Peroxisomal Localization of CuZn Superoxide Dismutase in the Male Reproductive Tissues of the Olive Tree

Superoxide dismutases (SODs) are a class of antioxidant enzymes which catalyze the dismutation of superoxide into oxygen and hydrogen peroxide, therefore controlling cellular levels of Reactive Oxygen Species (ROS). In the mature pollen grains of the olive tree, the presence of several forms of CuZn...

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Veröffentlicht in:Microscopy and microanalysis 2012-08, Vol.18 (S5), p.33-34
Hauptverfasser: Zafra, A., Jiménez-Quesada, M. J., Traverso, J. A., Corpas, F. J., Rodríguez-García, M. I., Alché, J. D.
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container_end_page 34
container_issue S5
container_start_page 33
container_title Microscopy and microanalysis
container_volume 18
creator Zafra, A.
Jiménez-Quesada, M. J.
Traverso, J. A.
Corpas, F. J.
Rodríguez-García, M. I.
Alché, J. D.
description Superoxide dismutases (SODs) are a class of antioxidant enzymes which catalyze the dismutation of superoxide into oxygen and hydrogen peroxide, therefore controlling cellular levels of Reactive Oxygen Species (ROS). In the mature pollen grains of the olive tree, the presence of several forms of CuZn-SOD and the cytosolic localization of the enzyme have been described. The present study was aimed to elucidate the adaptation of the oxidative metabolism to the changing conditions occurring during the course of olive pollen formation, hydration and pollen tube emergence and growth. We used a polyclonal antibody (raised against a KLH-linked synthetic peptide including a consensus sequence for CuZn-SODs in olive pollen) in immunocytochemical experiments carried out by Fluorescence (FM) and Transmission Electron Microcopy (TEM).
doi_str_mv 10.1017/S1431927612012822
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source Alma/SFX Local Collection
subjects Fruit trees
Hydrogen peroxide
Life Sciences
Pollen
title Peroxisomal Localization of CuZn Superoxide Dismutase in the Male Reproductive Tissues of the Olive Tree
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