Cecal Ligation and Puncture as a Model of Sepsis in the Rat: Influence of the Puncture Size on Mortality, Bacteremia, Endotoxemia and Tumor Necrosis Factor Alpha Levels
Background: Cecal ligation and puncture is a widely used experimental model of sepsis. Aim of the Study: The present study was aimed to evaluate the influence of the size of the cecal puncture on mortality, bacteremia, endotoxemia and plasma TNF-α levels. Materials and Methods: Female Sprague-Dawley...
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| Veröffentlicht in: | European surgical research 2001, Vol.33 (2), p.77-79 |
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| creator | Otero-Antón, E. González-Quintela, A. López-Soto, A. López-Ben, S. Llovo, J. Pérez, L.F. |
| description | Background: Cecal ligation and puncture is a widely used experimental model of sepsis. Aim of the Study: The present study was aimed to evaluate the influence of the size of the cecal puncture on mortality, bacteremia, endotoxemia and plasma TNF-α levels. Materials and Methods: Female Sprague-Dawley rats underwent cecal ligation and puncture, divided into the following groups, defined by the diameter of the cecal puncture: 0.5-cm blade incision (n = 25), 13-gauge (n = 25), 16-gauge (n = 25), 18-gauge puncture (n = 25) and 4 punctures with a 22-gauge needle (n = 25). A sham operation was performed in another 25 rats. Three animals of each group were sacrificed 5 h after the procedure for blood cultures as well as determination of plasma endotoxin and TNF-α. The remaining animals were followed up for a week after cecal ligation and puncture for evaluation of mortality. Results: Five hours after cecal ligation and puncture, bacteremia was present in all animals, independently of the puncture size. Endotoxemia and plasma TNF levels tended to increase along with the diameter of the cecal puncture. Mortality gradually increased with the puncture size, from 27% with a 22-gauge needle to 95% with the blade incision. Conclusions: The severity of sepsis obtained with cecal ligation and puncture in rats can be easily modulated varying the size of the puncture. |
| doi_str_mv | 10.1159/000049698 |
| format | Article |
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Aim of the Study: The present study was aimed to evaluate the influence of the size of the cecal puncture on mortality, bacteremia, endotoxemia and plasma TNF-α levels. Materials and Methods: Female Sprague-Dawley rats underwent cecal ligation and puncture, divided into the following groups, defined by the diameter of the cecal puncture: 0.5-cm blade incision (n = 25), 13-gauge (n = 25), 16-gauge (n = 25), 18-gauge puncture (n = 25) and 4 punctures with a 22-gauge needle (n = 25). A sham operation was performed in another 25 rats. Three animals of each group were sacrificed 5 h after the procedure for blood cultures as well as determination of plasma endotoxin and TNF-α. The remaining animals were followed up for a week after cecal ligation and puncture for evaluation of mortality. Results: Five hours after cecal ligation and puncture, bacteremia was present in all animals, independently of the puncture size. Endotoxemia and plasma TNF levels tended to increase along with the diameter of the cecal puncture. Mortality gradually increased with the puncture size, from 27% with a 22-gauge needle to 95% with the blade incision. Conclusions: The severity of sepsis obtained with cecal ligation and puncture in rats can be easily modulated varying the size of the puncture.</description><identifier>ISSN: 0014-312X</identifier><identifier>EISSN: 1421-9921</identifier><identifier>DOI: 10.1159/000049698</identifier><identifier>PMID: 11399872</identifier><identifier>CODEN: EUSRBM</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Bacteremia - complications ; Bacteroides Infections - etiology ; Biological and medical sciences ; Cecum ; Disease Models, Animal ; Emergency and intensive care: infection, septic shock ; Endotoxemia - complications ; Gram-Negative Bacterial Infections - etiology ; Infection - blood ; Infection - etiology ; Infection - mortality ; Intensive care medicine ; Ligation ; Medical sciences ; Original Paper ; Peritonitis - microbiology ; Punctures ; Rats ; Rats, Sprague-Dawley ; Survival Analysis ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>European surgical research, 2001, Vol.33 (2), p.77-79</ispartof><rights>2001 S. Karger AG, Basel</rights><rights>2001 INIST-CNRS</rights><rights>Copyright 2001 S. Karger AG, Basel</rights><rights>Copyright (c) 2001 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-2919ddf5b16a8d5b195f755b7b56ebfc789bfdeda892cc13e3391d36dd49cfa43</citedby><cites>FETCH-LOGICAL-c450t-2919ddf5b16a8d5b195f755b7b56ebfc789bfdeda892cc13e3391d36dd49cfa43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2423,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=986689$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11399872$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Otero-Antón, E.</creatorcontrib><creatorcontrib>González-Quintela, A.</creatorcontrib><creatorcontrib>López-Soto, A.</creatorcontrib><creatorcontrib>López-Ben, S.</creatorcontrib><creatorcontrib>Llovo, J.</creatorcontrib><creatorcontrib>Pérez, L.F.</creatorcontrib><title>Cecal Ligation and Puncture as a Model of Sepsis in the Rat: Influence of the Puncture Size on Mortality, Bacteremia, Endotoxemia and Tumor Necrosis Factor Alpha Levels</title><title>European surgical research</title><addtitle>Eur Surg Res</addtitle><description>Background: Cecal ligation and puncture is a widely used experimental model of sepsis. Aim of the Study: The present study was aimed to evaluate the influence of the size of the cecal puncture on mortality, bacteremia, endotoxemia and plasma TNF-α levels. Materials and Methods: Female Sprague-Dawley rats underwent cecal ligation and puncture, divided into the following groups, defined by the diameter of the cecal puncture: 0.5-cm blade incision (n = 25), 13-gauge (n = 25), 16-gauge (n = 25), 18-gauge puncture (n = 25) and 4 punctures with a 22-gauge needle (n = 25). A sham operation was performed in another 25 rats. Three animals of each group were sacrificed 5 h after the procedure for blood cultures as well as determination of plasma endotoxin and TNF-α. The remaining animals were followed up for a week after cecal ligation and puncture for evaluation of mortality. Results: Five hours after cecal ligation and puncture, bacteremia was present in all animals, independently of the puncture size. Endotoxemia and plasma TNF levels tended to increase along with the diameter of the cecal puncture. Mortality gradually increased with the puncture size, from 27% with a 22-gauge needle to 95% with the blade incision. Conclusions: The severity of sepsis obtained with cecal ligation and puncture in rats can be easily modulated varying the size of the puncture.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Bacteremia - complications</subject><subject>Bacteroides Infections - etiology</subject><subject>Biological and medical sciences</subject><subject>Cecum</subject><subject>Disease Models, Animal</subject><subject>Emergency and intensive care: infection, septic shock</subject><subject>Endotoxemia - complications</subject><subject>Gram-Negative Bacterial Infections - etiology</subject><subject>Infection - blood</subject><subject>Infection - etiology</subject><subject>Infection - mortality</subject><subject>Intensive care medicine</subject><subject>Ligation</subject><subject>Medical sciences</subject><subject>Original Paper</subject><subject>Peritonitis - microbiology</subject><subject>Punctures</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Survival Analysis</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>0014-312X</issn><issn>1421-9921</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkd9rFDEQx4Mo9qw--CxIUBCErm729_hWj6sWzh_0Kvi2ZJNJm7qbrElWrH-Rf6bZ3nmKmJdhZj4z3yRfQh6y9AVjJbxM4ymgguYWWbAiYwlAxm6TRZqyIslZ9vmA3PP-KqYl1HCXHDCWAzR1tiA_lyh4T9f6ggdtDeVG0o-TEWFySLmnnL6zEntqFd3g6LWn2tBwifSMh1f01Kh-QiNw7s_V_ehG_4hFE6dd4L0O10f0NRcBHQ6aH9GVkTbY73NyI3k-DdbR9yicnTVOIhrz43685HSN37D398kdxXuPD3bxkHw6WZ0v3ybrD29Ol8frRBRlGpIMGEipyo5VvJExQKnqsuzqrqywU6JuoFMSJW8gE4LlmOfAZF5JWYBQvMgPybPt3tHZrxP60A7aC-x7btBOvq1TyOJnz-DTf8ArOzkT79aytIIsZ2lTRur5lppf5h2qdnR64O46Qu1sXrs3L7KPdxunbkD5h9y59Zck99E15bgR2u85aKqqgUg92lJfuLtAt2__Fnny3-5qc3YDtKNU-S8j3bZg</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Otero-Antón, E.</creator><creator>González-Quintela, A.</creator><creator>López-Soto, A.</creator><creator>López-Ben, S.