First report of raspberry yellows disease caused by raspberry bushy dwarf virus in Japan
Severe yellowing of leaves was observed on red raspberry in Akita Prefecture. When inoculated with sap from symptomatic raspberry leaves, Chenopodium quinoa plants developed chlorotic ringspot and mottling that are typical of raspberry bushy dwarf virus (RBDV) infection. In western blot analysis, an...
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| Veröffentlicht in: | Journal of general plant pathology : JGPP 2012-09, Vol.78 (5), p.360-363 |
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| container_title | Journal of general plant pathology : JGPP |
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| creator | Isogai, Masamichi Yoshida, Mari Imanishi, Hiroyuki Yoshikawa, Nobuyuki |
| description | Severe yellowing of leaves was observed on red raspberry in Akita Prefecture. When inoculated with sap from symptomatic raspberry leaves, Chenopodium quinoa plants developed chlorotic ringspot and mottling that are typical of raspberry bushy dwarf virus (RBDV) infection. In western blot analysis, an antibody to the coat protein (CP) of RBDV reacted against ca. 30-kDa protein specific to the diseased trees. In RT-PCR testing for RBDV, single DNA fragments were amplified from total RNA samples of the diseased trees. The nucleotide sequences of the DNA fragments covering the entire CP region revealed 87–97 % identities with those of RBDV isolates. |
| doi_str_mv | 10.1007/s10327-012-0392-8 |
| format | Article |
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When inoculated with sap from symptomatic raspberry leaves, Chenopodium quinoa plants developed chlorotic ringspot and mottling that are typical of raspberry bushy dwarf virus (RBDV) infection. In western blot analysis, an antibody to the coat protein (CP) of RBDV reacted against ca. 30-kDa protein specific to the diseased trees. In RT-PCR testing for RBDV, single DNA fragments were amplified from total RNA samples of the diseased trees. 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The nucleotide sequences of the DNA fragments covering the entire CP region revealed 87–97 % identities with those of RBDV isolates.</description><subject>Agriculture</subject><subject>Berries</subject><subject>Biomedical and Life Sciences</subject><subject>Chenopodium quinoa</subject><subject>Deoxyribonucleic acid</subject><subject>Disease Note</subject><subject>DNA</subject><subject>Fruits</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>nucleotide sequences</subject><subject>Plant diseases</subject><subject>Plant Pathology</subject><subject>Proteins</subject><subject>Raspberry bushy dwarf virus</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>RNA</subject><subject>sap</subject><subject>trees</subject><subject>Western blotting</subject><issn>1345-2630</issn><issn>1610-739X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kD1PwzAQQC0EEqXwA5iwxGzwR1I7I6ooH6rEAJW6WU5yLqlKEnwJVf49rsLQhelueO9OeoRcC34nONf3KLiSmnEhGVeZZOaETMRMcKZVtj6Nu0pSJmeKn5MLxC3nUiidTsh6UQXsaIC2CR1tPA0O2xxCGOgAu12zR1pWCA6BFq5HKGk-HDF5j58DLfcuePpThR5pVdNX17r6kpx5t0O4-ptTslo8fsyf2fLt6WX-sGRFotKO-dQ7U_Kk1MrJHFyhUuGyXHgBOWQeZAbSmFRpcJErEum00QYKrqCIvFZTcjvebUPz3QN2dtv0oY4vrTiUSGdCq0iJkSpCgxjA2zZUXy4MEbKHgHYMaGNAe9CsiY4cHYxsvYFwfPl_6WaUvGus24QK7epdcpFwzk3kjPoFxJB9_A</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Isogai, Masamichi</creator><creator>Yoshida, Mari</creator><creator>Imanishi, Hiroyuki</creator><creator>Yoshikawa, Nobuyuki</creator><general>Springer-Verlag</general><general>Springer Japan</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20120901</creationdate><title>First report of raspberry yellows disease caused by raspberry bushy dwarf virus in Japan</title><author>Isogai, Masamichi ; 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When inoculated with sap from symptomatic raspberry leaves, Chenopodium quinoa plants developed chlorotic ringspot and mottling that are typical of raspberry bushy dwarf virus (RBDV) infection. In western blot analysis, an antibody to the coat protein (CP) of RBDV reacted against ca. 30-kDa protein specific to the diseased trees. In RT-PCR testing for RBDV, single DNA fragments were amplified from total RNA samples of the diseased trees. The nucleotide sequences of the DNA fragments covering the entire CP region revealed 87–97 % identities with those of RBDV isolates.</abstract><cop>Japan</cop><pub>Springer-Verlag</pub><doi>10.1007/s10327-012-0392-8</doi><tpages>4</tpages></addata></record> |
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| subjects | Agriculture Berries Biomedical and Life Sciences Chenopodium quinoa Deoxyribonucleic acid Disease Note DNA Fruits Leaves Life Sciences Microbiology nucleotide sequences Plant diseases Plant Pathology Proteins Raspberry bushy dwarf virus reverse transcriptase polymerase chain reaction RNA sap trees Western blotting |
| title | First report of raspberry yellows disease caused by raspberry bushy dwarf virus in Japan |
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