Impaired cell functions of hepatocytes incubated with plasma of septic patients
Objective and design The development of liver failure is a major problem in septic patients. In this prospective clinical experimental study the hepatotoxicity of plasma from septic and non-septic patients was tested. Methods and subjects The basic test components consist of human liver cells (HepG2...
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| Veröffentlicht in: | Inflammation research 2012-06, Vol.61 (6), p.609-616 |
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| creator | Sauer, Martin Haubner, Cristof Mencke, Thomas Nöldge-Schomburg, Gabriele Mitzner, Steffen Altrichter, Jens Stange, Jan |
| description | Objective and design
The development of liver failure is a major problem in septic patients. In this prospective clinical experimental study the hepatotoxicity of plasma from septic and non-septic patients was tested.
Methods and subjects
The basic test components consist of human liver cells (HepG2/C3A) used in a standardized microtiter plate assay. After incubation with patient’s plasma viability of cells (XTT-test), the cytochrome 1A2 activity and synthesis of micro albumin were measured. Subjects (28) enrolled comprise the septic shock group (SSG,
n
= 10), the non-septic group (NSG,
n
= 5) and the healthy volunteers group (HVG,
n
= 13).
Results
The 28-day mortality was 30% in the SSG. The APACHE II-, SOFA-, and SAPS-scores and the values of bilirubin and prothrombin time as INR were significantly higher in the SSG than in the NSG. The cytochrome 1A2 activity and the release of albumin were significantly reduced in HepG2/C3A cells incubated with plasma of the SSG (
p
|
| doi_str_mv | 10.1007/s00011-012-0451-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1013467143</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2660411981</sourcerecordid><originalsourceid>FETCH-LOGICAL-c339t-e80777fc6d7c56e38f171e13ffe26371a929f811b2bb87f496282ca57baeb6363</originalsourceid><addsrcrecordid>eNp1kE1LAzEQhoMotlZ_gBdZ8BzNJNtNcpTiR6HQi4K3kE0Tu2W_TLJI_70pW8WLpxmYZ94ZHoSugdwBIfw-EEIAMAGKST4HLE_QFHJKsCTi_TT1hDLMBCMTdBHCLtGCCnqOJpQyTiQnU7ReNr2uvN1kxtZ15obWxKprQ9a5bGt7HTuzjzZkVWuGUsfEfVVxm_W1Do0-QMH2sTJZIivbxnCJzpyug7061hl6e3p8Xbzg1fp5uXhYYcOYjNgKwjl3pthwMy8sEw44WGDOWVowDlpS6QRASctScJfLIj1u9JyX2pYFK9gM3Y65ve8-Bxui2nWDb9NJBQRYXnDIWaJgpIzvQvDWqd5Xjfb7BKmDQjUqVEmhOihUMu3cHJOHsrGb340fZwmgIxDSqP2w_u_p_1K_Ackpe6A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1013467143</pqid></control><display><type>article</type><title>Impaired cell functions of hepatocytes incubated with plasma of septic patients</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Sauer, Martin ; Haubner, Cristof ; Mencke, Thomas ; Nöldge-Schomburg, Gabriele ; Mitzner, Steffen ; Altrichter, Jens ; Stange, Jan</creator><creatorcontrib>Sauer, Martin ; Haubner, Cristof ; Mencke, Thomas ; Nöldge-Schomburg, Gabriele ; Mitzner, Steffen ; Altrichter, Jens ; Stange, Jan</creatorcontrib><description>Objective and design
The development of liver failure is a major problem in septic patients. In this prospective clinical experimental study the hepatotoxicity of plasma from septic and non-septic patients was tested.
Methods and subjects
The basic test components consist of human liver cells (HepG2/C3A) used in a standardized microtiter plate assay. After incubation with patient’s plasma viability of cells (XTT-test), the cytochrome 1A2 activity and synthesis of micro albumin were measured. Subjects (28) enrolled comprise the septic shock group (SSG,
n
= 10), the non-septic group (NSG,
n
= 5) and the healthy volunteers group (HVG,
n
= 13).
Results
The 28-day mortality was 30% in the SSG. The APACHE II-, SOFA-, and SAPS-scores and the values of bilirubin and prothrombin time as INR were significantly higher in the SSG than in the NSG. The cytochrome 1A2 activity and the release of albumin were significantly reduced in HepG2/C3A cells incubated with plasma of the SSG (
p
< 0.05). The cytochrome 1A2 activities were higher in survivors compared to non-survivors at the time point 0 and were increasing in survivors and decreasing in non-survivors within 54 h in the SSG. In the SSG there was a significant decrease in IL-10 and IL-8 between inclusion and 54 h. Values of IL-6, TNF alpha and IL-10 were significantly lower in the NSG compared with the values of the SSG at inclusion and after 54 h.
