Quantification of Field Resistance to Verticillium dahliae in Eight Russet-Skinned Potato Cultivars Using Real-Time PCR
Changes in potato production over the past 10 to 20 years, have resulted in increased emphasis being placed on breeding for resistance to Verticillium wilt, caused by Verticillium dahliae Kleb. While many russet-skinned cultivars recently have been released with reported resistance to Verticillium w...
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| Veröffentlicht in: | American journal of potato research 2013-04, Vol.90 (2), p.158-170 |
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| creator | Pasche, J. S. Thompson, A. L. Gudmestad, Neil C. |
| description | Changes in potato production over the past 10 to 20 years, have resulted in increased emphasis being placed on breeding for resistance to Verticillium wilt, caused by
Verticillium dahliae
Kleb. While many russet-skinned cultivars recently have been released with reported resistance to Verticillium wilt, information is lacking on the level of pathogen colonization, and therefore, the level of true genetic resistance is not known. Eight russet-skinned cultivars were grown in field trials with low and high levels of
V
.
dahliae
in the soil, and evaluated for wilt, stem colonization, yield, and tuber vascular discoloration. A recently developed QPCR assay was validated, with strong relationships to culture plating assays over three stem sampling dates. Additionally, stem colonization levels, as determined by QPCR, were related to wilt and tuber vascular discoloration. However, total yield did not exhibit a strong relationship to any other parameter evaluated in this study. Results from these studies indicate that varying levels of true resistance are present in the russet-skinned cultivars evaluated, and that the QPCR assay can be reliable in rapidly evaluating resistance to
V
.
dahliae
under field conditions. Based on pathogen quantification using stem colonization derived from traditional plating assays and QPCR, the resistance level of several cultivars is more clearly defined and discussed. |
| doi_str_mv | 10.1007/s12230-012-9280-1 |
| format | Article |
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Verticillium dahliae
Kleb. While many russet-skinned cultivars recently have been released with reported resistance to Verticillium wilt, information is lacking on the level of pathogen colonization, and therefore, the level of true genetic resistance is not known. Eight russet-skinned cultivars were grown in field trials with low and high levels of
V
.
dahliae
in the soil, and evaluated for wilt, stem colonization, yield, and tuber vascular discoloration. A recently developed QPCR assay was validated, with strong relationships to culture plating assays over three stem sampling dates. Additionally, stem colonization levels, as determined by QPCR, were related to wilt and tuber vascular discoloration. However, total yield did not exhibit a strong relationship to any other parameter evaluated in this study. Results from these studies indicate that varying levels of true resistance are present in the russet-skinned cultivars evaluated, and that the QPCR assay can be reliable in rapidly evaluating resistance to
V
.
dahliae
under field conditions. Based on pathogen quantification using stem colonization derived from traditional plating assays and QPCR, the resistance level of several cultivars is more clearly defined and discussed.</description><identifier>ISSN: 1099-209X</identifier><identifier>EISSN: 1874-9380</identifier><identifier>DOI: 10.1007/s12230-012-9280-1</identifier><identifier>CODEN: AJPRFQ</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Agriculture ; Agronomy. Soil science and plant productions ; Biological and medical sciences ; Biomedical and Life Sciences ; Colonization ; Cultivars ; Discoloration ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Genetics and breeding of economic plants ; Life Sciences ; Pathogens ; Phytopathology. Animal pests. Plant and forest protection ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Plant Pathology ; Plant Sciences ; Potatoes</subject><ispartof>American journal of potato research, 2013-04, Vol.90 (2), p.158-170</ispartof><rights>Potato Association of America 2012</rights><rights>2014 INIST-CNRS</rights><rights>Potato Association of America 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c346t-dab22ea41478897fe0c6accca27acdb13a8c0f5996b178ee6cc0c2ede566b5663</citedby><cites>FETCH-LOGICAL-c346t-dab22ea41478897fe0c6accca27acdb13a8c0f5996b178ee6cc0c2ede566b5663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12230-012-9280-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12230-012-9280-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27604989$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Pasche, J. S.</creatorcontrib><creatorcontrib>Thompson, A. L.</creatorcontrib><creatorcontrib>Gudmestad, Neil C.</creatorcontrib><title>Quantification of Field Resistance to Verticillium dahliae in Eight Russet-Skinned Potato Cultivars Using Real-Time PCR</title><title>American journal of potato research</title><addtitle>Am. J. Potato Res</addtitle><description>Changes in potato production over the past 10 to 20 years, have resulted in increased emphasis being placed on breeding for resistance to Verticillium wilt, caused by
Verticillium dahliae
Kleb. While many russet-skinned cultivars recently have been released with reported resistance to Verticillium wilt, information is lacking on the level of pathogen colonization, and therefore, the level of true genetic resistance is not known. Eight russet-skinned cultivars were grown in field trials with low and high levels of
V
.
