Magnetic Immunoassay for Detection of Staphylococcal Toxins in Complex Media
Method of highly sensitive registration of magnetic nanoparticles by their nonlinear magnetization is used in a novel sandwich-type immunoassay for detection of staphylococcal toxins in complex media of virtually any volume, with increasing sensitivity at higher sample volume. The signal is read out...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2013-01, Vol.85 (2), p.1154-1163 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Orlov, Alexey V Khodakova, Julia A Nikitin, Maxim P Shepelyakovskaya, Anna O Brovko, Fedor A Laman, Alexander G Grishin, Evgeny V Nikitin, Petr I |
| description | Method of highly sensitive registration of magnetic nanoparticles by their nonlinear magnetization is used in a novel sandwich-type immunoassay for detection of staphylococcal toxins in complex media of virtually any volume, with increasing sensitivity at higher sample volume. The signal is read out from the entire volume of a nontransparent 3D fiber structure employed as a solid phase, which provides large reaction surface, quick reagent mixing, as well as antigen immunofiltration directly in the course of the assay. The method has demonstrated near-linear dose–response curves within a wide range of ∼3 decades, while detection of staphylococcal enterotoxin A (SEA) and toxic shock syndrome toxin (TSST) in neat milk without sample preparation. The limits of detection (LOD) as low as 4 and 10 pg/mL for TSST and SEA, respectively, were obtained in 2-h format using 30-mL samples. The second, 25-min format, showed the LOD of 0.1 and 0.3 ng/mL for the same toxins in a 150 μL sample. The developed immunoassay can be applied in food safety control, in vitro diagnostics, and veterinary for a variety of research from express tests in the field to highly sensitive laboratory tests. |
| doi_str_mv | 10.1021/ac303075b |
| format | Article |
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Chem</addtitle><description>Method of highly sensitive registration of magnetic nanoparticles by their nonlinear magnetization is used in a novel sandwich-type immunoassay for detection of staphylococcal toxins in complex media of virtually any volume, with increasing sensitivity at higher sample volume. The signal is read out from the entire volume of a nontransparent 3D fiber structure employed as a solid phase, which provides large reaction surface, quick reagent mixing, as well as antigen immunofiltration directly in the course of the assay. The method has demonstrated near-linear dose–response curves within a wide range of ∼3 decades, while detection of staphylococcal enterotoxin A (SEA) and toxic shock syndrome toxin (TSST) in neat milk without sample preparation. The limits of detection (LOD) as low as 4 and 10 pg/mL for TSST and SEA, respectively, were obtained in 2-h format using 30-mL samples. The second, 25-min format, showed the LOD of 0.1 and 0.3 ng/mL for the same toxins in a 150 μL sample. 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Khodakova, Julia A ; Nikitin, Maxim P ; Shepelyakovskaya, Anna O ; Brovko, Fedor A ; Laman, Alexander G ; Grishin, Evgeny V ; Nikitin, Petr I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a343t-ce48bec3408aa3f8521f85f525f564e87d52b589aa35859d5b49f23b8ed04913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>Enterotoxins - analysis</topic><topic>Enterotoxins - genetics</topic><topic>Food safety</topic><topic>Immunoassay</topic><topic>Magnetite Nanoparticles - chemistry</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nanoparticles</topic><topic>Staphylococcus infections</topic><topic>Toxins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Orlov, Alexey V</creatorcontrib><creatorcontrib>Khodakova, Julia A</creatorcontrib><creatorcontrib>Nikitin, Maxim P</creatorcontrib><creatorcontrib>Shepelyakovskaya, Anna O</creatorcontrib><creatorcontrib>Brovko, Fedor A</creatorcontrib><creatorcontrib>Laman, Alexander G</creatorcontrib><creatorcontrib>Grishin, Evgeny V</creatorcontrib><creatorcontrib>Nikitin, Petr I</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Orlov, Alexey V</au><au>Khodakova, Julia A</au><au>Nikitin, Maxim P</au><au>Shepelyakovskaya, Anna O</au><au>Brovko, Fedor A</au><au>Laman, Alexander G</au><au>Grishin, Evgeny V</au><au>Nikitin, Petr I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Magnetic Immunoassay for Detection of Staphylococcal Toxins in Complex Media</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2013-01-15</date><risdate>2013</risdate><volume>85</volume><issue>2</issue><spage>1154</spage><epage>1163</epage><pages>1154-1163</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Method of highly sensitive registration of magnetic nanoparticles by their nonlinear magnetization is used in a novel sandwich-type immunoassay for detection of staphylococcal toxins in complex media of virtually any volume, with increasing sensitivity at higher sample volume. The signal is read out from the entire volume of a nontransparent 3D fiber structure employed as a solid phase, which provides large reaction surface, quick reagent mixing, as well as antigen immunofiltration directly in the course of the assay. The method has demonstrated near-linear dose–response curves within a wide range of ∼3 decades, while detection of staphylococcal enterotoxin A (SEA) and toxic shock syndrome toxin (TSST) in neat milk without sample preparation. 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| source | MEDLINE; American Chemical Society Journals |
| subjects | Animals Antigens Enterotoxins - analysis Enterotoxins - genetics Food safety Immunoassay Magnetite Nanoparticles - chemistry Mice Mice, Inbred BALB C Nanoparticles Staphylococcus infections Toxins |
| title | Magnetic Immunoassay for Detection of Staphylococcal Toxins in Complex Media |
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