Expression and regulation of ang-2 in murine ovaries during sexual maturation and development of corpus luteum
The aim of this study was to examine the expression and regulation of angiopoietin-2 (Ang-2) in murine ovaries during sexual maturation, gonadotropin treatment and luteal development by in situ hybridization and RT-PCR. By in situ hybridization Ang-2 mRNA was mainly localized in granulosa cells, the...
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| Veröffentlicht in: | Molecular biology (New York) 2012-11, Vol.46 (6), p.802-808 |
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| creator | Guo, B. Zhang, X. -M. Li, S. -J. Tian, X. -C. Wang, S. -T. Li, D. -D. Liu, D. -F. Yue, Z. -P. |
| description | The aim of this study was to examine the expression and regulation of angiopoietin-2 (Ang-2) in murine ovaries during sexual maturation, gonadotropin treatment and luteal development by in situ hybridization and RT-PCR. By in situ hybridization
Ang-2
mRNA was mainly localized in granulosa cells, thecal cells and corpus luteum, otherwise in oocytes. Moreover,
Ang-2
mRNA was highly expressed in corpus luteum and granulosa cells of atretic follicles. According to RT-PCR data,
Ang-2
mRNA was lowly expressed on day 10 after birth, then expression levels gradually increased and reached their highest values on day 25 after birth. In the superovulated model of immature mice,
Ang-2
expression was strongly induced by equine chorionic gonadotropin (eCG) 48 h post the eCG injection, and was high from 0.5 to 13 h after hCG treatment. in situ hybridization showed that
Ang-2
mRNA was highly expressed in corpus luteum from day 2 to 9 post the hCG injection, then the expression levels gradually declined on days 11 and 13 after hCG treatment. According to RT-PCR data, the levels of
Ang-2
mRNA expression showed a decline after the hCG injection, with a nadir on day 3, followed by an increase, reaching the highest level on day 9 post-hCG injection. Then again
Ang-2
expression gradually declined from day 11 to 15 after hCG injection. These results suggest that Ang-2 may be involved in follicular development, atresia, ovulation, and corpus luteum formation and regression. |
| doi_str_mv | 10.1134/S0026893312060076 |
| format | Article |
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Ang-2
mRNA was mainly localized in granulosa cells, thecal cells and corpus luteum, otherwise in oocytes. Moreover,
Ang-2
mRNA was highly expressed in corpus luteum and granulosa cells of atretic follicles. According to RT-PCR data,
Ang-2
mRNA was lowly expressed on day 10 after birth, then expression levels gradually increased and reached their highest values on day 25 after birth. In the superovulated model of immature mice,
Ang-2
expression was strongly induced by equine chorionic gonadotropin (eCG) 48 h post the eCG injection, and was high from 0.5 to 13 h after hCG treatment. in situ hybridization showed that
Ang-2
mRNA was highly expressed in corpus luteum from day 2 to 9 post the hCG injection, then the expression levels gradually declined on days 11 and 13 after hCG treatment. According to RT-PCR data, the levels of
Ang-2
mRNA expression showed a decline after the hCG injection, with a nadir on day 3, followed by an increase, reaching the highest level on day 9 post-hCG injection. Then again
Ang-2
expression gradually declined from day 11 to 15 after hCG injection. These results suggest that Ang-2 may be involved in follicular development, atresia, ovulation, and corpus luteum formation and regression.</description><identifier>ISSN: 0026-8933</identifier><identifier>EISSN: 1608-3245</identifier><identifier>DOI: 10.1134/S0026893312060076</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Flowers & plants ; Gene expression ; Human Genetics ; Hybridization ; Life Sciences ; Molecular Biology of The Cell</subject><ispartof>Molecular biology (New York), 2012-11, Vol.46 (6), p.802-808</ispartof><rights>Pleiades Publishing, Ltd. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-61744ad84e958bf423916bd47e8d0b1d7350cafe58fbd90f0321484998e8f0f03</citedby><cites>FETCH-LOGICAL-c316t-61744ad84e958bf423916bd47e8d0b1d7350cafe58fbd90f0321484998e8f0f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0026893312060076$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0026893312060076$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids></links><search><creatorcontrib>Guo, B.</creatorcontrib><creatorcontrib>Zhang, X. -M.</creatorcontrib><creatorcontrib>Li, S. -J.</creatorcontrib><creatorcontrib>Tian, X. -C.</creatorcontrib><creatorcontrib>Wang, S. -T.</creatorcontrib><creatorcontrib>Li, D. -D.</creatorcontrib><creatorcontrib>Liu, D. -F.</creatorcontrib><creatorcontrib>Yue, Z. -P.</creatorcontrib><title>Expression and regulation of ang-2 in murine ovaries during sexual maturation and development of corpus luteum</title><title>Molecular biology (New York)</title><addtitle>Mol Biol</addtitle><description>The aim of this study was to examine the expression and regulation of angiopoietin-2 (Ang-2) in murine ovaries during sexual maturation, gonadotropin treatment and luteal development by in situ hybridization and RT-PCR. By in situ hybridization
Ang-2
mRNA was mainly localized in granulosa cells, thecal cells and corpus luteum, otherwise in oocytes. Moreover,
Ang-2
mRNA was highly expressed in corpus luteum and granulosa cells of atretic follicles. According to RT-PCR data,
Ang-2
mRNA was lowly expressed on day 10 after birth, then expression levels gradually increased and reached their highest values on day 25 after birth. In the superovulated model of immature mice,
