Evaluation of the in vitro Inhibitory Impact of Hypericin on Placental Glutathione S-Transferase pi
St John’s Wort (SJW) extracts are herbal products which are available without prescription in most countries and widely used in the treatment of mild to moderate depression. Since it is a herbal product and available without prescription, use of SJW is common among pregnant and/or lactating woman. T...
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| Veröffentlicht in: | The Protein Journal 2012-10, Vol.31 (7), p.544-549 |
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| creator | Dalmizrak, Ozlem Kulaksiz-Erkmen, Gulnihal Ozer, Nazmi |
| description | St John’s Wort (SJW) extracts are herbal products which are available without prescription in most countries and widely used in the treatment of mild to moderate depression. Since it is a herbal product and available without prescription, use of SJW is common among pregnant and/or lactating woman. The principal of the study was to clarify the effects of hypericin, one of the components of SJW, on glutathione S-transferase-pi (GST-pi) purified from human placenta. The
K
m
values of GST-pi were 0.21 ± 0.03 mM for glutathione (GSH) and 2.29 ± 0.54 mM for 1-chloro-2,4-dinitrobenzene (CDNB). At fixed [GSH], the
V
m
value calculated was about 3 times higher than the conditions in which [CDNB] was fixed; 201 ± 30 U/mg protein versus 74 ± 3 U/mg protein. At constant substrate concentrations (1 mM), an average
IC
50
value of 0.70 ± 0.02 μM was obtained. Hypericin inhibited GST-pi competitively with respect to both substrates. When GSH was the varied substrate a
K
i
value of 0.31 ± 0.05 μM was found; when CDNB was the varied substrate, a
K
i
value of 0.85 ± 0.02 μM was obtained. On the basis of these data considering transplacental transfer of hypericin and immature hepatic clearance of the baby, using this herbal product may cause abnormalites due to the inhibition of one of the most important placental detoxification enzymes, GST-pi. |
| doi_str_mv | 10.1007/s10930-012-9433-6 |
| format | Article |
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K
m
values of GST-pi were 0.21 ± 0.03 mM for glutathione (GSH) and 2.29 ± 0.54 mM for 1-chloro-2,4-dinitrobenzene (CDNB). At fixed [GSH], the
V
m
value calculated was about 3 times higher than the conditions in which [CDNB] was fixed; 201 ± 30 U/mg protein versus 74 ± 3 U/mg protein. At constant substrate concentrations (1 mM), an average
IC
50
value of 0.70 ± 0.02 μM was obtained. Hypericin inhibited GST-pi competitively with respect to both substrates. When GSH was the varied substrate a
K
i
value of 0.31 ± 0.05 μM was found; when CDNB was the varied substrate, a
K
i
value of 0.85 ± 0.02 μM was obtained. On the basis of these data considering transplacental transfer of hypericin and immature hepatic clearance of the baby, using this herbal product may cause abnormalites due to the inhibition of one of the most important placental detoxification enzymes, GST-pi.</description><identifier>ISSN: 1572-3887</identifier><identifier>EISSN: 1573-4943</identifier><identifier>EISSN: 1875-8355</identifier><identifier>DOI: 10.1007/s10930-012-9433-6</identifier><identifier>PMID: 22810152</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animal Anatomy ; Biochemistry ; Bioorganic Chemistry ; Birth defects ; Chemistry ; Chemistry and Materials Science ; Detoxification ; Dinitrochlorobenzene ; Electrophoresis, Polyacrylamide Gel ; Enzymes ; Ethylenediaminetetraacetic acid ; Female ; Glutathione - metabolism ; Glutathione S-Transferase pi - antagonists & inhibitors ; Glutathione S-Transferase pi - isolation & purification ; Glutathione S-Transferase pi - metabolism ; Glutathione transferase ; Herbal medicine ; Herbs ; Histology ; Humans ; Hypericum - chemistry ; Kinetics ; Medicine, Botanic ; Medicine, Herbal ; Morphology ; Organic Chemistry ; Perylene - analogs & derivatives ; Perylene - pharmacology ; Placenta - drug effects ; Placenta - enzymology ; Pregnancy ; Proteins ; Substrate Specificity ; Substrates ; Thiols</subject><ispartof>The Protein Journal, 2012-10, Vol.31 (7), p.544-549</ispartof><rights>Springer Science+Business Media, LLC 2012</rights><rights>COPYRIGHT 2012 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-9dc2fdc923a3d0b7fb38626af9d0b1b94c1d772a5d3cf84a7ec461622e3e483d3</citedby><cites>FETCH-LOGICAL-c439t-9dc2fdc923a3d0b7fb38626af9d0b1b94c1d772a5d3cf84a7ec461622e3e483d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10930-012-9433-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10930-012-9433-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22810152$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dalmizrak, Ozlem</creatorcontrib><creatorcontrib>Kulaksiz-Erkmen, Gulnihal</creatorcontrib><creatorcontrib>Ozer, Nazmi</creatorcontrib><title>Evaluation of the in vitro Inhibitory Impact of Hypericin on Placental Glutathione S-Transferase pi</title><title>The Protein Journal</title><addtitle>Protein J</addtitle><addtitle>Protein J</addtitle><description>St John’s Wort (SJW) extracts are herbal products which are available without prescription in most countries and widely used in the treatment of mild to moderate depression. Since it is a herbal product and available without prescription, use of SJW is common among pregnant and/or lactating woman. The principal of the study was to clarify the effects of hypericin, one of the components of SJW, on glutathione S-transferase-pi (GST-pi) purified from human placenta. The
K
m
values of GST-pi were 0.21 ± 0.03 mM for glutathione (GSH) and 2.29 ± 0.54 mM for 1-chloro-2,4-dinitrobenzene (CDNB). At fixed [GSH], the
V
m
