Protein-Protected Au Clusters as a New Class of Nanoscale Biosensor for Label-Free Fluorescence Detection of Proteases

Protein-Protected Au Clusters as a New Class of Nanoscale Biosensor for Label-Free Fluorescence Detection of Proteases

Destruction for detection: Protein‐protected Au clusters are exploited as nanoscale sensors for label‐free fluorescence detection of proteases with high sensitivity. The detection mechanism is based on degradation of the protein shell by a protease, allowing the O2 from ambient air to penetrate thro...

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Veröffentlicht in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2012-12, Vol.8 (24), p.3769-3773
Hauptverfasser: Wang, Yucai, Wang, Yi, Zhou, Fengbo, Kim, Paul, Xia, Younan
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Au clusters
Biosensing Techniques - methods
biosensors
Cattle
Endopeptidase K - analysis
Fluorescence
fluorescence quenching
Fluorescent Dyes
Gold
label-free detection
Metal Nanoparticles
Nanotechnology
Peptide Hydrolases - analysis
proteases
Proteins
Serum Albumin, Bovine
Spectrometry, Fluorescence
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Zusammenfassung:Destruction for detection: Protein‐protected Au clusters are exploited as nanoscale sensors for label‐free fluorescence detection of proteases with high sensitivity. The detection mechanism is based on degradation of the protein shell by a protease, allowing the O2 from ambient air to penetrate through and quench the fluorescence from the Au cluster.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201201983