Evaluation of End-Capped DNA Modules for pRNA Capture and Functionalization
The ability of packaging RNA (pRNA) from the phi29 DNA packaging motor to form nanoassemblies and nanostructures has been exploited for the development of the nascent field of RNA nanotechnology and subsequent applications in nanomedicine. For applications in nanomedicine, it is necessary to modify...
Gespeichert in:
| Veröffentlicht in: | Bioconjugate chemistry 2012-04, Vol.23 (4), p.683-687 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 687 |
|---|---|
| container_issue | 4 |
| container_start_page | 683 |
| container_title | Bioconjugate chemistry |
| container_volume | 23 |
| creator | Laing, Brian M Bergstrom, Donald E |
| description | The ability of packaging RNA (pRNA) from the phi29 DNA packaging motor to form nanoassemblies and nanostructures has been exploited for the development of the nascent field of RNA nanotechnology and subsequent applications in nanomedicine. For applications in nanomedicine, it is necessary to modify the pRNA structure for the conjugation of active molecules. We have investigated end-capped double-stranded DNA segments as reversible capture reagents for pRNA. These capture agents can be designed to allow the conjugation of any desired molecule for pRNA functionalization. The results of model studies presented in this report show that 5- to 7-nucleotide overhangs on a target RNA can provide efficient handles for the high-affinity association to capped double-stranded DNA. |
| doi_str_mv | 10.1021/bc200371t |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1009187524</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2643560491</sourcerecordid><originalsourceid>FETCH-LOGICAL-a338t-d2f56e8ada16a0ac0e36abf1569a2b65d83d10ca475f00184373002d74612b723</originalsourceid><addsrcrecordid>eNpdkE1PwzAMhiMEYmNw4A-gSIgDh4LjpG12nMYGiAESgnPlNqm0qVtL0iLBryf7ACFOtuzHr-2XsVMBVwJQXOcFAshUtHusL2KESGmB-yEHJSOhAXvsyPsFAAyFxkPWQ1RKotR99jD5oKqjdl6veF3yycpEY2oaa_jN04g_1qarrOdl7XjzEgqh13bOcloZPu1WxXqOqvnXRuCYHZRUeXuyiwP2Np28ju-i2fPt_Xg0i0hK3UYGyzixmgyJhIAKsDKhvBRxMiTMk9hoaQQUpNK4BBBayVQCoElVIjBPUQ7Y-Va3cfV7Z32bLerOhTt8JjYvpjGqQF1uqcLV3jtbZo2bL8l9Bihb25b92hbYs51ily-t-SV_fArAxRagwv_d9l_oG4ZbcQ8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1009187524</pqid></control><display><type>article</type><title>Evaluation of End-Capped DNA Modules for pRNA Capture and Functionalization</title><source>ACS Publications</source><source>MEDLINE</source><creator>Laing, Brian M ; Bergstrom, Donald E</creator><creatorcontrib>Laing, Brian M ; Bergstrom, Donald E</creatorcontrib><description>The ability of packaging RNA (pRNA) from the phi29 DNA packaging motor to form nanoassemblies and nanostructures has been exploited for the development of the nascent field of RNA nanotechnology and subsequent applications in nanomedicine. For applications in nanomedicine, it is necessary to modify the pRNA structure for the conjugation of active molecules. We have investigated end-capped double-stranded DNA segments as reversible capture reagents for pRNA. These capture agents can be designed to allow the conjugation of any desired molecule for pRNA functionalization. The results of model studies presented in this report show that 5- to 7-nucleotide overhangs on a target RNA can provide efficient handles for the high-affinity association to capped double-stranded DNA.</description><identifier>ISSN: 1043-1802</identifier><identifier>EISSN: 1520-4812</identifier><identifier>DOI: 10.1021/bc200371t</identifier><identifier>PMID: 22443238</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Bacillus Phages - genetics ; Base Sequence ; Deoxyribonucleic acid ; DNA ; DNA, Viral - chemistry ; DNA, Viral - genetics ; Molecules ; Nanostructured materials ; Nanostructures - chemistry ; Nucleic Acid Conformation ; Nucleic Acid Denaturation ; Nucleic Acid Hybridization - methods ; Oligodeoxyribonucleotides - chemistry ; Oligodeoxyribonucleotides - genetics ; Ribonucleic acid ; RNA ; RNA - chemistry ; RNA - genetics ; RNA - metabolism ; Transition Temperature</subject><ispartof>Bioconjugate chemistry, 2012-04, Vol.23 (4), p.683-687</ispartof><rights>Copyright © 2012 American Chemical Society</rights><rights>2012 American Chemical Society</rights><rights>Copyright American Chemical Society Apr 18, 