High-Throughput Method Development for Sensitive, Accurate, and Reproducible Quantification of Therapeutic Monoclonal Antibodies in Tissues Using Orthogonal Array Optimization and Nano Liquid Chromatography/Selected Reaction Monitoring Mass Spectrometry

Although liquid chromatography/mass spectrometry using selected reaction monitoring (LC/SRM-MS) holds great promise for targeted protein analysis, quantification of therapeutic monoclonal antibody (mAb) in tissues represents a daunting challenge due to the extremely low tissue levels, complexity of...

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Veröffentlicht in:	Analytical chemistry (Washington) 2012-05, Vol.84 (10), p.4373-4382
Hauptverfasser:	Duan, Xiaotao, Abuqayyas, Lubna, Dai, Lipeng, Balthasar, Joseph P, Qu, Jun
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical chemistry Animals Antibodies, Monoclonal - analysis Antibodies, Monoclonal - metabolism Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography Chromatography, High Pressure Liquid Drug Evaluation, Preclinical Exact sciences and technology High-Throughput Screening Assays liquid chromatography Mass Spectrometry Mice monitoring Monoclonal antibodies Nanotechnology Other chromatographic methods peptides Peptides - analysis Proteins quantitative analysis Spectrometric and optical methods statistical analysis Tissue Distribution Tissues
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