Rapid detection of polymorphism in yams (Dioscorea sp) through amplification by polymerase chain reaction and rDNA variation
Ribosomal DNA (rDNA) from rice (Oriza Sativa) was used to examine polymorphism in 12 yam (Dioscorea sp) cultivars. Restriction enzyme digests of total yam DNA was probed with the pRR217 probe containing the entire repeat unit of rDNA from rice. The polymerase chain reaction was also used to amplify...
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| Veröffentlicht in: | Journal of the science of food and agriculture 1995-03, Vol.67 (3), p.303-307 |
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| container_title | Journal of the science of food and agriculture |
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| creator | Muzac-Tucker, Ingrid Ahmad, Mohammed H |
| description | Ribosomal DNA (rDNA) from rice (Oriza Sativa) was used to examine polymorphism in 12 yam (Dioscorea sp) cultivars. Restriction enzyme digests of total yam DNA was probed with the pRR217 probe containing the entire repeat unit of rDNA from rice. The polymerase chain reaction was also used to amplify genomic DNA of six of the 12 cultivars studied using random primers. The amplification patterns of D rotundata‐cayenensis cv tau suggested that ‘tau’ is more closely related to D rotundata sp than it is to the D cayenensis sp. The results showed polymorphisms among the different yam cultivars. |
| doi_str_mv | 10.1002/jsfa.2740670305 |
| format | Article |
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Restriction enzyme digests of total yam DNA was probed with the pRR217 probe containing the entire repeat unit of rDNA from rice. The polymerase chain reaction was also used to amplify genomic DNA of six of the 12 cultivars studied using random primers. The amplification patterns of D rotundata‐cayenensis cv tau suggested that ‘tau’ is more closely related to D rotundata sp than it is to the D cayenensis sp. The results showed polymorphisms among the different yam cultivars.</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.2740670305</identifier><identifier>CODEN: JSFAAE</identifier><language>eng</language><publisher>London: John Wiley & Sons, Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; Dioscorea ; Fundamental and applied biological sciences. Psychology ; Generalities. Genetics. 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Sci. Food Agric</addtitle><description>Ribosomal DNA (rDNA) from rice (Oriza Sativa) was used to examine polymorphism in 12 yam (Dioscorea sp) cultivars. Restriction enzyme digests of total yam DNA was probed with the pRR217 probe containing the entire repeat unit of rDNA from rice. The polymerase chain reaction was also used to amplify genomic DNA of six of the 12 cultivars studied using random primers. The amplification patterns of D rotundata‐cayenensis cv tau suggested that ‘tau’ is more closely related to D rotundata sp than it is to the D cayenensis sp. The results showed polymorphisms among the different yam cultivars.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Dioscorea</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Genetics. Plant material</subject><subject>Genetic resources, diversity</subject><subject>Genetics and breeding of economic plants</subject><subject>Plant material</subject><subject>polymerase chain reaction</subject><subject>polymorphism</subject><subject>restriction fragment length polymorphism</subject><subject>yam</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>K30</sourceid><recordid>eNqFkEtv1DAURi1EJYbCmq0lWNBFWj9iOxar0ZQOoKqteEpsrDuOzXhI4mBngEj8eNKmKmLV1d2cc670IfSMkmNKCDvZZQ_HTJVEKsKJeIAWlGhVEELJQ7SYCFYIWrJH6HHOO0KI1lIu0J_30Ica125wdgixw9HjPjZjG1O_DbnFocMjtBm_PA0x25gc4Nwf4WGb4v7bFkPbN8EHCzfyZpxllyA7bLcw2ZMxl6GrcTq9WOKfkMIN_wQdeGiye3p7D9Gns9cfV2-K88v129XyvLC8kqLgjjGhRbURSmjNGJWO2woUccKXnNbCcV_KUkhNQXEuvdrImmolPfe-ksAP0fO526f4Y-_yYHZxn7rppaF8GktLJqqJOpkpm2LOyXnTp9BCGg0l5npicz2x-TfxZLy47UK20PgEnQ35TuMlLytCJuzVjP0KjRvvq5p3H86W_z0pZjvkwf2-syF9N1JxJcyXi7W5Uiu1_vp5bRj_C1OWnLk</recordid><startdate>199503</startdate><enddate>199503</enddate><creator>Muzac-Tucker, Ingrid</creator><creator>Ahmad, Mohammed H</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><general>Published for the Society of Chemical Industry by Elsevier Applied Science</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>HJHVS</scope><scope>IZSXY</scope><scope>K30</scope><scope>PAAUG</scope><scope>PAWHS</scope><scope>PAWZZ</scope><scope>PAXOH</scope><scope>PBHAV</scope><scope>PBQSW</scope><scope>PBYQZ</scope><scope>PCIWU</scope><scope>PCMID</scope><scope>PCZJX</scope><scope>PDGRG</scope><scope>PDWWI</scope><scope>PETMR</scope><scope>PFVGT</scope><scope>PGXDX</scope><scope>PIHIL</scope><scope>PISVA</scope><scope>PJCTQ</scope><scope>PJTMS</scope><scope>PLCHJ</scope><scope>PMHAD</scope><scope>PNQDJ</scope><scope>POUND</scope><scope>PPLAD</scope><scope>PQAPC</scope><scope>PQCAN</scope><scope>PQCMW</scope><scope>PQEME</scope><scope>PQHKH</scope><scope>PQMID</scope><scope>PQNCT</scope><scope>PQNET</scope><scope>PQSCT</scope><scope>PQSET</scope><scope>PSVJG</scope><scope>PVMQY</scope><scope>PZGFC</scope></search><sort><creationdate>199503</creationdate><title>Rapid