Asparagus racemosus cell cultures: a source for enhanced production of shatavarins and sarsapogenin
Asparagus racemosus is an important monocot medicinal plant that is in great demand for its steroidal saponins called shatavarins. This study was initiated to optimize the conditions for production of shatavarins in cell cultures of A. racemosus in a modified Murashige and Skoog (MS) medium suppleme...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2012-02, Vol.48 (1), p.85-91 |
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| container_title | In vitro cellular & developmental biology. Plant |
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| creator | Pise, Mashitha Rudra, Jaishree Bundale, Sunita Begde, Deovrat Nashikkar, Nandita Upadhyay, Avinash |
| description | Asparagus racemosus is an important monocot medicinal plant that is in great demand for its steroidal saponins called shatavarins. This study was initiated to optimize the conditions for production of shatavarins in cell cultures of A. racemosus in a modified Murashige and Skoog (MS) medium supplemented with six different combinations of growth regulators. Biomass accumulation was correlated with saponin production over a 30-d culture cycle. Biomass and saponin accumulation patterns were dependent on combinations of growth regulators and the pH of the medium. Maximum levels of saponin and biomass accumulation were recorded on day 25 of the culture cycle within a pH range of 3.4 to 5.6. Total saponin produced by the in vitro cultures was 20-fold higher than amounts produced by cultivated plants. Saponin accumulation was not a biomass-associated phenomenon; cultures which showed the highest biomass accumulation were not the highest saponin accumulators. Maximum biomass (28.30 ± 0.29 g l−1) and maximum levels of shatavarin IV(11.48 ± 0.61 mg g−1) accumulation was found using a medium containing 2.0 mg l−1 2,4-D, 2 g l−1 casein hydrolysate and 0.005% pectinase. The highest levels of sarsapogenin, secreted and intracellular (4.02 ± 0.09 mg g−1), accumulated using a medium containing 1.0 mg l−1 NAA, 1.0 mg l−1 2,4-D, 0.5 mg l−1 BAP, 2 g l−1 casein hydrolysate and 0.005% pectinase, after 25 d. Shatavarins were secreted into the medium and can be isolated easily for further purification. |
| doi_str_mv | 10.1007/s11627-011-9391-2 |
| format | Article |
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This study was initiated to optimize the conditions for production of shatavarins in cell cultures of A. racemosus in a modified Murashige and Skoog (MS) medium supplemented with six different combinations of growth regulators. Biomass accumulation was correlated with saponin production over a 30-d culture cycle. Biomass and saponin accumulation patterns were dependent on combinations of growth regulators and the pH of the medium. Maximum levels of saponin and biomass accumulation were recorded on day 25 of the culture cycle within a pH range of 3.4 to 5.6. Total saponin produced by the in vitro cultures was 20-fold higher than amounts produced by cultivated plants. Saponin accumulation was not a biomass-associated phenomenon; cultures which showed the highest biomass accumulation were not the highest saponin accumulators. Maximum biomass (28.30 ± 0.29 g l−1) and maximum levels of shatavarin IV(11.48 ± 0.61 mg g−1) accumulation was found using a medium containing 2.0 mg l−1 2,4-D, 2 g l−1 casein hydrolysate and 0.005% pectinase. The highest levels of sarsapogenin, secreted and intracellular (4.02 ± 0.09 mg g−1), accumulated using a medium containing 1.0 mg l−1 NAA, 1.0 mg l−1 2,4-D, 0.5 mg l−1 BAP, 2 g l−1 casein hydrolysate and 0.005% pectinase, after 25 d. Shatavarins were secreted into the medium and can be isolated easily for further purification.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/s11627-011-9391-2</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>2,4-D ; Accumulation ; Asparagus ; Asparagus racemosus ; Biomass ; Biomass production ; Biomedical and Life Sciences ; Callus ; casein hydrolysates ; Cell Biology ; Cell culture ; Cell culture techniques ; Cultivated plants ; Cultured cells ; Developmental Biology ; Growth regulators ; Indian culture ; Life Sciences ; Medicinal plants ; MOLECULAR FARMING/METABOLIC ENGINEERING/SECONDARY METABOLISM ; naphthaleneacetic acid ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Plant growth regulators ; Plant Physiology ; Plant Sciences ; Plants ; polygalacturonase ; Saponins ; steroid saponins ; Studies</subject><ispartof>In vitro cellular & developmental biology. Plant, 2012-02, Vol.48 (1), p.85-91</ispartof><rights>2012 