Decreased Tubular Uptake of L-3,4-Dihydroxyphenylalanine in Streptozotocin-Induced Diabetic Rats
Background/Aims: This study determined alterations in renal dopamine production in streptozotocin-treated rats and explored the mechanisms underlying this alteration. Methods: Streptozotocin (65 mg/kg) or vehicle was administered to 3-month-old male Wistar rats. Treated animals had hyperglycemia, gl...
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| Veröffentlicht in: | Hormone research 2001-01, Vol.55 (6), p.282-287 |
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| creator | Carranza, Andrea Karabatas, Liliana Barontini, Marta Armando, Ines |
| description | Background/Aims: This study determined alterations in renal dopamine production in streptozotocin-treated rats and explored the mechanisms underlying this alteration. Methods: Streptozotocin (65 mg/kg) or vehicle was administered to 3-month-old male Wistar rats. Treated animals had hyperglycemia, glycosuria and increased diuresis, natriuresis and excretion of L-dopa. Urinary dopamine and dihydroxyphenylacetic acid were similar to those in control animals. The production of dopamine by renal cortex slices from treated rats was significantly less than that from control animals. The addition of glucose (8.4–18.4 mM) to the incubation medium decreased about 40% the uptake of L-dopa by isolated proximal tubular cells. Scatchard analysis of the saturation curves obtained in this condition showed a decrease in the V max without changes in the K m . Results: Our results confirm previous studies suggesting a renal dopaminergic deficiency in insulin-dependent diabetes and provide evidence strongly suggesting that a decrease in the number of tubular L-dopa transport sites is the underlying defect of this deficiency. Conclusion: These results highlight the role of the uptake of dopa as an important modulator of renal dopamine synthesis. |
| doi_str_mv | 10.1159/000050014 |
| format | Article |
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Methods: Streptozotocin (65 mg/kg) or vehicle was administered to 3-month-old male Wistar rats. Treated animals had hyperglycemia, glycosuria and increased diuresis, natriuresis and excretion of L-dopa. Urinary dopamine and dihydroxyphenylacetic acid were similar to those in control animals. The production of dopamine by renal cortex slices from treated rats was significantly less than that from control animals. The addition of glucose (8.4–18.4 mM) to the incubation medium decreased about 40% the uptake of L-dopa by isolated proximal tubular cells. Scatchard analysis of the saturation curves obtained in this condition showed a decrease in the V max without changes in the K m . Results: Our results confirm previous studies suggesting a renal dopaminergic deficiency in insulin-dependent diabetes and provide evidence strongly suggesting that a decrease in the number of tubular L-dopa transport sites is the underlying defect of this deficiency. Conclusion: These results highlight the role of the uptake of dopa as an important modulator of renal dopamine synthesis.</description><identifier>ISSN: 1663-2818</identifier><identifier>ISSN: 0301-0163</identifier><identifier>EISSN: 1663-2826</identifier><identifier>DOI: 10.1159/000050014</identifier><identifier>PMID: 11805432</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>3,4-Dihydroxyphenylacetic Acid - urine ; Animals ; Biological Transport ; Diabetes Mellitus, Experimental - metabolism ; Dopamine - deficiency ; Dopamine - metabolism ; Dose-Response Relationship, Drug ; Glucose - pharmacology ; In Vitro Techniques ; Kidney - metabolism ; Kidney Tubules, Proximal - metabolism ; Levodopa - pharmacokinetics ; Levodopa - pharmacology ; Levodopa - urine ; Male ; Original Paper ; Osmolar Concentration ; Rats ; Rats, Wistar</subject><ispartof>Hormone research, 2001-01, Vol.55 (6), p.282-287</ispartof><rights>2002 S. Karger AG, Basel</rights><rights>Copyright 2002 S. Karger AG, Basel</rights><rights>Copyright (c) 2001 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-8b82f673bcc62630f6228c4bc27c77857c463fd6668ef8ea427a3a940001222a3</citedby><cites>FETCH-LOGICAL-c356t-8b82f673bcc62630f6228c4bc27c77857c463fd6668ef8ea427a3a940001222a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2429,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11805432$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carranza, Andrea</creatorcontrib><creatorcontrib>Karabatas, Liliana</creatorcontrib><creatorcontrib>Barontini, Marta</creatorcontrib><creatorcontrib>Armando, Ines</creatorcontrib><title>Decreased Tubular Uptake of L-3,4-Dihydroxyphenylalanine in Streptozotocin-Induced Diabetic Rats</title><title>Hormone research</title><addtitle>Horm Res Paediatr</addtitle><description>Background/Aims: This study determined alterations in renal dopamine production in streptozotocin-treated rats and explored the mechanisms underlying this alteration. Methods: Streptozotocin (65 mg/kg) or vehicle was administered to 3-month-old male Wistar rats. Treated animals had hyperglycemia, glycosuria and increased diuresis, natriuresis and excretion of L-dopa. Urinary dopamine and dihydroxyphenylacetic acid were similar to those in control animals. The production of dopamine by renal cortex slices from treated rats was significantly less than that from control animals. The addition of glucose (8.4–18.4 mM) to the incubation medium decreased about 40% the uptake of L-dopa by isolated proximal tubular cells. Scatchard analysis of the saturation curves obtained in this condition showed a decrease in the V max without changes in the K m . Results: Our results confirm previous studies suggesting a renal dopaminergic deficiency in insulin-dependent diabetes and provide evidence strongly suggesting that a decrease in the number of tubular L-dopa transport sites is the underlying defect of this deficiency. Conclusion: These results highlight the role of the uptake of dopa as an important modulator of renal dopamine synthesis.