Chemopreventive effects of in vitro digested and fermented bread in human colon cells
Purpose Bread as a staple food product represents an important source for dietary fibre consumption. Effects of wheat bread, wholemeal wheat bread and wholemeal rye bread on mechanisms which could have impact on chemoprevention were analysed in colon cells after in vitro fermentation. Methods Effect...
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| Veröffentlicht in: | European journal of nutrition 2012-10, Vol.51 (7), p.827-839 |
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| container_title | European journal of nutrition |
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| creator | Schlörmann, Wiebke Hiller, Beate Jahns, Franziska Zöger, Romy Hennemeier, Isabell Wilhelm, Anne Lindhauer, Meinolf G. Glei, Michael |
| description | Purpose
Bread as a staple food product represents an important source for dietary fibre consumption. Effects of wheat bread, wholemeal wheat bread and wholemeal rye bread on mechanisms which could have impact on chemoprevention were analysed in colon cells after in vitro fermentation.
Methods
Effects of fermented bread samples on gene expression, glutathione S-transferase activity and glutathione content, differentiation, growth and apoptosis were investigated using the human colon adenoma cell line LT97. Additionally, apoptosis was studied in normal and tumour colon tissue by determination of caspase activities.
Results
The expression of 76 genes (biotransformation, differentiation, apoptosis) was significantly upregulated (1.5-fold) in LT97 cells. The fermented bread samples were able to significantly increase glutathione S-transferase activity (1.8-fold) and glutathione content (1.4-fold) of the cells. Alkaline phosphatase activity as a marker of differentiation was also significantly enhanced (1.7-fold). The fermented bread samples significantly inhibited LT97 cell growth and increased the level of apoptotic cells (1.8-fold). Only marginal effects on apoptosis in tumour compared to normal tissue were observed.
Conclusions
This is the first study which presents chemopreventive effects of different breads after in vitro fermentation. In spite of differences in composition, the results were comparable between the bread types. Nevertheless, they indicate a potential involvement of this staple food product regarding the prevention of colon cancer. |
| doi_str_mv | 10.1007/s00394-011-0262-8 |
| format | Article |
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Bread as a staple food product represents an important source for dietary fibre consumption. Effects of wheat bread, wholemeal wheat bread and wholemeal rye bread on mechanisms which could have impact on chemoprevention were analysed in colon cells after in vitro fermentation.
Methods
Effects of fermented bread samples on gene expression, glutathione S-transferase activity and glutathione content, differentiation, growth and apoptosis were investigated using the human colon adenoma cell line LT97. Additionally, apoptosis was studied in normal and tumour colon tissue by determination of caspase activities.
Results
The expression of 76 genes (biotransformation, differentiation, apoptosis) was significantly upregulated (1.5-fold) in LT97 cells. The fermented bread samples were able to significantly increase glutathione S-transferase activity (1.8-fold) and glutathione content (1.4-fold) of the cells. Alkaline phosphatase activity as a marker of differentiation was also significantly enhanced (1.7-fold). The fermented bread samples significantly inhibited LT97 cell growth and increased the level of apoptotic cells (1.8-fold). Only marginal effects on apoptosis in tumour compared to normal tissue were observed.
