FRET measurements of intracellular cAMP concentrations and cAMP analog permeability in intact cells

Real-time measurements of second messengers in living cells, such as cAMP, are usually performed by ratiometric fluorescence resonance energy transfer (FRET) imaging. However, correct calibration of FRET ratios, accurate calculations of absolute cAMP levels and actual permeabilities of different cAM...

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Veröffentlicht in:	Nature protocols 2011-04, Vol.6 (4), p.427-438
Hauptverfasser:	Börner, Sebastian, Schwede, Frank, Schlipp, Angela, Berisha, Filip, Calebiro, Davide, Lohse, Martin J, Nikolaev, Viacheslav O
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/527/2047 631/80 Adenylyl Cyclase Inhibitors Analytical Chemistry Animals Biological Techniques Biomedical and Life Sciences Biosensing Techniques Biosensors Calibration Camps Cell Membrane Permeability Cells Cells, Cultured Computational Biology/Bioinformatics Cyclic adenylic acid Cyclic AMP - metabolism Energy transfer Fluorescence Fluorescence Resonance Energy Transfer - methods Guanine Nucleotide Exchange Factors - analysis Guanine Nucleotide Exchange Factors - genetics Guanine Nucleotide Exchange Factors - metabolism Kinetics Life Sciences Measurement Metabolites Mice Mice, Transgenic Microarrays Myocytes, Cardiac - metabolism Organic Chemistry Permeability Physiological aspects Proteins Protocol Resonance Sensors Thyroid Thyroid Gland - metabolism
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