Conclusion
The plasma of patients with septic shock impaired cellular functions of HepG2/C3A cells.</description><identifier>ISSN: 1023-3830</identifier><identifier>EISSN: 1420-908X</identifier><identifier>DOI: 10.1007/s00011-012-0451-9</identifier><identifier>PMID: 22370970</identifier><language>eng</language><publisher>Basel: SP Birkhäuser Verlag Basel</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Albumins - metabolism ; Allergology ; Bilirubin - blood ; Biomedical and Life Sciences ; Biomedicine ; Case-Control Studies ; Cell Line, Tumor ; Cytochrome P-450 CYP1A2 - metabolism ; Cytokines - blood ; Dermatology ; Female ; Hepatocytes - metabolism ; Humans ; Immunology ; Liver Failure - blood ; Liver Failure - metabolism ; Male ; Middle Aged ; Neurology ; Original Research Paper ; Pharmacology/Toxicology ; Rheumatology ; Sepsis - blood ; Sepsis - metabolism</subject><ispartof>Inflammation research, 2012-06, Vol.61 (6), p.609-616</ispartof><rights>Springer Basel AG 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-e80777fc6d7c56e38f171e13ffe26371a929f811b2bb87f496282ca57baeb6363</citedby><cites>FETCH-LOGICAL-c339t-e80777fc6d7c56e38f171e13ffe26371a929f811b2bb87f496282ca57baeb6363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00011-012-0451-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00011-012-0451-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22370970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sauer, Martin</creatorcontrib><creatorcontrib>Haubner, Cristof</creatorcontrib><creatorcontrib>Mencke, Thomas</creatorcontrib><creatorcontrib>Nöldge-Schomburg, Gabriele</creatorcontrib><creatorcontrib>Mitzner, Steffen</creatorcontrib><creatorcontrib>Altrichter, Jens</creatorcontrib><creatorcontrib>Stange, Jan</creatorcontrib><title>Impaired cell functions of hepatocytes incubated with plasma of septic patients</title><title>Inflammation research</title><addtitle>Inflamm. Res</addtitle><addtitle>Inflamm Res</addtitle><description>Objective and design
The development of liver failure is a major problem in septic patients. In this prospective clinical experimental study the hepatotoxicity of plasma from septic and non-septic patients was tested.
Methods and subjects
The basic test components consist of human liver cells (HepG2/C3A) used in a standardized microtiter plate assay. After incubation with patient’s plasma viability of cells (XTT-test), the cytochrome 1A2 activity and synthesis of micro albumin were measured. Subjects (28) enrolled comprise the septic shock group (SSG,
n
= 10), the non-septic group (NSG,
n
= 5) and the healthy volunteers group (HVG,
n
= 13).
Results
The 28-day mortality was 30% in the SSG. The APACHE II-, SOFA-, and SAPS-scores and the values of bilirubin and prothrombin time as INR were significantly higher in the SSG than in the NSG. The cytochrome 1A2 activity and the release of albumin were significantly reduced in HepG2/C3A cells incubated with plasma of the SSG (
p
< 0.05). The cytochrome 1A2 activities were higher in survivors compared to non-survivors at the time point 0 and were increasing in survivors and decreasing in non-survivors within 54 h in the SSG. In the SSG there was a significant decrease in IL-10 and IL-8 between inclusion and 54 h. Values of IL-6, TNF alpha and IL-10 were significantly lower in the NSG compared with the values of the SSG at inclusion and after 54 h.
Conclusion
The plasma of patients with septic shock impaired cellular functions of HepG2/C3A cells.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Albumins - metabolism</subject><subject>Allergology</subject><subject>Bilirubin - blood</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Case-Control Studies</subject><subject>Cell Line, Tumor</subject><subject>Cytochrome P-450 CYP1A2 - metabolism</subject><subject>Cytokines - blood</subject><subject>Dermatology</subject><subject>Female</subject><subject>Hepatocytes - metabolism</subject><subject>Humans</subject><subject>Immunology</subject><subject>Liver Failure - blood</subject><subject>Liver Failure - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neurology</subject><subject>Original Research Paper</subject><subject>Pharmacology/Toxicology</subject><subject>Rheumatology</subject><subject>Sepsis - blood</subject><subject>Sepsis - metabolism</subject><issn>1023-3830</issn><issn>1420-908X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kE1LAzEQhoMotlZ_gBdZ8BzNJNtNcpTiR6HQi4K3kE0Tu2W_TLJI_70pW8WLpxmYZ94ZHoSugdwBIfw-EEIAMAGKST4HLE_QFHJKsCTi_TT1hDLMBCMTdBHCLtGCCnqOJpQyTiQnU7ReNr2uvN1kxtZ15obWxKprQ9a5bGt7HTuzjzZkVWuGUsfEfVVxm_W1Do0-QMH2sTJZIivbxnCJzpyug7061hl6e3p8Xbzg1fp5uXhYYcOYjNgKwjl3pthwMy8sEw44WGDOWVowDlpS6QRASctScJfLIj1u9JyX2pYFK9gM3Y65ve8-Bxui2nWDb9NJBQRYXnDIWaJgpIzvQvDWqd5Xjfb7BKmDQjUqVEmhOihUMu3cHJOHsrGb340fZwmgIxDSqP2w_u_p_1K_Ackpe6A</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Sauer, Martin</creator><creator>Haubner, Cristof</creator><creator>Mencke, Thomas</creator><creator>Nöldge-Schomburg, Gabriele</creator><creator>Mitzner, Steffen</creator><creator>Altrichter, Jens</creator><creator>Stange, Jan</creator><general>SP