Conclusions
This is the first study which presents chemopreventive effects of different breads after in vitro fermentation. In spite of differences in composition, the results were comparable between the bread types. Nevertheless, they indicate a potential involvement of this staple food product regarding the prevention of colon cancer.</description><identifier>ISSN: 1436-6207</identifier><identifier>EISSN: 1436-6215</identifier><identifier>DOI: 10.1007/s00394-011-0262-8</identifier><identifier>PMID: 22033853</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Alkaline Phosphatase - metabolism ; Apoptosis - drug effects ; Biological and medical sciences ; Bread ; Cell Differentiation - drug effects ; Cell Line, Tumor ; Chemistry ; Chemistry and Materials Science ; Chemoprevention - methods ; Colon - cytology ; Colonic Neoplasms - pathology ; Feeding. Feeding behavior ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Glutathione - metabolism ; Glutathione Transferase - metabolism ; Humans ; Microarray Analysis ; Nutrition ; Original Contribution ; RNA, Neoplasm - genetics ; Secale - chemistry ; Triticum - chemistry ; Up-Regulation ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>European journal of nutrition, 2012-10, Vol.51 (7), p.827-839</ispartof><rights>Springer-Verlag 2011</rights><rights>2015 INIST-CNRS</rights><rights>Springer-Verlag Berlin Heidelberg 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-e39ba24b42ebf62b21f53a564308fd6341e3a72e140fb13ce00122daac93b0c73</citedby><cites>FETCH-LOGICAL-c402t-e39ba24b42ebf62b21f53a564308fd6341e3a72e140fb13ce00122daac93b0c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00394-011-0262-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00394-011-0262-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26389041$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22033853$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schlörmann, Wiebke</creatorcontrib><creatorcontrib>Hiller, Beate</creatorcontrib><creatorcontrib>Jahns, Franziska</creatorcontrib><creatorcontrib>Zöger, Romy</creatorcontrib><creatorcontrib>Hennemeier, Isabell</creatorcontrib><creatorcontrib>Wilhelm, Anne</creatorcontrib><creatorcontrib>Lindhauer, Meinolf G.</creatorcontrib><creatorcontrib>Glei, Michael</creatorcontrib><title>Chemopreventive effects of in vitro digested and fermented bread in human colon cells</title><title>European journal of nutrition</title><addtitle>Eur J Nutr</addtitle><addtitle>Eur J Nutr</addtitle><description>Purpose
Bread as a staple food product represents an important source for dietary fibre consumption. Effects of wheat bread, wholemeal wheat bread and wholemeal rye bread on mechanisms which could have impact on chemoprevention were analysed in colon cells after in vitro fermentation.
Methods
Effects of fermented bread samples on gene expression, glutathione S-transferase activity and glutathione content, differentiation, growth and apoptosis were investigated using the human colon adenoma cell line LT97. Additionally, apoptosis was studied in normal and tumour colon tissue by determination of caspase activities.
Results
The expression of 76 genes (biotransformation, differentiation, apoptosis) was significantly upregulated (1.5-fold) in LT97 cells. The fermented bread samples were able to significantly increase glutathione S-transferase activity (1.8-fold) and glutathione content (1.4-fold) of the cells. Alkaline phosphatase activity as a marker of differentiation was also significantly enhanced (1.7-fold). The fermented bread samples significantly inhibited LT97 cell growth and increased the level of apoptotic cells (1.8-fold). Only marginal effects on apoptosis in tumour compared to normal tissue were observed.
Conclusions
This is the first study which presents chemopreventive effects of different breads after in vitro fermentation. In spite of differences in composition, the results were comparable between the bread types. Nevertheless, they indicate a potential involvement of this staple food product regarding the prevention of colon cancer.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Bread</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemoprevention - methods</subject><subject>Colon - cytology</subject><subject>Colonic Neoplasms - pathology</subject><subject>Feeding. Feeding behavior</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutathione - metabolism</subject><subject>Glutathione Transferase - metabolism</subject><subject>Humans</subject><subject>Microarray Analysis</subject><subject>Nutrition</subject><subject>Original Contribution</subject><subject>RNA, Neoplasm - genetics</subject><subject>Secale - chemistry</subject><subject>Triticum - chemistry</subject><subject>Up-Regulation</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>1436-6207</issn><issn>1436-6215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kMlOwzAQQC0EoqXwAVyQJcQxMF7iJEdUsUmVuNBz5DjjNlWWYqeV-HscpRQuXDy25s3iR8g1g3sGkDx4AJHJCBiLgCsepSdkyqRQkeIsPj3eIZmQC-83AMCFYudkwjkIkcZiSpbzNTbd1uEe277aI0Vr0fSedpZWLd1XvetoWa3Q91hS3ZbUomsCG16FQ10O1HrX6Jaaru7CiXXtL8mZ1bXHq0OckeXz08f8NVq8v7zNHxeRkcD7CEVWaC4LybGwihec2VjoWEkBqS2VkAyFTjgyCbZgwiAA47zU2mSiAJOIGbkd-25d97kLO-abbufaMDJnoDIRPpwNFBsp4zrvHdp866pGu68A5YPIfBSZB5H5IDJPQ83NofOuaLA8VvyYC8DdAdDe6No63ZrK_3JKpBlIFjg-cj6k2hW6vyv-N_0bbiWKHQ</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Schlörmann, Wiebke</creator><creator>Hiller, Beate</creator><creator>Jahns, Franziska</creator><creator>Zöger, Romy</creator><creator>Hennemeier, Isabell</creator><creator>Wilhelm, Anne</creator><creator>Lindhauer, Meinolf G.