The EB-DNA Liquid Crystalline Complex with High Concentration of Mg2
The effects of ethidium bromide (EB) on the DNA cholesteric phase in the high ionic strength of Mg 2+ were investigated by polarized optical microscopy (POM), 31 P and 23 Na nuclear magnetic resonance (NMR). It was found that the cholesteric pitch in the EB-DNA cholesteric phase is obviously smaller...
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| Veröffentlicht in: | Polymer journal 2006-06, Vol.38 (6), p.597-602 |
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| creator | Liao, Bo He, Benqiao Chen, Shouxi Huang, Yong |
| description | The effects of ethidium bromide (EB) on the DNA cholesteric phase in the high ionic strength of Mg
2+
were investigated by polarized optical microscopy (POM),
31
P and
23
Na nuclear magnetic resonance (NMR). It was found that the cholesteric pitch in the EB-DNA cholesteric phase is obviously smaller than that in the EB-free DNA cholesteric solutions with the same ionic strength of Mg
2+
at room temperature. The fraction of liquid crystal phase in both the EB-free and the EB-DNA solutions is decreased with increasing the Mg
2+
concentration. But the fraction of liquid crystal phase in the EB-DNA solutions is higher than that in the EB-free DNA solutions at the same Mg
2+
concentration, which is attributed to the change of the surface charge distribution on the DNA chains and the stiffness of DNA due to intercalation of EB. The results of the
23
Na NMR experiments suggested that the intercalation of EB results in the changes of the ionic environment close to the DNA chains, by which the liquid crystal behavior of DNA is affected. |
| doi_str_mv | 10.1295/polymj.PJ2005210 |
| format | Article |
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2+
were investigated by polarized optical microscopy (POM),
31
P and
23
Na nuclear magnetic resonance (NMR). It was found that the cholesteric pitch in the EB-DNA cholesteric phase is obviously smaller than that in the EB-free DNA cholesteric solutions with the same ionic strength of Mg
2+
at room temperature. The fraction of liquid crystal phase in both the EB-free and the EB-DNA solutions is decreased with increasing the Mg
2+
concentration. But the fraction of liquid crystal phase in the EB-DNA solutions is higher than that in the EB-free DNA solutions at the same Mg
2+
concentration, which is attributed to the change of the surface charge distribution on the DNA chains and the stiffness of DNA due to intercalation of EB. The results of the
23
Na NMR experiments suggested that the intercalation of EB results in the changes of the ionic environment close to the DNA chains, by which the liquid crystal behavior of DNA is affected.</description><identifier>ISSN: 0032-3896</identifier><identifier>EISSN: 1349-0540</identifier><identifier>DOI: 10.1295/polymj.PJ2005210</identifier><identifier>CODEN: POLJB8</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Analytical, structural and metabolic biochemistry ; Applied sciences ; award-account ; Biological and medical sciences ; Biomaterials ; Bioorganic Chemistry ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Dna, deoxyribonucleoproteins ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Miscellaneous ; Natural polymers ; Nucleic acids ; Physicochemistry of polymers ; Polymer Sciences ; Surfaces and Interfaces ; Thin Films</subject><ispartof>Polymer journal, 2006-06, Vol.38 (6), p.597-602</ispartof><rights>The Society of Polymer Science, Japan 2006</rights><rights>2006 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Jun 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-ec6c7a40f19ac6e79577c0753f910e03e253b793adf24b5df939a6898d962ab53</citedby><cites>FETCH-LOGICAL-c315t-ec6c7a40f19ac6e79577c0753f910e03e253b793adf24b5df939a6898d962ab53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17997187$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Liao, Bo</creatorcontrib><creatorcontrib>He, Benqiao</creatorcontrib><creatorcontrib>Chen, Shouxi</creatorcontrib><creatorcontrib>Huang, Yong</creatorcontrib><title>The EB-DNA Liquid Crystalline Complex with High Concentration of Mg2</title><title>Polymer journal</title><addtitle>Polym J</addtitle><description>The effects of ethidium bromide (EB) on the DNA cholesteric phase in the high ionic strength of Mg
2+
were investigated by polarized optical microscopy (POM),
31
P and
23
Na nuclear magnetic resonance (NMR). It was found that the cholesteric pitch in the EB-DNA cholesteric phase is obviously smaller than that in the EB-free DNA cholesteric solutions with the same ionic strength of Mg
2+
at room temperature. The fraction of liquid crystal phase in both the EB-free and the EB-DNA solutions is decreased with increasing the Mg
2+
concentration. But the fraction of liquid crystal phase in the EB-DNA solutions is higher than that in the EB-free DNA solutions at the same Mg
2+