Ang-2
expression was strongly induced by equine chorionic gonadotropin (eCG) 48 h post the eCG injection, and was high from 0.5 to 13 h after hCG treatment. in situ hybridization showed that
Ang-2
mRNA was highly expressed in corpus luteum from day 2 to 9 post the hCG injection, then the expression levels gradually declined on days 11 and 13 after hCG treatment. According to RT-PCR data, the levels of
Ang-2
mRNA expression showed a decline after the hCG injection, with a nadir on day 3, followed by an increase, reaching the highest level on day 9 post-hCG injection. Then again
Ang-2
expression gradually declined from day 11 to 15 after hCG injection. These results suggest that Ang-2 may be involved in follicular development, atresia, ovulation, and corpus luteum formation and regression.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Flowers & plants</subject><subject>Gene expression</subject><subject>Human Genetics</subject><subject>Hybridization</subject><subject>Life Sciences</subject><subject>Molecular Biology of The Cell</subject><issn>0026-8933</issn><issn>1608-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UMtKxDAUDaLgOPoB7gKuq3k1TZcyjA8QXKjrkjY3Q4e2qUkzjH9vQ10IIly4nHseFw5C15TcUsrF3RshTKqSc8qIJKSQJ2hFJVEZZyI_RatEZ4k_Rxch7Amh87AVGrbH0UMIrRuwHgz2sIudnhJ0dr7sMobbAffRtwNgd9C-hYBNgjsc4Bh1h3s9Rb94UoSBA3Ru7GGYUkbj_BgD7uIEsb9EZ1Z3Aa5-9hp9PGzfN0_Zy-vj8-b-JWs4lVMmaSGENkpAmavaCsZLKmsjClCG1NQUPCeNtpArW5uSWMIZFUqUpQJlE1yjmyV39O4zQpiqvYt-mF9WlEkpBC2pmlV0UTXeheDBVqNve-2_KkqqVGv1p9bZwxZPGFMH4H8l_2v6BjZRek4</recordid><startdate>20121101</startdate><enddate>20121101</enddate><creator>Guo, B.</creator><creator>Zhang, X. -M.</creator><creator>Li, S. -J.</creator><creator>Tian, X. -C.</creator><creator>Wang, S. -T.</creator><creator>Li, D. -D.</creator><creator>Liu, D. -F.</creator><creator>Yue, Z. -P.</creator><general>SP MAIK Nauka/Interperiodica</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20121101</creationdate><title>Expression and regulation of ang-2 in murine ovaries during sexual maturation and development of corpus luteum</title><author>Guo, B. ; Zhang, X. -M. ; Li, S. -J. ; Tian, X. -C. ; Wang, S. -T. ; Li, D. -D. ; Liu, D. -F. ; Yue, Z. -P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-61744ad84e958bf423916bd47e8d0b1d7350cafe58fbd90f0321484998e8f0f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Flowers & plants</topic><topic>Gene expression</topic><topic>Human Genetics</topic><topic>Hybridization</topic><topic>Life Sciences</topic><topic>Molecular Biology of The Cell</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, B.</creatorcontrib><creatorcontrib>Zhang, X. -M.</creatorcontrib><creatorcontrib>Li, S. -J.</creatorcontrib><creatorcontrib>Tian, X. -C.</creatorcontrib><creatorcontrib>Wang, S. -T.</creatorcontrib><creatorcontrib>Li, D. -D.</creatorcontrib><creatorcontrib>Liu, D. -F.</creatorcontrib><creatorcontrib>Yue, Z. -P.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Molecular biology (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, B.</au><au>Zhang, X. -M.</au><au>Li, S. -J.</au><au>Tian, X. -C.</au><au>Wang, S. -T.</au><au>Li, D. -D.</au><au>Liu, D. -F.</au><au>Yue, Z. -P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and regulation of ang-2 in murine ovaries during sexual maturation and development of corpus luteum</atitle><jtitle>Molecular biology (New York)</jtitle><stitle>Mol Biol</stitle><date>2012-11-01</date><risdate>2012</risdate><volume>46</volume><issue>6</issue><spage>802</spage><epage>808</epage><pages>802-808</pages><issn>0026-8933</issn><eissn>1608-3245</eissn><abstract>The aim of this study was to examine the expression and regulation of angiopoietin-2 (Ang-2) in murine ovaries during sexual maturation, gonadotropin treatment and luteal development by in situ hybridization and RT-PCR. By in situ hybridization
Ang-2
mRNA was mainly localized in granulosa cells, thecal cells and corpus luteum, otherwise in oocytes. Moreover,
Ang-2
mRNA was highly expressed in corpus luteum and granulosa cells of atretic follicles. According to RT-PCR data,
Ang-2
mRNA was lowly expressed on day 10 after birth, then expression levels gradually increased and reached their highest values on day 25 after birth. In the superovulated model of immature mice,
Ang-2
expression was strongly induced by equine chorionic gonadotropin (eCG) 48 h post the eCG injection, and was high from 0.5 to 13 h after hCG treatment. in situ hybridization showed that
Ang-2
mRNA was highly expressed in corpus luteum from day 2 to 9 post the hCG injection, then the expression levels gradually declined on days 11 and 13 after hCG treatment. According to RT-PCR data, the levels of
Ang-2
mRNA expression showed a decline after the hCG injection, with a nadir on day 3, followed by an increase, reaching the highest level on day 9 post-hCG injection. Then again
Ang-2
expression gradually declined from day 11 to 15 after hCG injection. These results suggest that Ang-2 may be involved in follicular development, atresia, ovulation, and corpus luteum formation and regression.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S0026893312060076</doi><tpages>7</tpages></addata></record> |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Biochemistry Biomedical and Life Sciences Flowers & plants Gene expression Human Genetics Hybridization Life Sciences Molecular Biology of The Cell |
| title | Expression and regulation of ang-2 in murine ovaries during sexual maturation and development of corpus luteum |
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