Responses of Phanerochaete chrysosporium to Toxic Pollutants: Physiological Flux, Oxidative Stress, and Detoxification
The white-rot fungus Phanerochaete chrysosporium has been widely used for the treatment of waste streams containing heavy metals and toxic organic pollutants. The development of fungal-based treatment technologies requires detailed knowledge of the relationship between bulk water quality and the phy...
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| Veröffentlicht in: | Environmental science & technology 2012-07, Vol.46 (14), p.7818-7825 |
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| creator | Zeng, Guang-Ming Chen, An-Wei Chen, Gui-Qiu Hu, Xin-Jiang Guan, Song Shang, Cui Lu, Lun-Hui Zou, Zheng-Jun |
| description | The white-rot fungus Phanerochaete chrysosporium has been widely used for the treatment of waste streams containing heavy metals and toxic organic pollutants. The development of fungal-based treatment technologies requires detailed knowledge of the relationship between bulk water quality and the physiological responses of fungi. A noninvasive microtest technique was used to quantify real-time changes in proton, oxygen, and cadmium ion fluxes following the exposure of P. chrysosporium to environmental toxic (2,4-dichlorophenol and cadmium). Significant changes in H+ and O2 flux occurred after exposure to 10 mg/L 2,4-dichlorophenol and 0.1 mM cadmium. Cd2+ flux decreased with time. Reactive oxygen species formation and antioxidant levels increased after cadmium treatment. Superoxide dismutase activity correlated well with malondialdehyde levels (r2 = 0.964) at low cadmium concentrations. However, this correlation diminished and malondialdehyde levels significantly increased at the highest cadmium concentration tested. Real-time microscale signatures of H+, O2, and Cd2+ fluxes coupled with oxidative stress analysis can improve our understanding of the physiological responses of P. chrysosporium to toxic pollutants and provide useful information for the development of fungal-based technologies to improve the treatment of wastes cocontaminated with heavy metals and organic pollutants. |
| doi_str_mv | 10.1021/es301006j |
| format | Article |
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The development of fungal-based treatment technologies requires detailed knowledge of the relationship between bulk water quality and the physiological responses of fungi. A noninvasive microtest technique was used to quantify real-time changes in proton, oxygen, and cadmium ion fluxes following the exposure of P. chrysosporium to environmental toxic (2,4-dichlorophenol and cadmium). Significant changes in H+ and O2 flux occurred after exposure to 10 mg/L 2,4-dichlorophenol and 0.1 mM cadmium. Cd2+ flux decreased with time. Reactive oxygen species formation and antioxidant levels increased after cadmium treatment. Superoxide dismutase activity correlated well with malondialdehyde levels (r2 = 0.964) at low cadmium concentrations. However, this correlation diminished and malondialdehyde levels significantly increased at the highest cadmium concentration tested. Real-time microscale signatures of H+, O2, and Cd2+ fluxes coupled with oxidative stress analysis can improve our understanding of the physiological responses of P. chrysosporium to toxic pollutants and provide useful information for the development of fungal-based technologies to improve the treatment of wastes cocontaminated with heavy metals and organic pollutants.</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/es301006j</identifier><identifier>PMID: 22703191</identifier><identifier>CODEN: ESTHAG</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Applied sciences ; Biological and medical sciences ; Biological treatment of waters ; Biotechnology ; Cadmium ; Cadmium - toxicity ; Chlorophenols - toxicity ; Environment and pollution ; Environmental Pollutants - toxicity ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Fungi ; General purification processes ; Heavy metals ; Inactivation, Metabolic ; Industrial applications and implications. Economical aspects ; Malondialdehyde - metabolism ; Oxidative stress ; Oxidative Stress - drug effects ; Oxygen - metabolism ; Phanerochaete - drug effects ; Phanerochaete - enzymology ; Phanerochaete - physiology ; Physiology ; Pollutants ; Pollution ; Protons ; Stress analysis ; Stress, Physiological - drug effects ; Superoxide Dismutase - metabolism ; Time Factors ; Toxicity Tests - methods ; Wastewaters ; Water quality ; Water treatment ; Water treatment and pollution</subject><ispartof>Environmental science & technology, 2012-07, Vol.46 (14), p.7818-7825</ispartof><rights>Copyright © 2012 American Chemical Society</rights><rights>2014 INIST-CNRS</rights><rights>Copyright American Chemical Society Jul 17, 