Characterization of an apo-carotenoid 13,14-dioxygenase from Novosphingobium aromaticivorans that converts [beta]-apo-8'-carotenal to [beta]-apo-13-carotenone

A putative carotenoid oxygenase from Novosphingobium aromaticivorans was purified with a specific activity of 0.8 U/mg by His-Trap affinity chromatography. The native enzyme was estimated to be a 52 kDa monomer. Enzyme activity for β-apo-8'-carotenal was maximal at pH 8.0 and 45 °C, with a half...

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Veröffentlicht in:	Biotechnology letters 2012-10, Vol.34 (10), p.1851
Hauptverfasser:	Kim, Yeong-su, Seo, Eun-sun, Oh, Deok-kun
Format:	Artikel
Sprache:	eng
Schlagworte:	Atoms & subatomic particles Biotechnology Carotenoids Enzymatic activity Enzymes Microorganisms
Online-Zugang:	Volltext
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