</creator><creator>Llovo, J.</creator><creator>Pérez, L.F.</creator><general>Karger</general><general>S. 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Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Bacteremia - complications</topic><topic>Bacteroides Infections - etiology</topic><topic>Biological and medical sciences</topic><topic>Cecum</topic><topic>Disease Models, Animal</topic><topic>Emergency and intensive care: infection, septic shock</topic><topic>Endotoxemia - complications</topic><topic>Gram-Negative Bacterial Infections - etiology</topic><topic>Infection - blood</topic><topic>Infection - etiology</topic><topic>Infection - mortality</topic><topic>Intensive care medicine</topic><topic>Ligation</topic><topic>Medical sciences</topic><topic>Original Paper</topic><topic>Peritonitis - microbiology</topic><topic>Punctures</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Survival Analysis</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Otero-Antón, E.</creatorcontrib><creatorcontrib>González-Quintela, A.</creatorcontrib><creatorcontrib>López-Soto, A.</creatorcontrib><creatorcontrib>López-Ben, S.</creatorcontrib><creatorcontrib>Llovo, J.</creatorcontrib><creatorcontrib>Pérez, L.F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>European surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Otero-Antón, E.</au><au>González-Quintela, A.</au><au>López-Soto, A.</au><au>López-Ben, S.</au><au>Llovo, J.</au><au>Pérez, L.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cecal Ligation and Puncture as a Model of Sepsis in the Rat: Influence of the Puncture Size on Mortality, Bacteremia, Endotoxemia and Tumor Necrosis Factor Alpha Levels</atitle><jtitle>European surgical research</jtitle><addtitle>Eur Surg Res</addtitle><date>2001</date><risdate>2001</risdate><volume>33</volume><issue>2</issue><spage>77</spage><epage>79</epage><pages>77-79</pages><issn>0014-312X</issn><eissn>1421-9921</eissn><coden>EUSRBM</coden><abstract>Background: Cecal ligation and puncture is a widely used experimental model of sepsis. Aim of the Study: The present study was aimed to evaluate the influence of the size of the cecal puncture on mortality, bacteremia, endotoxemia and plasma TNF-α levels. Materials and Methods: Female Sprague-Dawley rats underwent cecal ligation and puncture, divided into the following groups, defined by the diameter of the cecal puncture: 0.5-cm blade incision (n = 25), 13-gauge (n = 25), 16-gauge (n = 25), 18-gauge puncture (n = 25) and 4 punctures with a 22-gauge needle (n = 25). A sham operation was performed in another 25 rats. Three animals of each group were sacrificed 5 h after the procedure for blood cultures as well as determination of plasma endotoxin and TNF-α. The remaining animals were followed up for a week after cecal ligation and puncture for evaluation of mortality. Results: Five hours after cecal ligation and puncture, bacteremia was present in all animals, independently of the puncture size. Endotoxemia and plasma TNF levels tended to increase along with the diameter of the cecal puncture. Mortality gradually increased with the puncture size, from 27% with a 22-gauge needle to 95% with the blade incision. Conclusions: The severity of sepsis obtained with cecal ligation and puncture in rats can be easily modulated varying the size of the puncture.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>11399872</pmid><doi>10.1159/000049698</doi><tpages>3</tpages></addata></record> |
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| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Bacteremia - complications Bacteroides Infections - etiology Biological and medical sciences Cecum Disease Models, Animal Emergency and intensive care: infection, septic shock Endotoxemia - complications Gram-Negative Bacterial Infections - etiology Infection - blood Infection - etiology Infection - mortality Intensive care medicine Ligation Medical sciences Original Paper Peritonitis - microbiology Punctures Rats Rats, Sprague-Dawley Survival Analysis Tumor Necrosis Factor-alpha - metabolism |
| title | Cecal Ligation and Puncture as a Model of Sepsis in the Rat: Influence of the Puncture Size on Mortality, Bacteremia, Endotoxemia and Tumor Necrosis Factor Alpha Levels |
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