Optimisation of techniques for quantification of Botrytis cinerea in grape berries and receptacles by quantitative polymerase chain reaction

Bunch rot symptoms can appear weeks after the infection of grape flowers by Botrytis cinerea. Quantitative polymerase chain reaction (qPCR) detects changes in the DNA mass of a target organism and is a potential tool for studying quiescent infections. The aim was to optimise a duplex qPCR to quantif...

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Veröffentlicht in:	Australian journal of grape and wine research 2013-02, Vol.19 (1), p.68-73
Hauptverfasser:	Saito, S, Dunne, K. J, Evans, K. J, Barry, K, Cadle-Davidson, L, Wilcox, W. F
Format:	Artikel
Sprache:	eng
Schlagworte:	Berries Botryotinia fuckeliana Botrytis cinerea conidia Deoxyribonucleic acid detection disease detection DNA DNA probes flowers Fruits fungal diseases of plants Fungicides grapes pathogen coefficient plant pathogenic fungi plant rots Polymerase chain reaction quantitative PCR Vitis vinifera
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