concentration, which is attributed to the change of the surface charge distribution on the DNA chains and the stiffness of DNA due to intercalation of EB. The results of the
23
Na NMR experiments suggested that the intercalation of EB results in the changes of the ionic environment close to the DNA chains, by which the liquid crystal behavior of DNA is affected.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Applied sciences</subject><subject>award-account</subject><subject>Biological and medical sciences</subject><subject>Biomaterials</subject><subject>Bioorganic Chemistry</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Dna, deoxyribonucleoproteins</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Miscellaneous</subject><subject>Natural polymers</subject><subject>Nucleic acids</subject><subject>Physicochemistry of polymers</subject><subject>Polymer Sciences</subject><subject>Surfaces and Interfaces</subject><subject>Thin Films</subject><issn>0032-3896</issn><issn>1349-0540</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kL1PwzAQxS0EEqWwM1pCjClnO47jsYRCQeVjKHPkOnbrKk1SOxX0vycoFbAwnXT3e--eHkKXBEaESn7T1OV-sx69PVEATgkcoQFhsYyAx3CMBgCMRiyVySk6C2ENQBMO8QDdzVcGT26ju5cxnrntzhU48_vQqrJ0lcFZvWlK84k_XLvCU7dcdZtKm6r1qnV1hWuLn5f0HJ1YVQZzcZhD9H4_mWfTaPb68JiNZ5FmhLeR0YkWKgZLpNKJEZILoUFwZiUBA8xQzhZCMlVYGi94YSWTKkllWsiEqgVnQ3TV-za-3u5MaPN1vfNV9zInwIAwQgXrKOgp7esQvLF5491G-X0H5d9d5X1X-U9XneT6YKyCVqX1qtIu_OqElIKkouNIz4XuVC2N_xvgH-8v5JZ5gg</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Liao, Bo</creator><creator>He, Benqiao</creator><creator>Chen, Shouxi</creator><creator>Huang, Yong</creator><general>Nature Publishing Group UK</general><general>Society of Polymer Science</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20060601</creationdate><title>The EB-DNA Liquid Crystalline Complex with High Concentration of Mg2</title><author>Liao, Bo ; He, Benqiao ; Chen, Shouxi ; Huang, Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-ec6c7a40f19ac6e79577c0753f910e03e253b793adf24b5df939a6898d962ab53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Applied sciences</topic><topic>award-account</topic><topic>Biological and medical sciences</topic><topic>Biomaterials</topic><topic>Bioorganic Chemistry</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Dna, deoxyribonucleoproteins</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Miscellaneous</topic><topic>Natural polymers</topic><topic>Nucleic acids</topic><topic>Physicochemistry of polymers</topic><topic>Polymer Sciences</topic><topic>Surfaces and Interfaces</topic><topic>Thin Films</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liao, Bo</creatorcontrib><creatorcontrib>He, Benqiao</creatorcontrib><creatorcontrib>Chen, Shouxi</creatorcontrib><creatorcontrib>Huang, Yong</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Polymer journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liao, Bo</au><au>He, Benqiao</au><au>Chen, Shouxi</au><au>Huang, Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The EB-DNA Liquid Crystalline Complex with High Concentration of Mg2</atitle><jtitle>Polymer journal</jtitle><stitle>Polym J</stitle><date>2006-06-01</date><risdate>2006</risdate><volume>38</volume><issue>6</issue><spage>597</spage><epage>602</epage><pages>597-602</pages><issn>0032-3896</issn><eissn>1349-0540</eissn><coden>POLJB8</coden><abstract>The effects of ethidium bromide (EB) on the DNA cholesteric phase in the high ionic strength of Mg
2+
were investigated by polarized optical microscopy (POM),
31
P and
23
Na nuclear magnetic resonance (NMR). It was found that the cholesteric pitch in the EB-DNA cholesteric phase is obviously smaller than that in the EB-free DNA cholesteric solutions with the same ionic strength of Mg
2+
at room temperature. The fraction of liquid crystal phase in both the EB-free and the EB-DNA solutions is decreased with increasing the Mg
2+
concentration. But the fraction of liquid crystal phase in the EB-DNA solutions is higher than that in the EB-free DNA solutions at the same Mg
2+
concentration, which is attributed to the change of the surface charge distribution on the DNA chains and the stiffness of DNA due to intercalation of EB. The results of the
23
Na NMR experiments suggested that the intercalation of EB results in the changes of the ionic environment close to the DNA chains, by which the liquid crystal behavior of DNA is affected.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><doi>10.1295/polymj.PJ2005210</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Applied sciences award-account Biological and medical sciences Biomaterials Bioorganic Chemistry Chemistry Chemistry and Materials Science Chemistry/Food Science Dna, deoxyribonucleoproteins Exact sciences and technology Fundamental and applied biological sciences. Psychology Miscellaneous Natural polymers Nucleic acids Physicochemistry of polymers Polymer Sciences Surfaces and Interfaces Thin Films |
| title | The EB-DNA Liquid Crystalline Complex with High Concentration of Mg2 |
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