Society for In Vitro Biology</rights><rights>The Society for In Vitro Biology 2011</rights><rights>Copyright Society for In Vitro Biology Feb 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-e3cf356bfe5e1e909df93660e98ec8c56bc8b56590985499dd2967b9066b0f523</citedby><cites>FETCH-LOGICAL-c362t-e3cf356bfe5e1e909df93660e98ec8c56bc8b56590985499dd2967b9066b0f523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/41496442$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/41496442$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27923,27924,41487,42556,51318,58016,58249</link.rule.ids></links><search><creatorcontrib>Pise, Mashitha</creatorcontrib><creatorcontrib>Rudra, Jaishree</creatorcontrib><creatorcontrib>Bundale, Sunita</creatorcontrib><creatorcontrib>Begde, Deovrat</creatorcontrib><creatorcontrib>Nashikkar, Nandita</creatorcontrib><creatorcontrib>Upadhyay, Avinash</creatorcontrib><title>Asparagus racemosus cell cultures: a source for enhanced production of shatavarins and sarsapogenin</title><title>In vitro cellular & developmental biology. Plant</title><addtitle>In Vitro Cell.Dev.Biol.-Plant</addtitle><description>Asparagus racemosus is an important monocot medicinal plant that is in great demand for its steroidal saponins called shatavarins. This study was initiated to optimize the conditions for production of shatavarins in cell cultures of A. racemosus in a modified Murashige and Skoog (MS) medium supplemented with six different combinations of growth regulators. Biomass accumulation was correlated with saponin production over a 30-d culture cycle. Biomass and saponin accumulation patterns were dependent on combinations of growth regulators and the pH of the medium. Maximum levels of saponin and biomass accumulation were recorded on day 25 of the culture cycle within a pH range of 3.4 to 5.6. Total saponin produced by the in vitro cultures was 20-fold higher than amounts produced by cultivated plants. Saponin accumulation was not a biomass-associated phenomenon; cultures which showed the highest biomass accumulation were not the highest saponin accumulators. Maximum biomass (28.30 ± 0.29 g l−1) and maximum levels of shatavarin IV(11.48 ± 0.61 mg g−1) accumulation was found using a medium containing 2.0 mg l−1 2,4-D, 2 g l−1 casein hydrolysate and 0.005% pectinase. The highest levels of sarsapogenin, secreted and intracellular (4.02 ± 0.09 mg g−1), accumulated using a medium containing 1.0 mg l−1 NAA, 1.0 mg l−1 2,4-D, 0.5 mg l−1 BAP, 2 g l−1 casein hydrolysate and 0.005% pectinase, after 25 d. Shatavarins were secreted into the medium and can be isolated easily for further purification.</description><subject>2,4-D</subject><subject>Accumulation</subject><subject>Asparagus</subject><subject>Asparagus racemosus</subject><subject>Biomass</subject><subject>Biomass production</subject><subject>Biomedical and Life Sciences</subject><subject>Callus</subject><subject>casein hydrolysates</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell culture techniques</subject><subject>Cultivated plants</subject><subject>Cultured cells</subject><subject>Developmental Biology</subject><subject>Growth regulators</subject><subject>Indian culture</subject><subject>Life Sciences</subject><subject>Medicinal plants</subject><subject>MOLECULAR FARMING/METABOLIC ENGINEERING/SECONDARY METABOLISM</subject><subject>naphthaleneacetic acid</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant growth regulators</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Plants</subject><subject>polygalacturonase</subject><subject>Saponins</subject><subject>steroid saponins</subject><subject>Studies</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU9LAzEQxRdRsFY_gAcx4Hk1ySbZjbdS_AeCB_Uc0uyk3dImNbMr-O1NWREvnjLwfu_N8FIU54xeM0rrG2RM8bqkjJW60qzkB8WEiVqWXDX6MM9UilKKWh0XJ4hrSimjrJ4UboY7m-xyQJKsg23EPDnYbIgbNv2QAG-JJRiH5ID4mAiElQ0OWrJLsR1c38VAoie4sr39tKkLSGxoCdqEdheXELpwWhx5u0E4-3mnxfv93dv8sXx-eXiaz55LVynel1A5X0m18CCBgaa69bpSioJuwDUuK65ZSCWz0kihddtyreqFpkotqJe8mhZXY24-7WMA7M063x3ySpM70rzJvjpTbKRciogJvNmlbmvTV4b2XG3GLk3u0uy7NPtkPnows2EJ6W_y_6aL0bTGPqbfLYIJrYTY65ej7m00dpk6NO-vnDKR_yYDQlXfXXaKjA</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Pise, Mashitha</creator><creator>Rudra, Jaishree</creator><creator>Bundale, Sunita</creator><creator>Begde, Deovrat</creator><creator>Nashikkar, Nandita</creator><creator>Upadhyay, Avinash</creator><general>Springer-Verlag</general><general>Springer Science + Business Media</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope></search><sort><creationdate>20120201</creationdate><title>Asparagus