value calculated was about 3 times higher than the conditions in which [CDNB] was fixed; 201 ± 30 U/mg protein versus 74 ± 3 U/mg protein. At constant substrate concentrations (1 mM), an average
IC
50
value of 0.70 ± 0.02 μM was obtained. Hypericin inhibited GST-pi competitively with respect to both substrates. When GSH was the varied substrate a
K
i
value of 0.31 ± 0.05 μM was found; when CDNB was the varied substrate, a
K
i
value of 0.85 ± 0.02 μM was obtained. On the basis of these data considering transplacental transfer of hypericin and immature hepatic clearance of the baby, using this herbal product may cause abnormalites due to the inhibition of one of the most important placental detoxification enzymes, GST-pi.</description><subject>Animal Anatomy</subject><subject>Biochemistry</subject><subject>Bioorganic Chemistry</subject><subject>Birth defects</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Detoxification</subject><subject>Dinitrochlorobenzene</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzymes</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Female</subject><subject>Glutathione - metabolism</subject><subject>Glutathione S-Transferase pi - antagonists & inhibitors</subject><subject>Glutathione S-Transferase pi - isolation & purification</subject><subject>Glutathione S-Transferase pi - metabolism</subject><subject>Glutathione transferase</subject><subject>Herbal medicine</subject><subject>Herbs</subject><subject>Histology</subject><subject>Humans</subject><subject>Hypericum - 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metabolism</topic><topic>Glutathione S-Transferase pi - antagonists & inhibitors</topic><topic>Glutathione S-Transferase pi - isolation & purification</topic><topic>Glutathione S-Transferase pi - metabolism</topic><topic>Glutathione transferase</topic><topic>Herbal medicine</topic><topic>Herbs</topic><topic>Histology</topic><topic>Humans</topic><topic>Hypericum - chemistry</topic><topic>Kinetics</topic><topic>Medicine, Botanic</topic><topic>Medicine, Herbal</topic><topic>Morphology</topic><topic>Organic Chemistry</topic><topic>Perylene - analogs & derivatives</topic><topic>Perylene - pharmacology</topic><topic>Placenta - drug effects</topic><topic>Placenta - enzymology</topic><topic>Pregnancy</topic><topic>Proteins</topic><topic>Substrate Specificity</topic><topic>Substrates</topic><topic>Thiols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dalmizrak, Ozlem</creatorcontrib><creatorcontrib>Kulaksiz-Erkmen, Gulnihal</creatorcontrib><creatorcontrib>Ozer, Nazmi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>The Protein Journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dalmizrak, Ozlem</au><au>Kulaksiz-Erkmen, Gulnihal</au><au>Ozer, Nazmi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the in vitro Inhibitory Impact of Hypericin on Placental Glutathione S-Transferase pi</atitle><jtitle>The Protein Journal</jtitle><stitle>Protein J</stitle><addtitle>Protein J</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>31</volume><issue>7</issue><spage>544</spage><epage>549</epage><pages>544-549</pages><issn>1572-3887</issn><eissn>1573-4943</eissn><eissn>1875-8355</eissn><abstract>St John’s Wort (SJW) extracts are herbal products which are available without prescription in most countries and widely used in the treatment of mild to moderate depression. Since it is a herbal product and available without prescription, use of SJW is common among pregnant and/or lactating woman. The principal of the study was to clarify the effects of hypericin, one of the components of SJW, on glutathione S-transferase-pi (GST-pi) purified from human placenta. The
K
m
values of GST-pi were 0.21 ± 0.03 mM for glutathione (GSH) and 2.29 ± 0.54 mM for 1-chloro-2,4-dinitrobenzene (CDNB). At fixed [GSH], the
V
m
value calculated was about 3 times higher than the conditions in which [CDNB] was fixed; 201 ± 30 U/mg protein versus 74 ± 3 U/mg protein. At constant substrate concentrations (1 mM), an average
IC
50
value of 0.70 ± 0.02 μM was obtained. Hypericin inhibited GST-pi competitively with respect to both substrates. When GSH was the varied substrate a
K
i
value of 0.31 ± 0.05 μM was found; when CDNB was the varied substrate, a
K
i
value of 0.85 ± 0.02 μM was obtained. On the basis of these data considering transplacental transfer of hypericin and immature hepatic clearance of the baby, using this herbal product may cause abnormalites due to the inhibition of one of the most important placental detoxification enzymes, GST-pi.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>22810152</pmid><doi>10.1007/s10930-012-9433-6</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1572-3887 |
| ispartof | The Protein Journal, 2012-10, Vol.31 (7), p.544-549 |
| issn | 1572-3887 1573-4943 1875-8355 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animal Anatomy Biochemistry Bioorganic Chemistry Birth defects Chemistry Chemistry and Materials Science Detoxification Dinitrochlorobenzene Electrophoresis, Polyacrylamide Gel Enzymes Ethylenediaminetetraacetic acid Female Glutathione - metabolism Glutathione S-Transferase pi - antagonists & inhibitors Glutathione S-Transferase pi - isolation & purification Glutathione S-Transferase pi - metabolism Glutathione transferase Herbal medicine Herbs Histology Humans Hypericum - chemistry Kinetics Medicine, Botanic Medicine, Herbal Morphology Organic Chemistry Perylene - analogs & derivatives Perylene - pharmacology Placenta - drug effects Placenta - enzymology Pregnancy Proteins Substrate Specificity Substrates Thiols |
| title | Evaluation of the in vitro Inhibitory Impact of Hypericin on Placental Glutathione S-Transferase pi |
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