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a373t-232d90144d16bca8facddee21360f7501a2660bad6d339299e787c03637104673</citedby><cites>FETCH-LOGICAL-a373t-232d90144d16bca8facddee21360f7501a2660bad6d339299e787c03637104673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/es301006j$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/es301006j$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26163370$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22703191$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeng, Guang-Ming</creatorcontrib><creatorcontrib>Chen, An-Wei</creatorcontrib><creatorcontrib>Chen, Gui-Qiu</creatorcontrib><creatorcontrib>Hu, Xin-Jiang</creatorcontrib><creatorcontrib>Guan, Song</creatorcontrib><creatorcontrib>Shang, Cui</creatorcontrib><creatorcontrib>Lu, Lun-Hui</creatorcontrib><creatorcontrib>Zou, Zheng-Jun</creatorcontrib><title>Responses of Phanerochaete chrysosporium to Toxic Pollutants: Physiological Flux, Oxidative Stress, and Detoxification</title><title>Environmental science & technology</title><addtitle>Environ. Sci. Technol</addtitle><description>The white-rot fungus Phanerochaete chrysosporium has been widely used for the treatment of waste streams containing heavy metals and toxic organic pollutants. The development of fungal-based treatment technologies requires detailed knowledge of the relationship between bulk water quality and the physiological responses of fungi. A noninvasive microtest technique was used to quantify real-time changes in proton, oxygen, and cadmium ion fluxes following the exposure of P. chrysosporium to environmental toxic (2,4-dichlorophenol and cadmium). Significant changes in H+ and O2 flux occurred after exposure to 10 mg/L 2,4-dichlorophenol and 0.1 mM cadmium. Cd2+ flux decreased with time. Reactive oxygen species formation and antioxidant levels increased after cadmium treatment. Superoxide dismutase activity correlated well with malondialdehyde levels (r2 = 0.964) at low cadmium concentrations. However, this correlation diminished and malondialdehyde levels significantly increased at the highest cadmium concentration tested. Real-time microscale signatures of H+, O2, and Cd2+ fluxes coupled with oxidative stress analysis can improve our understanding of the physiological responses of P. chrysosporium to toxic pollutants and provide useful information for the development of fungal-based technologies to improve the treatment of wastes cocontaminated with heavy metals and organic pollutants.</description><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biological treatment of waters</subject><subject>Biotechnology</subject><subject>Cadmium</subject><subject>Cadmium - toxicity</subject><subject>Chlorophenols - toxicity</subject><subject>Environment and pollution</subject><subject>Environmental Pollutants - toxicity</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>General purification processes</subject><subject>Heavy metals</subject><subject>Inactivation, Metabolic</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Malondialdehyde - metabolism</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Oxygen - metabolism</subject><subject>Phanerochaete - drug effects</subject><subject>Phanerochaete - enzymology</subject><subject>Phanerochaete - physiology</subject><subject>Physiology</subject><subject>Pollutants</subject><subject>Pollution</subject><subject>Protons</subject><subject>Stress analysis</subject><subject>Stress, Physiological - drug effects</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Time Factors</subject><subject>Toxicity Tests - methods</subject><subject>Wastewaters</subject><subject>Water quality</subject><subject>Water treatment</subject><subject>Water treatment and pollution</subject><issn>0013-936X</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0EtLAzEQB_AgitbHwS8gAfEgdHWSdLNdb-IbBMUHeCtpkrUp203NZKX99kZaq3iaw_zmwZ-QfQYnDDg7tSiAAcjxGumwnEOW93O2TjoATGSlkG9bZBtxDABcQH-TbHFegGAl65DPJ4tT36BF6iv6OFKNDV6PlI2W6lGYo0_t4NoJjZ6--JnT9NHXdRtVE_EsDczR-dq_O61qel23sy59mDmjovu09DkGi9ilqjH00sY0XSUXnW92yUalarR7y7pDXq-vXi5us_uHm7uL8_tMiULEjAtuSmC9nmFyqFW_UtoYazkTEqoiB6a4lDBURhohSl6WtugXGoQUBYOeLMQOOVzsnQb_0VqMg7FvQ5NODlJyBZSlzL_V8ULp4BGDrQbT4CYqzBP6dmywSjjZg-XGdjixZiV_Ik3gaAkUplCqoBrt8NdJJoVIduWUxr9f_T_4BQoMj6E</recordid><startdate>20120717</startdate><enddate>20120717</enddate><creator>Zeng, Guang-Ming</creator><creator>Chen, An-Wei</creator><creator>Chen, Gui-Qiu</creator><creator>Hu, Xin-Jiang</creator><creator>Guan, Song</creator><creator>Shang, Cui</creator><creator>Lu, Lun-Hui</creator><creator>Zou, Zheng-Jun</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>20120717</creationdate><title>Responses of Phanerochaete chrysosporium to Toxic Pollutants: Physiological Flux, Oxidative Stress, and Detoxification</title><author>Zeng, Guang-Ming ; Chen, An-Wei ; Chen, Gui-Qiu ; Hu, Xin-Jiang ; Guan, Song ; Shang, Cui ; Lu, Lun-Hui ; Zou, Zheng-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a373t-232d90144d16bca8facddee21360f7501a2660bad6d339299e787c03637104673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Biological