racemosus cell cultures: a source for enhanced production of shatavarins and sarsapogenin</title><author>Pise, Mashitha ; Rudra, Jaishree ; Bundale, Sunita ; Begde, Deovrat ; Nashikkar, Nandita ; Upadhyay, Avinash</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-e3cf356bfe5e1e909df93660e98ec8c56bc8b56590985499dd2967b9066b0f523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>2,4-D</topic><topic>Accumulation</topic><topic>Asparagus</topic><topic>Asparagus racemosus</topic><topic>Biomass</topic><topic>Biomass production</topic><topic>Biomedical and Life Sciences</topic><topic>Callus</topic><topic>casein hydrolysates</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Cell culture techniques</topic><topic>Cultivated plants</topic><topic>Cultured cells</topic><topic>Developmental Biology</topic><topic>Growth regulators</topic><topic>Indian culture</topic><topic>Life Sciences</topic><topic>Medicinal plants</topic><topic>MOLECULAR FARMING/METABOLIC ENGINEERING/SECONDARY METABOLISM</topic><topic>naphthaleneacetic acid</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant growth regulators</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Plants</topic><topic>polygalacturonase</topic><topic>Saponins</topic><topic>steroid saponins</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pise, Mashitha</creatorcontrib><creatorcontrib>Rudra, Jaishree</creatorcontrib><creatorcontrib>Bundale, Sunita</creatorcontrib><creatorcontrib>Begde, Deovrat</creatorcontrib><creatorcontrib>Nashikkar, Nandita</creatorcontrib><creatorcontrib>Upadhyay, Avinash</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pise, Mashitha</au><au>Rudra, Jaishree</au><au>Bundale, Sunita</au><au>Begde, Deovrat</au><au>Nashikkar, Nandita</au><au>Upadhyay, Avinash</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Asparagus racemosus cell cultures: a source for enhanced production of shatavarins and sarsapogenin</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><stitle>In Vitro Cell.Dev.Biol.-Plant</stitle><date>2012-02-01</date><risdate>2012</risdate><volume>48</volume><issue>1</issue><spage>85</spage><epage>91</epage><pages>85-91</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>Asparagus racemosus is an important monocot medicinal plant that is in great demand for its steroidal saponins called shatavarins. This study was initiated to optimize the conditions for production of shatavarins in cell cultures of A. racemosus in a modified Murashige and Skoog (MS) medium supplemented with six different combinations of growth regulators. Biomass accumulation was correlated with saponin production over a 30-d culture cycle. Biomass and saponin accumulation patterns were dependent on combinations of growth regulators and the pH of the medium. Maximum levels of saponin and biomass accumulation were recorded on day 25 of the culture cycle within a pH range of 3.4 to 5.6. Total saponin produced by the in vitro cultures was 20-fold higher than amounts produced by cultivated plants. Saponin accumulation was not a biomass-associated phenomenon; cultures which showed the highest biomass accumulation were not the highest saponin accumulators. Maximum biomass (28.30 ± 0.29 g l−1) and maximum levels of shatavarin IV(11.48 ± 0.61 mg g−1) accumulation was found using a medium containing 2.0 mg l−1 2,4-D, 2 g l−1 casein hydrolysate and 0.005% pectinase. The highest levels of sarsapogenin, secreted and intracellular (4.02 ± 0.09 mg g−1), accumulated using a medium containing 1.0 mg l−1 NAA, 1.0 mg l−1 2,4-D, 0.5 mg l−1 BAP, 2 g l−1 casein hydrolysate and 0.005% pectinase, after 25 d. Shatavarins were secreted into the medium and can be isolated easily for further purification.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><doi>10.1007/s11627-011-9391-2</doi><tpages>7</tpages></addata></record> |
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| subjects | 2,4-D Accumulation Asparagus Asparagus racemosus Biomass Biomass production Biomedical and Life Sciences Callus casein hydrolysates Cell Biology Cell culture Cell culture techniques Cultivated plants Cultured cells Developmental Biology Growth regulators Indian culture Life Sciences Medicinal plants MOLECULAR FARMING/METABOLIC ENGINEERING/SECONDARY METABOLISM naphthaleneacetic acid Plant Breeding/Biotechnology Plant Genetics and Genomics Plant growth regulators Plant Physiology Plant Sciences Plants polygalacturonase Saponins steroid saponins Studies |
| title | Asparagus racemosus cell cultures: a source for enhanced production of shatavarins and sarsapogenin |
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