Generation of Active Bovine Terminal Deoxynucleotidyl Transferase (TdT) in E.coli

Generation of Active Bovine Terminal Deoxynucleotidyl Transferase (TdT) in E.coli

A synthetic gene encoding bovine terminal deoxynucleotidyl transferase (TdT) was generated, cloned into an expression vector and expressed in E.coli. The effects of altering culture and induction conditions on the nature of recombinant protein production were investigated. This led to the expression...

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Veröffentlicht in:Biochemistry Insights 2010-01, Vol.3, p.41
Hauptverfasser: Kuan, Wee Liang, Joy, Joma, Mee, Ng Fui, Perlyn, Kwek Zekui, Wen, Then Siew, Nguen, Thuy, James, Joanne, Chai, Elaine, Flotow, Horst, Crasta, Sharon, Chua, Kelvin, Peng, Ng Sok, Hill, Jeffrey
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container_title Biochemistry Insights
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creator Kuan, Wee Liang
Joy, Joma
Mee, Ng Fui
Perlyn, Kwek Zekui
Wen, Then Siew
Nguen, Thuy
James, Joanne
Chai, Elaine
Flotow, Horst
Crasta, Sharon
Chua, Kelvin
Peng, Ng Sok
Hill, Jeffrey
description A synthetic gene encoding bovine terminal deoxynucleotidyl transferase (TdT) was generated, cloned into an expression vector and expressed in E.coli. The effects of altering culture and induction conditions on the nature of recombinant protein production were investigated. This led to the expression of active recombinant bovine TdT in E.coli. After purification and characterisation, the activity of the enzyme was assessed in a biological assay for apoptosis. The process described in this report enables the economical production of TdT for high throughput applications.
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title Generation of Active Bovine Terminal Deoxynucleotidyl Transferase (TdT) in E.coli
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