Distribution and ultrastructure of interstitial cells of Cajal in the gastric antrum of wild-type and Ws/Ws rats
Interstitial cells of Cajal (ICC) in the stomach of wild-type and Ws/Ws mutant rats that are deficient in c-kit were studied by immunohistochemistry and electron microscopy to elucidate their regional specialization in the gastric antrum. Immunohistochemistry for Kit protein demonstrated that in wil...
Gespeichert in:
| Veröffentlicht in: | Anatomy and Embryology 2003-05, Vol.206 (6), p.453-460 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 460 |
|---|---|
| container_issue | 6 |
| container_start_page | 453 |
| container_title | Anatomy and Embryology |
| container_volume | 206 |
| creator | Mitsui, Retsu Komuro, Terumasa |
| description | Interstitial cells of Cajal (ICC) in the stomach of wild-type and Ws/Ws mutant rats that are deficient in c-kit were studied by immunohistochemistry and electron microscopy to elucidate their regional specialization in the gastric antrum. Immunohistochemistry for Kit protein demonstrated that in wild-type rats ICC were located at the submucosal border of the circular muscle layer (ICC-SM) in a limited extension of the antrum from the pyloric sphincter towards the corpus, as well as within both the circular (ICC-CM) and longitudinal (ICC-LM) muscle layers and in the myenteric plexus region (ICC-AP). In c-kit mutant Ws/Ws rats while ICC-CM and ICC-LM were not observed, but unexpectedly, a few ICC-SM and ICC-AP were found. By electron microscopy, ICC-SM and ICC-AP were characterized by abundant mitochondria, many caveolae, a distinct basal lamina and formed gap junctions with other ICC or with smooth muscle cells and make close contacts with nerves. Thus, ICC-SM and ICC-AP of the rat antrum were classified as Type 3 ICC, the type most similar to smooth muscle cells. The functional significance of ICC-SM and their survival in the c-kit mutant animals is discussed in reference to the role of the c-kit/stem cell factor system for their cellular maturation. |
| doi_str_mv | 10.1007/s00429-003-0323-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1112073191</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2788904631</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-ce16e4e865349a8920a037b4fce4e14f7d8433addb22f421f02d856294a432613</originalsourceid><addsrcrecordid>eNpdkUtLxDAUhYMojo7-ADcSENzFuXm0TZcyPmHAjTLLkLapZujLJEXm35s6A4qrJCfnfFzuQeiCwg0FyBYeQLCcAHACnHEiD9AJcAGEQUoP_9xn6NT7DQBlkiXHaEZZBiDz_AQNd9YHZ4sx2L7Duqvw2ASnozaWYXQG9zW2XTDOBxusbnBpmsZP6lJv4tN2OHwY_D4lbBkBMdhO31-2qUjYDuYHuvaLtcdOB3-GjmrdeHO-P-fo7eH-dflEVi-Pz8vbFSl5TgMpDU2NMDJNuMi1zBlo4Fkh6jKqVNRZJQXnuqoKxmrBaA2skknKcqEFZynlc3S94w6u_xyND6q1fhped6Yfvco4ZxnjSTRe_TNu-tF1cTZFKWWQcZpPOLpzla733plaDc622m0VBTWVoXZlqFiGmspQMmYu9-SxaE31m9hvn38D_sKEiA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1112073191</pqid></control><display><type>article</type><title>Distribution and ultrastructure of interstitial cells of Cajal in the gastric antrum of wild-type and Ws/Ws rats</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Mitsui, Retsu ; Komuro, Terumasa</creator><creatorcontrib>Mitsui, Retsu ; Komuro, Terumasa</creatorcontrib><description>Interstitial cells of Cajal (ICC) in the stomach of wild-type and Ws/Ws mutant rats that are deficient in c-kit were studied by immunohistochemistry and electron microscopy to elucidate their regional specialization in the gastric antrum. Immunohistochemistry for Kit protein demonstrated that in wild-type rats ICC were located at the submucosal border of the circular muscle layer (ICC-SM) in a limited extension of the antrum from the pyloric sphincter towards the corpus, as well as within both the circular (ICC-CM) and longitudinal (ICC-LM) muscle layers and in the myenteric plexus region (ICC-AP). In c-kit mutant Ws/Ws rats while ICC-CM and ICC-LM were not observed, but unexpectedly, a few ICC-SM and ICC-AP were found. By electron microscopy, ICC-SM and ICC-AP were characterized by abundant mitochondria, many caveolae, a distinct basal lamina and formed gap junctions with other ICC or with smooth muscle cells and make close contacts with nerves. Thus, ICC-SM and ICC-AP of the rat antrum were classified as Type 3 ICC, the type most similar to smooth muscle cells. The functional significance of ICC-SM and their survival in the c-kit mutant animals is discussed in reference to the role of the c-kit/stem cell factor system for their cellular maturation.