Control by the embryo axis of the breakdown of storage proteins in cotyledons of germinating seeds of citrus limon
Azocaseolytic (endopeptidase) and carboxypeptidase activities in cotyledons of germinating seeds of Citrus limon (L) Burm f (cv Verna) showed a peak on the fourth day after the onset of imbibition and then decreased. A second peak of activity in both enzymes appeared 16 days after imbibition. The ac...
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| Veröffentlicht in: | Journal of the science of food and agriculture 1991, Vol.56 (4), p.435-443 |
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| creator | García-Agustín, Pilar Gastaldo, M José Benaches Primo-Millo, Eduardo |
| description | Azocaseolytic (endopeptidase) and carboxypeptidase activities in cotyledons of germinating seeds of Citrus limon (L) Burm f (cv Verna) showed a peak on the fourth day after the onset of imbibition and then decreased. A second peak of activity in both enzymes appeared 16 days after imbibition. The activities of leucine‐aminopeptidase and alanine‐aminopeptidase increased until 12 days after imbibition and then declined.
When cotyledons were detached from seedlings 8 days after imbibition and then incubated in water, protein breakdown was accelerated and α‐amino N accumulation increased. In detached and incubated cotyledons, the azocaseolytic activity decreased whereas the carboxypeptidase activity continued to increase more markedly than when the axis remained attached. The effect of the axis on azocaseolytic activity was replaceable by kinetin. On the other hand, kinetin, GA3 and IAA showed no significant effect on the carboxypeptidase and aminopeptidase activities in detached cotyledons. |
| doi_str_mv | 10.1002/jsfa.2740560404 |
| format | Article |
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When cotyledons were detached from seedlings 8 days after imbibition and then incubated in water, protein breakdown was accelerated and α‐amino N accumulation increased. In detached and incubated cotyledons, the azocaseolytic activity decreased whereas the carboxypeptidase activity continued to increase more markedly than when the axis remained attached. The effect of the axis on azocaseolytic activity was replaceable by kinetin. On the other hand, kinetin, GA3 and IAA showed no significant effect on the carboxypeptidase and aminopeptidase activities in detached cotyledons.</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.2740560404</identifier><identifier>CODEN: JSFAAE</identifier><language>eng</language><publisher>London: John Wiley & Sons, Ltd</publisher><subject>Aminopeptidase ; axis removal ; Biological and medical sciences ; carboxypeptidase ; Citrus ; endopeptidase ; Food industries ; Fruit and vegetable industries ; Fundamental and applied biological sciences. Psychology ; hormones ; seed germination</subject><ispartof>Journal of the science of food and agriculture, 1991, Vol.56 (4), p.435-443</ispartof><rights>Copyright © 1991 John Wiley & Sons, Ltd</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3884-4c8776b54cbe2f7c32bb436e1e987ec7d243468e40507009bb3fd5c4dfc34a183</citedby><cites>FETCH-LOGICAL-c3884-4c8776b54cbe2f7c32bb436e1e987ec7d243468e40507009bb3fd5c4dfc34a183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.2740560404$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.2740560404$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,4010,27850,27904,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5102803$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>García-Agustín, Pilar</creatorcontrib><creatorcontrib>Gastaldo, M José Benaches</creatorcontrib><creatorcontrib>Primo-Millo, Eduardo</creatorcontrib><title>Control by the embryo axis of the breakdown of storage proteins in cotyledons of germinating seeds of citrus limon</title><title>Journal of the science of food and agriculture</title><addtitle>J. Sci. Food Agric</addtitle><description>Azocaseolytic (endopeptidase) and carboxypeptidase activities in cotyledons of germinating seeds of Citrus limon (L) Burm f (cv Verna) showed a peak on the fourth day after the onset of imbibition and then decreased. A second peak of activity in both enzymes appeared 16 days after imbibition. The activities of leucine‐aminopeptidase and alanine‐aminopeptidase increased until 12 days after imbibition and then declined.
When cotyledons were detached from seedlings 8 days after imbibition and then incubated in water, protein breakdown was accelerated and α‐amino N accumulation increased. In detached and incubated cotyledons, the azocaseolytic activity decreased whereas the carboxypeptidase activity continued to increase more markedly than when the axis remained attached. The effect of the axis on azocaseolytic activity was replaceable by kinetin. On the other hand, kinetin, GA3 and IAA showed no significant effect on the carboxypeptidase and aminopeptidase activities in detached cotyledons.</description><subject>Aminopeptidase</subject><subject>axis removal</subject><subject>Biological and medical sciences</subject><subject>carboxypeptidase</subject><subject>Citrus</subject><subject>endopeptidase</subject><subject>Food industries</subject><subject>Fruit and vegetable industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hormones</subject><subject>seed germination</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>K30</sourceid><recordid>eNqFkEFPGzEQha2KSk1pz71aKteF8dq73qgnFAEtEECiVY-W7Z1NHTY22I5g_z2bBIF64jTS0_fezDxCvjE4ZADl0TJ1-rCUAqoaBIgPZMJgKgsABntkMhJlUTFRfiKfU1oCwHRa1xMSZ8HnGHpqBpr_IcWViUOg-sklGrqtZCLquzY8-o2Qcoh6gfQ-hozOJ-o8tSEPPbbBby0LjCvndXZ-QRNiuxWty3GdaO9WwX8hHzvdJ_z6MvfJn9OT37OfxeX12a_Z8WVhedOIQthGytpUwhosO2l5aYzgNTKcNhKtbEvBRd3g-DDI8R1jeNdWVrSd5UKzhu-T77vc8daHNaaslmEd_bhSMQ6cARONHKmjHWVjSClip-6jW-k4KAZqU6zaFKveih0dBy-5Olndd1F769KrrWJQNsBH7McOe3Q9Du-lqvPb0-P_lhQ7t0sZn17dOt6pWnJZqb9XZ4pdzOe385uZuuHPbZeaSw</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>García-Agustín, Pilar</creator><creator>Gastaldo, M José Benaches</creator><creator>Primo-Millo, Eduardo</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><general>Published for the Society of Chemical Industry by Elsevier Applied