detection of polymorphism in yams (Dioscorea sp) through amplification by polymerase chain reaction and rDNA variation</title><author>Muzac-Tucker, Ingrid ; Ahmad, Mohammed H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3865-3e225958b575992216e3c8a70e5f431d5e3f4645691a7336f7b6d1976f3ff86a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Dioscorea</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Genetics. Plant material</topic><topic>Genetic resources, diversity</topic><topic>Genetics and breeding of economic plants</topic><topic>Plant material</topic><topic>polymerase chain reaction</topic><topic>polymorphism</topic><topic>restriction fragment length polymorphism</topic><topic>yam</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muzac-Tucker, Ingrid</creatorcontrib><creatorcontrib>Ahmad, Mohammed H</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Periodicals Index Online Segment 19</collection><collection>Periodicals Index Online Segment 30</collection><collection>Periodicals Index Online</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - West</collection><collection>Primary Sources Access (Plan D) - International</collection><collection>Primary Sources Access & Build (Plan A) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Midwest</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Northeast</collection><collection>Primary Sources Access (Plan D) - Southeast</collection><collection>Primary Sources Access (Plan D) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Southeast</collection><collection>Primary Sources Access (Plan D) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - UK / I</collection><collection>Primary Sources Access (Plan D) - Canada</collection><collection>Primary Sources Access (Plan D) - EMEALA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - International</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - International</collection><collection>Primary Sources Access (Plan D) - West</collection><collection>Periodicals Index Online Segments 1-50</collection><collection>Primary Sources Access (Plan D) - APAC</collection><collection>Primary Sources Access (Plan D) - Midwest</collection><collection>Primary Sources Access (Plan D) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Canada</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - EMEALA</collection><collection>Primary Sources Access & Build (Plan A) - APAC</collection><collection>Primary Sources Access & Build (Plan A) - Canada</collection><collection>Primary Sources Access & Build (Plan A) - West</collection><collection>Primary Sources Access & Build (Plan A) - EMEALA</collection><collection>Primary Sources Access (Plan D) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - Midwest</collection><collection>Primary Sources Access & Build (Plan A) - North Central</collection><collection>Primary Sources Access & Build (Plan A) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - Southeast</collection><collection>Primary Sources Access (Plan D) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - APAC</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - MEA</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muzac-Tucker, Ingrid</au><au>Ahmad, Mohammed H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of polymorphism in yams (Dioscorea sp) through amplification by polymerase chain reaction and rDNA variation</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J. Sci. Food Agric</addtitle><date>1995-03</date><risdate>1995</risdate><volume>67</volume><issue>3</issue><spage>303</spage><epage>307</epage><pages>303-307</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><coden>JSFAAE</coden><abstract>Ribosomal DNA (rDNA) from rice (Oriza Sativa) was used to examine polymorphism in 12 yam (Dioscorea sp) cultivars. Restriction enzyme digests of total yam DNA was probed with the pRR217 probe containing the entire repeat unit of rDNA from rice. The polymerase chain reaction was also used to amplify genomic DNA of six of the 12 cultivars studied using random primers. The amplification patterns of D rotundata‐cayenensis cv tau suggested that ‘tau’ is more closely related to D rotundata sp than it is to the D cayenensis sp. The results showed polymorphisms among the different yam cultivars.</abstract><cop>London</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jsfa.2740670305</doi><tpages>5</tpages></addata></record> |
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| source | Periodicals Index Online; Access via Wiley Online Library |
| subjects | Agronomy. Soil science and plant productions Biological and medical sciences Dioscorea Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genetic resources, diversity Genetics and breeding of economic plants Plant material polymerase chain reaction polymorphism restriction fragment length polymorphism yam |
| title | Rapid detection of polymorphism in yams (Dioscorea sp) through amplification by polymerase chain reaction and rDNA variation |
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