</description><identifier>ISSN: 0340-2061</identifier><identifier>ISSN: 1863-2653</identifier><identifier>EISSN: 0340-2061</identifier><identifier>DOI: 10.1007/s00429-003-0323-8</identifier><identifier>PMID: 12700899</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Animals ; Cell Differentiation ; Cell Survival ; Female ; Gastric Mucosa - immunology ; Gastric Mucosa - physiology ; Immunohistochemistry ; Male ; Microscopy, Electron ; Muscle, Smooth - physiology ; Muscle, Smooth - ultrastructure ; Muscular system ; Pyloric Antrum - cytology ; Pyloric Antrum - ultrastructure ; Rats ; Rodents ; Smooth muscle ; Stomach - cytology ; Stomach - physiology ; Stomach - ultrastructure</subject><ispartof>Anatomy and Embryology, 2003-05, Vol.206 (6), p.453-460</ispartof><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-ce16e4e865349a8920a037b4fce4e14f7d8433addb22f421f02d856294a432613</citedby><cites>FETCH-LOGICAL-c391t-ce16e4e865349a8920a037b4fce4e14f7d8433addb22f421f02d856294a432613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12700899$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitsui, Retsu</creatorcontrib><creatorcontrib>Komuro, Terumasa</creatorcontrib><title>Distribution and ultrastructure of interstitial cells of Cajal in the gastric antrum of wild-type and Ws/Ws rats</title><title>Anatomy and Embryology</title><addtitle>Anat Embryol (Berl)</addtitle><description>Interstitial cells of Cajal (ICC) in the stomach of wild-type and Ws/Ws mutant rats that are deficient in c-kit were studied by immunohistochemistry and electron microscopy to elucidate their regional specialization in the gastric antrum. Immunohistochemistry for Kit protein demonstrated that in wild-type rats ICC were located at the submucosal border of the circular muscle layer (ICC-SM) in a limited extension of the antrum from the pyloric sphincter towards the corpus, as well as within both the circular (ICC-CM) and longitudinal (ICC-LM) muscle layers and in the myenteric plexus region (ICC-AP). In c-kit mutant Ws/Ws rats while ICC-CM and ICC-LM were not observed, but unexpectedly, a few ICC-SM and ICC-AP were found. By electron microscopy, ICC-SM and ICC-AP were characterized by abundant mitochondria, many caveolae, a distinct basal lamina and formed gap junctions with other ICC or with smooth muscle cells and make close contacts with nerves. Thus, ICC-SM and ICC-AP of the rat antrum were classified as Type 3 ICC, the type most similar to smooth muscle cells. The functional significance of ICC-SM and their survival in the c-kit mutant animals is discussed in reference to the role of the c-kit/stem cell factor system for their cellular maturation.</description><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Cell Survival</subject><subject>Female</subject><subject>Gastric Mucosa - immunology</subject><subject>Gastric Mucosa - physiology</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Muscle, Smooth - physiology</subject><subject>Muscle, Smooth - ultrastructure</subject><subject>Muscular system</subject><subject>Pyloric Antrum - cytology</subject><subject>Pyloric Antrum - ultrastructure</subject><subject>Rats</subject><subject>Rodents</subject><subject>Smooth muscle</subject><subject>Stomach - cytology</subject><subject>Stomach - physiology</subject><subject>Stomach - ultrastructure</subject><issn>0340-2061</issn><issn>1863-2653</issn><issn>0340-2061</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkUtLxDAUhYMojo7-ADcSENzFuXm0TZcyPmHAjTLLkLapZujLJEXm35s6A4qrJCfnfFzuQeiCwg0FyBYeQLCcAHACnHEiD9AJcAGEQUoP_9xn6NT7DQBlkiXHaEZZBiDz_AQNd9YHZ4sx2L7Duqvw2ASnozaWYXQG9zW2XTDOBxusbnBpmsZP6lJv4tN2OHwY_D4lbBkBMdhO31-2qUjYDuYHuvaLtcdOB3-GjmrdeHO-P-fo7eH-dflEVi-Pz8vbFSl5TgMpDU2NMDJNuMi1zBlo4Fkh6jKqVNRZJQXnuqoKxmrBaA2skknKcqEFZynlc3S94w6u_xyND6q1fhped6Yfvco4ZxnjSTRe_TNu-tF1cTZFKWWQcZpPOLpzla733plaDc622m0VBTWVoXZlqFiGmspQMmYu9-SxaE31m9hvn38D_sKEiA</recordid><startdate>20030501</startdate><enddate>20030501</enddate><creator>Mitsui, Retsu</creator><creator>Komuro, Terumasa</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20030501</creationdate><title>Distribution and ultrastructure of interstitial cells of Cajal in the gastric antrum of