Science</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>HJHVS</scope><scope>IZSXY</scope><scope>K30</scope><scope>PAAUG</scope><scope>PAWHS</scope><scope>PAWZZ</scope><scope>PAXOH</scope><scope>PBHAV</scope><scope>PBQSW</scope><scope>PBYQZ</scope><scope>PCIWU</scope><scope>PCMID</scope><scope>PCZJX</scope><scope>PDGRG</scope><scope>PDWWI</scope><scope>PETMR</scope><scope>PFVGT</scope><scope>PGXDX</scope><scope>PIHIL</scope><scope>PISVA</scope><scope>PJCTQ</scope><scope>PJTMS</scope><scope>PLCHJ</scope><scope>PMHAD</scope><scope>PNQDJ</scope><scope>POUND</scope><scope>PPLAD</scope><scope>PQAPC</scope><scope>PQCAN</scope><scope>PQCMW</scope><scope>PQEME</scope><scope>PQHKH</scope><scope>PQMID</scope><scope>PQNCT</scope><scope>PQNET</scope><scope>PQSCT</scope><scope>PQSET</scope><scope>PSVJG</scope><scope>PVMQY</scope><scope>PZGFC</scope></search><sort><creationdate>1991</creationdate><title>Control by the embryo axis of the breakdown of storage proteins in cotyledons of germinating seeds of citrus limon</title><author>García-Agustín, Pilar ; Gastaldo, M José Benaches ; Primo-Millo, Eduardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3884-4c8776b54cbe2f7c32bb436e1e987ec7d243468e40507009bb3fd5c4dfc34a183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Aminopeptidase</topic><topic>axis removal</topic><topic>Biological and medical sciences</topic><topic>carboxypeptidase</topic><topic>Citrus</topic><topic>endopeptidase</topic><topic>Food industries</topic><topic>Fruit and vegetable industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hormones</topic><topic>seed germination</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>García-Agustín, Pilar</creatorcontrib><creatorcontrib>Gastaldo, M José Benaches</creatorcontrib><creatorcontrib>Primo-Millo, Eduardo</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Periodicals Index Online Segment 19</collection><collection>Periodicals Index Online Segment 30</collection><collection>Periodicals Index Online</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - West</collection><collection>Primary Sources Access (Plan D) - International</collection><collection>Primary Sources Access & Build (Plan A) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Midwest</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Northeast</collection><collection>Primary Sources Access (Plan D) - Southeast</collection><collection>Primary Sources Access (Plan D) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Southeast</collection><collection>Primary Sources Access (Plan D) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - UK / I</collection><collection>Primary Sources Access (Plan D) - Canada</collection><collection>Primary Sources Access (Plan D) - EMEALA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - International</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - International</collection><collection>Primary Sources Access (Plan D) - West</collection><collection>Periodicals Index Online Segments 1-50</collection><collection>Primary Sources Access (Plan D) - APAC</collection><collection>Primary Sources Access (Plan D) - Midwest</collection><collection>Primary Sources Access (Plan D) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Canada</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - EMEALA</collection><collection>Primary Sources Access & Build (Plan A) - APAC</collection><collection>Primary Sources Access & Build (Plan A) - Canada</collection><collection>Primary Sources Access & Build (Plan A) - West</collection><collection>Primary Sources Access & Build (Plan A) - EMEALA</collection><collection>Primary Sources Access (Plan D) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - Midwest</collection><collection>Primary Sources Access & Build (Plan A) - North Central</collection><collection>Primary Sources Access & Build (Plan A) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - Southeast</collection><collection>Primary Sources Access (Plan D) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - APAC</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - MEA</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García-Agustín, Pilar</au><au>Gastaldo, M José Benaches</au><au>Primo-Millo, Eduardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Control by the embryo axis of the breakdown of storage proteins in cotyledons of germinating seeds of citrus limon</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J. Sci. Food Agric</addtitle><date>1991</date><risdate>1991</risdate><volume>56</volume><issue>4</issue><spage>435</spage><epage>443</epage><pages>435-443</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><coden>JSFAAE</coden><abstract>Azocaseolytic (endopeptidase) and carboxypeptidase activities in cotyledons of germinating seeds of Citrus limon (L) Burm f (cv Verna) showed a peak on the fourth day after the onset of imbibition and then decreased. A second peak of activity in both enzymes appeared 16 days after imbibition. The activities of leucine‐aminopeptidase and alanine‐aminopeptidase increased until 12 days after imbibition and then declined.
When cotyledons were detached from seedlings 8 days after imbibition and then incubated in water, protein breakdown was accelerated and α‐amino N accumulation increased. In detached and incubated cotyledons, the azocaseolytic activity decreased whereas the carboxypeptidase activity continued to increase more markedly than when the axis remained attached. The effect of the axis on azocaseolytic activity was replaceable by kinetin. On the other hand, kinetin, GA3 and IAA showed no significant effect on the carboxypeptidase and aminopeptidase activities in detached cotyledons.</abstract><cop>London</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jsfa.2740560404</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of the science of food and agriculture, 1991, Vol.56 (4), p.435-443 |
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| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete; Periodicals Index Online |
| subjects | Aminopeptidase axis removal Biological and medical sciences carboxypeptidase Citrus endopeptidase Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology hormones seed germination |
| title | Control by the embryo axis of the breakdown of storage proteins in cotyledons of germinating seeds of citrus limon |
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