</description><subject>3,4-Dihydroxyphenylacetic Acid - urine</subject><subject>Animals</subject><subject>Biological Transport</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Dopamine - deficiency</subject><subject>Dopamine - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Glucose - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Kidney - metabolism</subject><subject>Kidney Tubules, Proximal - metabolism</subject><subject>Levodopa - pharmacokinetics</subject><subject>Levodopa - pharmacology</subject><subject>Levodopa - urine</subject><subject>Male</subject><subject>Original Paper</subject><subject>Osmolar Concentration</subject><subject>Rats</subject><subject>Rats, Wistar</subject><issn>1663-2818</issn><issn>0301-0163</issn><issn>1663-2826</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpt0M1LwzAYBvAgihPdwbMgRUEQrDYfTdKjuOkGA2Fu55qmb7XaNTVpwfnXm7kxQcwlOfx48r4PQsc4usY4Tm4if-IowmwHHWDOaUgk4bvbN5Y91HfubcWoFAkW-6iHsYxiRskBeh6AtqAc5MGsy7pK2WDetOodAlMEk5BesXBQvi5zaz6XzSvUy0pVqi5rCMo6eGotNK35Mq3RZR2O67zTPmhQqgzaUgdT1bojtFeoykF_cx-i-f1wdjcKJ48P47vbSahpzNtQZpIUXNBMa044jQpOiNQs00RoIWQsNOO0yDnnEgoJihGhqEqY3woTQhQ9RBfr3Maajw5cmy5Kp6Hy04LpXCoIixIupYfnf-Cb6WztZ0txxBNCEk5X6nKttDXOWSjSxpYLZZcepave023v3p5uErtsAfmv3LTswdkavCv7AnYLRtPhT0La5IVHJ_-i9R_fi3aPYg</recordid><startdate>20010101</startdate><enddate>20010101</enddate><creator>Carranza, Andrea</creator><creator>Karabatas, Liliana</creator><creator>Barontini, Marta</creator><creator>Armando, Ines</creator><general>S. 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Barontini, Marta ; Armando, Ines</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-8b82f673bcc62630f6228c4bc27c77857c463fd6668ef8ea427a3a940001222a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>3,4-Dihydroxyphenylacetic Acid - urine</topic><topic>Animals</topic><topic>Biological Transport</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Dopamine - deficiency</topic><topic>Dopamine - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Glucose - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Kidney - metabolism</topic><topic>Kidney Tubules, Proximal - metabolism</topic><topic>Levodopa - pharmacokinetics</topic><topic>Levodopa - pharmacology</topic><topic>Levodopa - urine</topic><topic>Male</topic><topic>Original Paper</topic><topic>Osmolar Concentration</topic><topic>Rats</topic><topic>Rats, Wistar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carranza, Andrea</creatorcontrib><creatorcontrib>Karabatas, Liliana</creatorcontrib><creatorcontrib>Barontini, Marta</creatorcontrib><creatorcontrib>Armando, Ines</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Hormone research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carranza, Andrea</au><au>Karabatas, Liliana</au><au>Barontini, Marta</au><au>Armando, Ines</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased Tubular Uptake of L-3,4-Dihydroxyphenylalanine in Streptozotocin-Induced Diabetic Rats</atitle><jtitle>Hormone research</jtitle><addtitle>Horm Res Paediatr</addtitle><date>2001-01-01</date><risdate>2001</risdate><volume>55</volume><issue>6</issue><spage>282</spage><epage>287</epage><pages>282-287</pages><issn>1663-2818</issn><issn>0301-0163</issn><eissn>1663-2826</eissn><abstract>Background/Aims: This study determined alterations in renal dopamine production in streptozotocin-treated rats and explored the mechanisms underlying this alteration. Methods: Streptozotocin (65 mg/kg) or vehicle was administered to 3-month-old male Wistar rats. Treated animals had hyperglycemia, glycosuria and increased diuresis, natriuresis and excretion of L-dopa. Urinary dopamine and dihydroxyphenylacetic acid were similar to those in control animals. The production of dopamine by renal cortex slices from treated rats was significantly less than that from control animals. The addition of glucose (8.4–18.4 mM) to the incubation medium decreased about 40% the uptake of L-dopa by isolated proximal tubular cells. Scatchard analysis of the saturation curves obtained in this condition showed a decrease in the V max without changes in the K m . Results: Our results confirm previous studies suggesting a renal dopaminergic deficiency in insulin-dependent diabetes and provide evidence strongly suggesting that a decrease in the number of tubular L-dopa transport sites is the underlying defect of this deficiency. Conclusion: These results highlight the role of the uptake of dopa as an important modulator of renal dopamine synthesis.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>11805432</pmid><doi>10.1159/000050014</doi><tpages>6</tpages></addata></record> |
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| subjects | 3,4-Dihydroxyphenylacetic Acid - urine Animals Biological Transport Diabetes Mellitus, Experimental - metabolism Dopamine - deficiency Dopamine - metabolism Dose-Response Relationship, Drug Glucose - pharmacology In Vitro Techniques Kidney - metabolism Kidney Tubules, Proximal - metabolism Levodopa - pharmacokinetics Levodopa - pharmacology Levodopa - urine Male Original Paper Osmolar Concentration Rats Rats, Wistar |
| title | Decreased Tubular Uptake of L-3,4-Dihydroxyphenylalanine in Streptozotocin-Induced Diabetic Rats |
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