wild-type and Ws/Ws rats</title><author>Mitsui, Retsu ; Komuro, Terumasa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-ce16e4e865349a8920a037b4fce4e14f7d8433addb22f421f02d856294a432613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Cell Differentiation</topic><topic>Cell Survival</topic><topic>Female</topic><topic>Gastric Mucosa - immunology</topic><topic>Gastric Mucosa - physiology</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>Muscle, Smooth - physiology</topic><topic>Muscle, Smooth - ultrastructure</topic><topic>Muscular system</topic><topic>Pyloric Antrum - cytology</topic><topic>Pyloric Antrum - ultrastructure</topic><topic>Rats</topic><topic>Rodents</topic><topic>Smooth muscle</topic><topic>Stomach - cytology</topic><topic>Stomach - physiology</topic><topic>Stomach - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitsui, Retsu</creatorcontrib><creatorcontrib>Komuro, Terumasa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Anatomy and Embryology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitsui, Retsu</au><au>Komuro, Terumasa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution and ultrastructure of interstitial cells of Cajal in the gastric antrum of wild-type and Ws/Ws rats</atitle><jtitle>Anatomy and Embryology</jtitle><addtitle>Anat Embryol (Berl)</addtitle><date>2003-05-01</date><risdate>2003</risdate><volume>206</volume><issue>6</issue><spage>453</spage><epage>460</epage><pages>453-460</pages><issn>0340-2061</issn><issn>1863-2653</issn><eissn>0340-2061</eissn><abstract>Interstitial cells of Cajal (ICC) in the stomach of wild-type and Ws/Ws mutant rats that are deficient in c-kit were studied by immunohistochemistry and electron microscopy to elucidate their regional specialization in the gastric antrum. Immunohistochemistry for Kit protein demonstrated that in wild-type rats ICC were located at the submucosal border of the circular muscle layer (ICC-SM) in a limited extension of the antrum from the pyloric sphincter towards the corpus, as well as within both the circular (ICC-CM) and longitudinal (ICC-LM) muscle layers and in the myenteric plexus region (ICC-AP). In c-kit mutant Ws/Ws rats while ICC-CM and ICC-LM were not observed, but unexpectedly, a few ICC-SM and ICC-AP were found. By electron microscopy, ICC-SM and ICC-AP were characterized by abundant mitochondria, many caveolae, a distinct basal lamina and formed gap junctions with other ICC or with smooth muscle cells and make close contacts with nerves. Thus, ICC-SM and ICC-AP of the rat antrum were classified as Type 3 ICC, the type most similar to smooth muscle cells. The functional significance of ICC-SM and their survival in the c-kit mutant animals is discussed in reference to the role of the c-kit/stem cell factor system for their cellular maturation.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>12700899</pmid><doi>10.1007/s00429-003-0323-8</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0340-2061 |
| ispartof | Anatomy and Embryology, 2003-05, Vol.206 (6), p.453-460 |
| issn | 0340-2061 1863-2653 0340-2061 |
| language | eng |
| recordid | cdi_proquest_journals_1112073191 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Cell Differentiation Cell Survival Female Gastric Mucosa - immunology Gastric Mucosa - physiology Immunohistochemistry Male Microscopy, Electron Muscle, Smooth - physiology Muscle, Smooth - ultrastructure Muscular system Pyloric Antrum - cytology Pyloric Antrum - ultrastructure Rats Rodents Smooth muscle Stomach - cytology Stomach - physiology Stomach - ultrastructure |
| title | Distribution and ultrastructure of interstitial cells of Cajal in the gastric antrum of wild-type and Ws/Ws rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T18%3A20%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Distribution%20and%20ultrastructure%20of%20interstitial%20cells%20of%20Cajal%20in%20the%20gastric%20antrum%20of%20wild-type%20and%20Ws/Ws%20rats&rft.jtitle=Anatomy%20and%20Embryology&rft.au=Mitsui,%20Retsu&rft.date=2003-05-01&rft.volume=206&rft.issue=6&rft.spage=453&rft.epage=460&rft.pages=453-460&rft.issn=0340-2061&rft.eissn=0340-2061&rft_id=info:doi/10.1007/s00429-003-0323-8&rft_dat=%3Cproquest_cross%3E2788904631%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1112073191&rft_id=info:pmid/12700899&rfr_iscdi=true |