treatment of waters</topic><topic>Biotechnology</topic><topic>Cadmium</topic><topic>Cadmium - toxicity</topic><topic>Chlorophenols - toxicity</topic><topic>Environment and pollution</topic><topic>Environmental Pollutants - toxicity</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi</topic><topic>General purification processes</topic><topic>Heavy metals</topic><topic>Inactivation, Metabolic</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Malondialdehyde - metabolism</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Oxygen - metabolism</topic><topic>Phanerochaete - drug effects</topic><topic>Phanerochaete - enzymology</topic><topic>Phanerochaete - physiology</topic><topic>Physiology</topic><topic>Pollutants</topic><topic>Pollution</topic><topic>Protons</topic><topic>Stress analysis</topic><topic>Stress, Physiological - drug effects</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Time Factors</topic><topic>Toxicity Tests - methods</topic><topic>Wastewaters</topic><topic>Water quality</topic><topic>Water treatment</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeng, Guang-Ming</creatorcontrib><creatorcontrib>Chen, An-Wei</creatorcontrib><creatorcontrib>Chen, Gui-Qiu</creatorcontrib><creatorcontrib>Hu, Xin-Jiang</creatorcontrib><creatorcontrib>Guan, Song</creatorcontrib><creatorcontrib>Shang, Cui</creatorcontrib><creatorcontrib>Lu, Lun-Hui</creatorcontrib><creatorcontrib>Zou, Zheng-Jun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Environmental science & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Guang-Ming</au><au>Chen, An-Wei</au><au>Chen, Gui-Qiu</au><au>Hu, Xin-Jiang</au><au>Guan, Song</au><au>Shang, Cui</au><au>Lu, Lun-Hui</au><au>Zou, Zheng-Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Responses of Phanerochaete chrysosporium to Toxic Pollutants: Physiological Flux, Oxidative Stress, and Detoxification</atitle><jtitle>Environmental science & technology</jtitle><addtitle>Environ. Sci. Technol</addtitle><date>2012-07-17</date><risdate>2012</risdate><volume>46</volume><issue>14</issue><spage>7818</spage><epage>7825</epage><pages>7818-7825</pages><issn>0013-936X</issn><eissn>1520-5851</eissn><coden>ESTHAG</coden><abstract>The white-rot fungus Phanerochaete chrysosporium has been widely used for the treatment of waste streams containing heavy metals and toxic organic pollutants. The development of fungal-based treatment technologies requires detailed knowledge of the relationship between bulk water quality and the physiological responses of fungi. A noninvasive microtest technique was used to quantify real-time changes in proton, oxygen, and cadmium ion fluxes following the exposure of P. chrysosporium to environmental toxic (2,4-dichlorophenol and cadmium). Significant changes in H+ and O2 flux occurred after exposure to 10 mg/L 2,4-dichlorophenol and 0.1 mM cadmium. Cd2+ flux decreased with time. Reactive oxygen species formation and antioxidant levels increased after cadmium treatment. Superoxide dismutase activity correlated well with malondialdehyde levels (r2 = 0.964) at low cadmium concentrations. However, this correlation diminished and malondialdehyde levels significantly increased at the highest cadmium concentration tested. Real-time microscale signatures of H+, O2, and Cd2+ fluxes coupled with oxidative stress analysis can improve our understanding of the physiological responses of P. chrysosporium to toxic pollutants and provide useful information for the development of fungal-based technologies to improve the treatment of wastes cocontaminated with heavy metals and organic pollutants.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>22703191</pmid><doi>10.1021/es301006j</doi><tpages>8</tpages></addata></record> |
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| subjects | Applied sciences Biological and medical sciences Biological treatment of waters Biotechnology Cadmium Cadmium - toxicity Chlorophenols - toxicity Environment and pollution Environmental Pollutants - toxicity Exact sciences and technology Fundamental and applied biological sciences. Psychology Fungi General purification processes Heavy metals Inactivation, Metabolic Industrial applications and implications. Economical aspects Malondialdehyde - metabolism Oxidative stress Oxidative Stress - drug effects Oxygen - metabolism Phanerochaete - drug effects Phanerochaete - enzymology Phanerochaete - physiology Physiology Pollutants Pollution Protons Stress analysis Stress, Physiological - drug effects Superoxide Dismutase - metabolism Time Factors Toxicity Tests - methods Wastewaters Water quality Water treatment Water treatment and pollution |
| title | Responses of Phanerochaete chrysosporium to Toxic Pollutants: Physiological Flux, Oxidative Stress, and Detoxification |
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