</creator><creator>Glei, Michael</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RQ</scope><scope>7RV</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>20121001</creationdate><title>Chemopreventive effects of in vitro digested and fermented bread in human colon cells</title><author>Schlörmann, Wiebke ; Hiller, Beate ; Jahns, Franziska ; Zöger, Romy ; Hennemeier, Isabell ; Wilhelm, Anne ; Lindhauer, Meinolf G. ; Glei, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-e39ba24b42ebf62b21f53a564308fd6341e3a72e140fb13ce00122daac93b0c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Bread</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemoprevention - methods</topic><topic>Colon - cytology</topic><topic>Colonic Neoplasms - pathology</topic><topic>Feeding. Feeding behavior</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutathione - metabolism</topic><topic>Glutathione Transferase - metabolism</topic><topic>Humans</topic><topic>Microarray Analysis</topic><topic>Nutrition</topic><topic>Original Contribution</topic><topic>RNA, Neoplasm - genetics</topic><topic>Secale - chemistry</topic><topic>Triticum - chemistry</topic><topic>Up-Regulation</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schlörmann, Wiebke</creatorcontrib><creatorcontrib>Hiller, Beate</creatorcontrib><creatorcontrib>Jahns, Franziska</creatorcontrib><creatorcontrib>Zöger, Romy</creatorcontrib><creatorcontrib>Hennemeier, Isabell</creatorcontrib><creatorcontrib>Wilhelm, Anne</creatorcontrib><creatorcontrib>Lindhauer, Meinolf G.</creatorcontrib><creatorcontrib>Glei, Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Career & Technical Education Database</collection><collection>Nursing & Allied Health Database</collection><collection>Physical Education Index</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>European journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schlörmann, Wiebke</au><au>Hiller, Beate</au><au>Jahns, Franziska</au><au>Zöger, Romy</au><au>Hennemeier, Isabell</au><au>Wilhelm, Anne</au><au>Lindhauer, Meinolf G.</au><au>Glei, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemopreventive effects of in vitro digested and fermented bread in human colon cells</atitle><jtitle>European journal of nutrition</jtitle><stitle>Eur J Nutr</stitle><addtitle>Eur J Nutr</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>51</volume><issue>7</issue><spage>827</spage><epage>839</epage><pages>827-839</pages><issn>1436-6207</issn><eissn>1436-6215</eissn><abstract>Purpose
Bread as a staple food product represents an important source for dietary fibre consumption. Effects of wheat bread, wholemeal wheat bread and wholemeal rye bread on mechanisms which could have impact on chemoprevention were analysed in colon cells after in vitro fermentation.
Methods
Effects of fermented bread samples on gene expression, glutathione S-transferase activity and glutathione content, differentiation, growth and apoptosis were investigated using the human colon adenoma cell line LT97. Additionally, apoptosis was studied in normal and tumour colon tissue by determination of caspase activities.
Results
The expression of 76 genes (biotransformation, differentiation, apoptosis) was significantly upregulated (1.5-fold) in LT97 cells. The fermented bread samples were able to significantly increase glutathione S-transferase activity (1.8-fold) and glutathione content (1.4-fold) of the cells. Alkaline phosphatase activity as a marker of differentiation was also significantly enhanced (1.7-fold). The fermented bread samples significantly inhibited LT97 cell growth and increased the level of apoptotic cells (1.8-fold). Only marginal effects on apoptosis in tumour compared to normal tissue were observed.
Conclusions
This is the first study which presents chemopreventive effects of different breads after in vitro fermentation. In spite of differences in composition, the results were comparable between the bread types. Nevertheless, they indicate a potential involvement of this staple food product regarding the prevention of colon cancer.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>22033853</pmid><doi>10.1007/s00394-011-0262-8</doi><tpages>13</tpages></addata></record> |
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| subjects | Alkaline Phosphatase - metabolism Apoptosis - drug effects Biological and medical sciences Bread Cell Differentiation - drug effects Cell Line, Tumor Chemistry Chemistry and Materials Science Chemoprevention - methods Colon - cytology Colonic Neoplasms - pathology Feeding. Feeding behavior Fermentation Fundamental and applied biological sciences. Psychology Glutathione - metabolism Glutathione Transferase - metabolism Humans Microarray Analysis Nutrition Original Contribution RNA, Neoplasm - genetics Secale - chemistry Triticum - chemistry Up-Regulation Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Chemopreventive effects of in vitro digested and fermented bread in human colon cells |
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