Universal primers for amplification of the complete mitochondrial control region in marine fish species
Through multiple alignment analysis of mitochondrial tRNA-Thr and tRNA-Phe sequences from 161 fishes, new universal primers specially targeting the entire mitochondrial control region were designed. This new primer set successfully amplified the expected PCR products from various kinds of marine fis...
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| Veröffentlicht in: | Molecular biology (New York) 2012-09, Vol.46 (5), p.727-730 |
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| container_title | Molecular biology (New York) |
| container_volume | 46 |
| creator | Cheng, Y. -Z. Xu, T. -J. Jin, X. -X. Tang, D. Wei, T. Sun, Y. -Y. Meng, F. -Q. Shi, G. Wang, R. -X. |
| description | Through multiple alignment analysis of mitochondrial tRNA-Thr and tRNA-Phe sequences from 161 fishes, new universal primers specially targeting the entire mitochondrial control region were designed. This new primer set successfully amplified the expected PCR products from various kinds of marine fish species, belonging to various families, and the amplified segments were confirmed to be the control region by sequencing. These primers provide a useful tool to study the control region diversity in economically important fish species, the possible mechanism of control region evolution, and the functions of the conserved motifs in the control region. |
| doi_str_mv | 10.1134/S0026893312040024 |
| format | Article |
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This new primer set successfully amplified the expected PCR products from various kinds of marine fish species, belonging to various families, and the amplified segments were confirmed to be the control region by sequencing. These primers provide a useful tool to study the control region diversity in economically important fish species, the possible mechanism of control region evolution, and the functions of the conserved motifs in the control region.</description><identifier>ISSN: 0026-8933</identifier><identifier>EISSN: 1608-3245</identifier><identifier>DOI: 10.1134/S0026893312040024</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Fish ; Human Genetics ; Life Sciences ; Marine biology ; Mitochondrial DNA ; Molecular biology ; Polymerase chain reaction ; Short Communication</subject><ispartof>Molecular biology (New York), 2012-09, Vol.46 (5), p.727-730</ispartof><rights>Pleiades Publishing, Ltd. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-89316ae8e9416af7dde8b2a6323b9878f8d4c562ad358d3deed1814df59c56ea3</citedby><cites>FETCH-LOGICAL-c382t-89316ae8e9416af7dde8b2a6323b9878f8d4c562ad358d3deed1814df59c56ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0026893312040024$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0026893312040024$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Cheng, Y. -Z.</creatorcontrib><creatorcontrib>Xu, T. -J.</creatorcontrib><creatorcontrib>Jin, X. -X.</creatorcontrib><creatorcontrib>Tang, D.</creatorcontrib><creatorcontrib>Wei, T.</creatorcontrib><creatorcontrib>Sun, Y. -Y.</creatorcontrib><creatorcontrib>Meng, F. -Q.</creatorcontrib><creatorcontrib>Shi, G.</creatorcontrib><creatorcontrib>Wang, R. -X.</creatorcontrib><title>Universal primers for amplification of the complete mitochondrial control region in marine fish species</title><title>Molecular biology (New York)</title><addtitle>Mol Biol</addtitle><description>Through multiple alignment analysis of mitochondrial tRNA-Thr and tRNA-Phe sequences from 161 fishes, new universal primers specially targeting the entire mitochondrial control region were designed. This new primer set successfully amplified the expected PCR products from various kinds of marine fish species, belonging to various families, and the amplified segments were confirmed to be the control region by sequencing. These primers provide a useful tool to study the control region diversity in economically important fish species, the possible mechanism of control region evolution, and the functions of the conserved motifs in the control region.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Fish</subject><subject>Human Genetics</subject><subject>Life Sciences</subject><subject>Marine biology</subject><subject>Mitochondrial DNA</subject><subject>Molecular biology</subject><subject>Polymerase chain reaction</subject><subject>Short Communication</subject><issn>0026-8933</issn><issn>1608-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kE1LAzEQhoMoWKs_wFvA82q-dps9SvELCh605yVNJm3KbrImqeC_N0s9CCJMmGHmeWcyg9A1JbeUcnH3RghrZMs5ZUSUWJygGW2IrDgT9SmaTeVqqp-ji5T2hNBibIa2a-8-ISbV4zG6oUTYhojVMPbOOq2yCx4Hi_MOsA4lCxnw4HLQu-BNdEWng88x9DjCdoKdx4OKzgO2Lu1wGkE7SJfozKo-wdWPn6P148P78rlavT69LO9XleaS5emHtFEgoRXF24UxIDdMNZzxTSsX0kojdN0wZXgtDTcAhkoqjK3bkgbF5-jm2HeM4eMAKXf7cIi-jOwoaWvalkcLRY-UjiGlCLabllfxq0DddM_uzz2Lhh01qbB-C_F35_9E30IueL4</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Cheng, Y. -Z.</creator><creator>Xu, T. -J.</creator><creator>Jin, X. -X.</creator><creator>Tang, D.</creator><creator>Wei, T.</creator><creator>Sun, Y. -Y.</creator><creator>Meng, F. -Q.</creator><creator>Shi, G.</creator><creator>Wang, R. -X.</creator><general>SP MAIK Nauka/Interperiodica</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20120901</creationdate><title>Universal primers for amplification of the complete mitochondrial control region in marine fish species</title><author>Cheng, Y. -Z. ; Xu, T. -J. ; Jin, X. -X. ; Tang, D. ; Wei, T. ; Sun, Y. -Y. ; Meng, F. -Q. ; Shi, G. ; Wang, R. -X.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-89316ae8e9416af7dde8b2a6323b9878f8d4c562ad358d3deed1814df59c56ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Fish</topic><topic>Human Genetics</topic><topic>Life Sciences</topic><topic>Marine biology</topic><topic>Mitochondrial DNA</topic><topic>Molecular biology</topic><topic>Polymerase chain reaction</topic><topic>Short Communication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, Y. -Z.</creatorcontrib><creatorcontrib>Xu, T. -J.</creatorcontrib><creatorcontrib>Jin, X. -X.</creatorcontrib><creatorcontrib>Tang, D.</creatorcontrib><creatorcontrib>Wei, T.</creatorcontrib><creatorcontrib>Sun, Y. -Y.</creatorcontrib><creatorcontrib>Meng, F. -Q.</creatorcontrib><creatorcontrib>Shi, G.</creatorcontrib><creatorcontrib>Wang, R. -X.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Molecular biology (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Y. -Z.</au><au>Xu, T. -J.</au><au>Jin, X. -X.</au><au>Tang, D.</au><au>Wei, T.</au><au>Sun, Y. -Y.</au><au>Meng, F. -Q.</au><au>Shi, G.</au><au>Wang, R. -X.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Universal primers for amplification of the complete mitochondrial control region in marine fish species</atitle><jtitle>Molecular biology (New York)</jtitle><stitle>Mol Biol</stitle><date>2012-09-01</date><risdate>2012</risdate><volume>46</volume><issue>5</issue><spage>727</spage><epage>730</epage><pages>727-730</pages><issn>0026-8933</issn><eissn>1608-3245</eissn><abstract>Through multiple alignment analysis of mitochondrial tRNA-Thr and tRNA-Phe sequences from 161 fishes, new universal primers specially targeting the entire mitochondrial control region were designed. 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| ispartof | Molecular biology (New York), 2012-09, Vol.46 (5), p.727-730 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Biochemistry Biomedical and Life Sciences Fish Human Genetics Life Sciences Marine biology Mitochondrial DNA Molecular biology Polymerase chain reaction Short Communication |
| title | Universal primers for amplification of the complete mitochondrial control region in marine fish species |
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