dahliae
in the soil, and evaluated for wilt, stem colonization, yield, and tuber vascular discoloration. A recently developed QPCR assay was validated, with strong relationships to culture plating assays over three stem sampling dates. Additionally, stem colonization levels, as determined by QPCR, were related to wilt and tuber vascular discoloration. However, total yield did not exhibit a strong relationship to any other parameter evaluated in this study. Results from these studies indicate that varying levels of true resistance are present in the russet-skinned cultivars evaluated, and that the QPCR assay can be reliable in rapidly evaluating resistance to
V
.
dahliae
under field conditions. Based on pathogen quantification using stem colonization derived from traditional plating assays and QPCR, the resistance level of several cultivars is more clearly defined and discussed.</description><subject>Agriculture</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Colonization</subject><subject>Cultivars</subject><subject>Discoloration</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Genetics and breeding of economic plants</subject><subject>Life Sciences</subject><subject>Pathogens</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Potatoes</subject><issn>1099-209X</issn><issn>1874-9380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kE1rGzEQhkVIIYnTH5CboPSoVtKuV9KxmHwUDE3cpOS2jLWzzqRrbSJpG_LvK-NQculBjGDe95F4GDtT8ouS0nxNSutKCqm0cNpKoQ7YsbKmFq6y8rDcpXNCS3d_xE5SepRSK23nx-zlZoKQqScPmcbAx55fEA4dX2GilCF45HnkvzBm8jQMNG15Bw8DAXIK_Jw2D5mvppQwi5-_KQTs-PWYoXQW05DpD8TE7xKFTSHCIG5pi_x6sTplH3oYEn58mzN2d3F-u7gSyx-X3xfflsJXdZNFB2utEWpVG2ud6VH6Brz3oA34bq0qsF72c-eatTIWsfFeeo0dzptmXU41Y5_23Kc4Pk-Ycvs4TjGUJ1tV6Vobo50rKbVP-TimFLFvnyJtIb62SrY7v-3eb1v8tju_pTxjn9_IkDwMfSyuKP0ratPI2tkdW-9zqazCBuO7H_wX_hfJcYwB</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>Pasche, J. 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S. ; Thompson, A. L. ; Gudmestad, Neil C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c346t-dab22ea41478897fe0c6accca27acdb13a8c0f5996b178ee6cc0c2ede566b5663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Agriculture</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Colonization</topic><topic>Cultivars</topic><topic>Discoloration</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Genetics and breeding of economic plants</topic><topic>Life Sciences</topic><topic>Pathogens</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Potatoes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pasche, J. S.</creatorcontrib><creatorcontrib>Thompson, A. 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S.</au><au>Thompson, A. L.</au><au>Gudmestad, Neil C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of Field Resistance to Verticillium dahliae in Eight Russet-Skinned Potato Cultivars Using Real-Time PCR</atitle><jtitle>American journal of potato research</jtitle><stitle>Am. J. Potato Res</stitle><date>2013-04-01</date><risdate>2013</risdate><volume>90</volume><issue>2</issue><spage>158</spage><epage>170</epage><pages>158-170</pages><issn>1099-209X</issn><eissn>1874-9380</eissn><coden>AJPRFQ</coden><abstract>Changes in potato production over the past 10 to 20 years, have resulted in increased emphasis being placed on breeding for resistance to Verticillium wilt, caused by
Verticillium dahliae
Kleb. While many russet-skinned cultivars recently have been released with reported resistance to Verticillium wilt, information is lacking on the level of pathogen colonization, and therefore, the level of true genetic resistance is not known. Eight russet-skinned cultivars were grown in field trials with low and high levels of
V
.
dahliae
in the soil, and evaluated for wilt, stem colonization, yield, and tuber vascular discoloration. A recently developed QPCR assay was validated, with strong relationships to culture plating assays over three stem sampling dates. Additionally, stem colonization levels, as determined by QPCR, were related to wilt and tuber vascular discoloration. However, total yield did not exhibit a strong relationship to any other parameter evaluated in this study. Results from these studies indicate that varying levels of true resistance are present in the russet-skinned cultivars evaluated, and that the QPCR assay can be reliable in rapidly evaluating resistance to
V
.
dahliae
under field conditions. Based on pathogen quantification using stem colonization derived from traditional plating assays and QPCR, the resistance level of several cultivars is more clearly defined and discussed.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><doi>10.1007/s12230-012-9280-1</doi><tpages>13</tpages></addata></record> |
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| ispartof | American journal of potato research, 2013-04, Vol.90 (2), p.158-170 |
| issn | 1099-209X 1874-9380 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Agriculture Agronomy. Soil science and plant productions Biological and medical sciences Biomedical and Life Sciences Colonization Cultivars Discoloration Fundamental and applied biological sciences. Psychology Fungal plant pathogens Genetics and breeding of economic plants Life Sciences Pathogens Phytopathology. Animal pests. Plant and forest protection Plant Breeding/Biotechnology Plant Genetics and Genomics Plant Pathology Plant Sciences Potatoes |
| title | Quantification of Field Resistance to Verticillium dahliae in Eight Russet-Skinned Potato Cultivars Using Real-Time PCR |
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