels from pre-existing vasculatures. Although essential in the physiological process, it becomes pathological in various diseases including cancer. Preventing the formation of new blood vessels causes reductions in tumor size and metastasis. This study has been undertaken to elucidate the anti-angiogenesis effects of ICD-85 (derived peptides from venom).
We evaluated the ICD-85 anti-angiogenesis activity by the in vivo CAM assay and in vitro tube formation assay of human umbilical vein endothelial cells (HUVECs). The anti-proliferative activity of ICD-85 was also determined through MTT assay on HUVECs.
Results of this study revealed the anti-proliferative activity of ICD-85 on the HUVEC cell line with an IC50 of 12 μg/mL. The in vivo CAM assay also clearly showed the prevention of new vascular formation when the chick embryos were exposed to 0.15 µg/disc of ICD-85. In vitro tube formation assay of HUVECs also showed the complete prevention of capillary tube formation on 18 µg/mL.
Based on the results obtained in this study, ICD-85 has anti-angiogenesis activity as shown by the prevention of capillary tube formation and the CAM assay.</description><subject>Animals</subject><subject>Cell Line</subject><subject>Cell Proliferation - drug effects</subject><subject>Chick Embryo</subject><subject>Dose-Response Relationship, Drug</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Neovascularization, Pathologic - drug therapy</subject><subject>Peptides - pharmacology</subject><subject>Peptides - therapeutic use</subject><subject>Umbilical Veins - cytology</subject><subject>Umbilical Veins - drug effects</subject><subject>Venoms - pharmacology</subject><subject>Venoms - therapeutic use</subject><issn>1029-2977</issn><issn>1735-3947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNo1UM1KxDAYDKK46-orSMCLHgL5a9IcpbpaWNCDnku6-bJksU1t2oJvb4vraWZgmBnmDK2ZFhkRRurzmVNuCDdar9BVSkdKpciYuEQrLoSiSsg1ei9bPIUpYts6HBY-9IsYArHtIcQDtJBCwuA97AccPZ6gjQ1x0IcJHO6gG4KDhO_L4onk2cM1uvD2K8HNCTfoc_v8UbyS3dtLWTzuSMeEGgjXlgpwrua5hHnN3ullNHhlKOfUW2kzrZzmUDtHdc6YUdxasLWmXGZGbNDdX27Xx-8R0lAd49i3c2XFhFSGSa7y2XV7co11A67q-tDY_qf6P0D8ApiuVec</recordid><startdate>201302</startdate><enddate>201302</enddate><creator>Mombeinipour, Mandana</creator><creator>Zare Mirakabadi, Abbas</creator><creator>Mansuri, Kamran</creator><creator>Lotfi, Mohsen</creator><general>Academy of Medical Sciences of I.R. Iran</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>201302</creationdate><title>In vivo and in vitro anti-angiogenesis effect of venom-derived peptides (ICD-85)</title><author>Mombeinipour, Mandana ; Zare Mirakabadi, Abbas ; Mansuri, Kamran ; Lotfi, Mohsen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p136t-27a03eddb284e336cd73947ef690220fa4a576d72ebdd07811962aaeab7024593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>Cell Proliferation - drug effects</topic><topic>Chick Embryo</topic><topic>Dose-Response Relationship, Drug</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Neovascularization, Pathologic - drug therapy</topic><topic>Peptides - pharmacology</topic><topic>Peptides - therapeutic use</topic><topic>Umbilical Veins - cytology</topic><topic>Umbilical Veins - drug effects</topic><topic>Venoms - pharmacology</topic><topic>Venoms - therapeutic use</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mombeinipour, Mandana</creatorcontrib><creatorcontrib>Zare Mirakabadi, Abbas</creatorcontrib><creatorcontrib>Mansuri, Kamran</creatorcontrib><creatorcontrib>Lotfi, Mohsen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Archives of Iranian medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mombeinipour, Mandana</au><au>Zare Mirakabadi, Abbas</au><au>Mansuri, Kamran</au><au>Lotfi, Mohsen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo and in vitro anti-angiogenesis effect of venom-derived peptides (ICD-85)</atitle><jtitle>Archives of Iranian medicine</jtitle><addtitle>Arch Iran Med</addtitle><date>2013-02</date><risdate>2013</risdate><volume>16</volume><issue>2</issue><spage>109</spage><pages>109-</pages><issn>1029-2977</issn><eissn>1735-3947</eissn><abstract>Agiogenesis is the development of new blood vessels from pre-existing vasculatures. Although essential in the physiological process, it becomes pathological in various diseases including cancer. Preventing the formation of new blood vessels causes reductions in tumor size and metastasis. This study has been undertaken to elucidate the anti-angiogenesis effects of ICD-85 (derived peptides from venom).
We evaluated the ICD-85 anti-angiogenesis activity by the in vivo CAM assay and in vitro tube formation assay of human umbilical vein endothelial cells (HUVECs). The anti-proliferative activity of ICD-85 was also determined through MTT assay on HUVECs.
Results of this study revealed the anti-proliferative activity of ICD-85 on the HUVEC cell line with an IC50 of 12 μg/mL. The in vivo CAM assay also clearly showed the prevention of new vascular formation when the chick embryos were exposed to 0.15 µg/disc of ICD-85. In vitro tube formation assay of HUVECs also showed the complete prevention of capillary tube formation on 18 µg/mL.
Based on the results obtained in this study, ICD-85 has anti-angiogenesis activity as shown by the prevention of capillary tube formation and the CAM assay.</abstract><cop>Iran</cop><pub>Academy of Medical Sciences of I.R. Iran</pub><pmid>23360634</pmid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1029-2977 |
| ispartof | Archives of Iranian medicine, 2013-02, Vol.16 (2), p.109 |
| issn | 1029-2977 1735-3947 |
| language | eng |
| recordid | cdi_proquest_journals_1346914268 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Cell Line Cell Proliferation - drug effects Chick Embryo Dose-Response Relationship, Drug Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Humans In Vitro Techniques Neovascularization, Pathologic - drug therapy Peptides - pharmacology Peptides - therapeutic use Umbilical Veins - cytology Umbilical Veins - drug effects Venoms - pharmacology Venoms - therapeutic use |
| title | In vivo and in vitro anti-angiogenesis effect of venom-derived peptides (ICD-85) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T00%3A54%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vivo%20and%20in%20vitro%20anti-angiogenesis%20effect%20of%20venom-derived%20peptides%20(ICD-85)&rft.jtitle=Archives%20of%20Iranian%20medicine&rft.au=Mombeinipour,%20Mandana&rft.date=2013-02&rft.volume=16&rft.issue=2&rft.spage=109&rft.pages=109-&rft.issn=1029-2977&rft.eissn=1735-3947&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E2957813181%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1346914268&rft_id=info:pmid/23360634&rfr_iscdi=true |