Birkhäuser Verlag Basel</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20120601</creationdate><title>Impaired cell functions of hepatocytes incubated with plasma of septic patients</title><author>Sauer, Martin ; Haubner, Cristof ; Mencke, Thomas ; Nöldge-Schomburg, Gabriele ; Mitzner, Steffen ; Altrichter, Jens ; Stange, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-e80777fc6d7c56e38f171e13ffe26371a929f811b2bb87f496282ca57baeb6363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Albumins - metabolism</topic><topic>Allergology</topic><topic>Bilirubin - blood</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Case-Control Studies</topic><topic>Cell Line, Tumor</topic><topic>Cytochrome P-450 CYP1A2 - metabolism</topic><topic>Cytokines - blood</topic><topic>Dermatology</topic><topic>Female</topic><topic>Hepatocytes - metabolism</topic><topic>Humans</topic><topic>Immunology</topic><topic>Liver Failure - blood</topic><topic>Liver Failure - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neurology</topic><topic>Original Research Paper</topic><topic>Pharmacology/Toxicology</topic><topic>Rheumatology</topic><topic>Sepsis - blood</topic><topic>Sepsis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sauer, Martin</creatorcontrib><creatorcontrib>Haubner, Cristof</creatorcontrib><creatorcontrib>Mencke, Thomas</creatorcontrib><creatorcontrib>Nöldge-Schomburg, Gabriele</creatorcontrib><creatorcontrib>Mitzner, Steffen</creatorcontrib><creatorcontrib>Altrichter, Jens</creatorcontrib><creatorcontrib>Stange, Jan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Inflammation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sauer, Martin</au><au>Haubner, Cristof</au><au>Mencke, Thomas</au><au>Nöldge-Schomburg, Gabriele</au><au>Mitzner, Steffen</au><au>Altrichter, Jens</au><au>Stange, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impaired cell functions of hepatocytes incubated with plasma of septic patients</atitle><jtitle>Inflammation research</jtitle><stitle>Inflamm. Res</stitle><addtitle>Inflamm Res</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>61</volume><issue>6</issue><spage>609</spage><epage>616</epage><pages>609-616</pages><issn>1023-3830</issn><eissn>1420-908X</eissn><abstract>Objective and design
The development of liver failure is a major problem in septic patients. In this prospective clinical experimental study the hepatotoxicity of plasma from septic and non-septic patients was tested.
Methods and subjects
The basic test components consist of human liver cells (HepG2/C3A) used in a standardized microtiter plate assay. After incubation with patient’s plasma viability of cells (XTT-test), the cytochrome 1A2 activity and synthesis of micro albumin were measured. Subjects (28) enrolled comprise the septic shock group (SSG,
n
= 10), the non-septic group (NSG,
n
= 5) and the healthy volunteers group (HVG,
n
= 13).
Results
The 28-day mortality was 30% in the SSG. The APACHE II-, SOFA-, and SAPS-scores and the values of bilirubin and prothrombin time as INR were significantly higher in the SSG than in the NSG. The cytochrome 1A2 activity and the release of albumin were significantly reduced in HepG2/C3A cells incubated with plasma of the SSG (
p
< 0.05). The cytochrome 1A2 activities were higher in survivors compared to non-survivors at the time point 0 and were increasing in survivors and decreasing in non-survivors within 54 h in the SSG. In the SSG there was a significant decrease in IL-10 and IL-8 between inclusion and 54 h. Values of IL-6, TNF alpha and IL-10 were significantly lower in the NSG compared with the values of the SSG at inclusion and after 54 h.
Conclusion
The plasma of patients with septic shock impaired cellular functions of HepG2/C3A cells.</abstract><cop>Basel</cop><pub>SP Birkhäuser Verlag Basel</pub><pmid>22370970</pmid><doi>10.1007/s00011-012-0451-9</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1023-3830 |
| ispartof | Inflammation research, 2012-06, Vol.61 (6), p.609-616 |
| issn | 1023-3830 1420-908X |
| language | eng |
| recordid | cdi_proquest_journals_1013467143 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adult Aged Aged, 80 and over Albumins - metabolism Allergology Bilirubin - blood Biomedical and Life Sciences Biomedicine Case-Control Studies Cell Line, Tumor Cytochrome P-450 CYP1A2 - metabolism Cytokines - blood Dermatology Female Hepatocytes - metabolism Humans Immunology Liver Failure - blood Liver Failure - metabolism Male Middle Aged Neurology Original Research Paper Pharmacology/Toxicology Rheumatology Sepsis - blood Sepsis - metabolism |
| title | Impaired cell functions of hepatocytes incubated with plasma of septic patients |
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