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a338t-d2f56e8ada16a0ac0e36abf1569a2b65d83d10ca475f00184373002d74612b723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bc200371t$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bc200371t$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22443238$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laing, Brian M</creatorcontrib><creatorcontrib>Bergstrom, Donald E</creatorcontrib><title>Evaluation of End-Capped DNA Modules for pRNA Capture and Functionalization</title><title>Bioconjugate chemistry</title><addtitle>Bioconjugate Chem</addtitle><description>The ability of packaging RNA (pRNA) from the phi29 DNA packaging motor to form nanoassemblies and nanostructures has been exploited for the development of the nascent field of RNA nanotechnology and subsequent applications in nanomedicine. For applications in nanomedicine, it is necessary to modify the pRNA structure for the conjugation of active molecules. We have investigated end-capped double-stranded DNA segments as reversible capture reagents for pRNA. These capture agents can be designed to allow the conjugation of any desired molecule for pRNA functionalization. The results of model studies presented in this report show that 5- to 7-nucleotide overhangs on a target RNA can provide efficient handles for the high-affinity association to capped double-stranded DNA.</description><subject>Bacillus Phages - genetics</subject><subject>Base Sequence</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Viral - chemistry</subject><subject>DNA, Viral - genetics</subject><subject>Molecules</subject><subject>Nanostructured materials</subject><subject>Nanostructures - chemistry</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic Acid Denaturation</subject><subject>Nucleic Acid Hybridization - methods</subject><subject>Oligodeoxyribonucleotides - chemistry</subject><subject>Oligodeoxyribonucleotides - genetics</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA - chemistry</subject><subject>RNA - genetics</subject><subject>RNA - metabolism</subject><subject>Transition Temperature</subject><issn>1043-1802</issn><issn>1520-4812</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkE1PwzAMhiMEYmNw4A-gSIgDh4LjpG12nMYGiAESgnPlNqm0qVtL0iLBryf7ACFOtuzHr-2XsVMBVwJQXOcFAshUtHusL2KESGmB-yEHJSOhAXvsyPsFAAyFxkPWQ1RKotR99jD5oKqjdl6veF3yycpEY2oaa_jN04g_1qarrOdl7XjzEgqh13bOcloZPu1WxXqOqvnXRuCYHZRUeXuyiwP2Np28ju-i2fPt_Xg0i0hK3UYGyzixmgyJhIAKsDKhvBRxMiTMk9hoaQQUpNK4BBBayVQCoElVIjBPUQ7Y-Va3cfV7Z32bLerOhTt8JjYvpjGqQF1uqcLV3jtbZo2bL8l9Bihb25b92hbYs51ily-t-SV_fArAxRagwv_d9l_oG4ZbcQ8</recordid><startdate>20120418</startdate><enddate>20120418</enddate><creator>Laing, Brian M</creator><creator>Bergstrom, Donald E</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20120418</creationdate><title>Evaluation of End-Capped DNA Modules for pRNA Capture and Functionalization</title><author>Laing, Brian M ; Bergstrom, Donald E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a338t-d2f56e8ada16a0ac0e36abf1569a2b65d83d10ca475f00184373002d74612b723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Bacillus Phages - genetics</topic><topic>Base Sequence</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Viral - chemistry</topic><topic>DNA, Viral - genetics</topic><topic>Molecules</topic><topic>Nanostructured materials</topic><topic>Nanostructures - chemistry</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic Acid Denaturation</topic><topic>Nucleic Acid Hybridization - methods</topic><topic>Oligodeoxyribonucleotides - chemistry</topic><topic>Oligodeoxyribonucleotides - genetics</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA - chemistry</topic><topic>RNA - genetics</topic><topic>RNA - metabolism</topic><topic>Transition Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laing, Brian M</creatorcontrib><creatorcontrib>Bergstrom, Donald E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bioconjugate chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laing, Brian M</au><au>Bergstrom, Donald E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of End-Capped DNA Modules for pRNA Capture and Functionalization</atitle><jtitle>Bioconjugate chemistry</jtitle><addtitle>Bioconjugate Chem</addtitle><date>2012-04-18</date><risdate>2012</risdate><volume>23</volume><issue>4</issue><spage>683</spage><epage>687</epage><pages>683-687</pages><issn>1043-1802</issn><eissn>1520-4812</eissn><abstract>The ability of packaging RNA (pRNA) from the phi29 DNA packaging motor to form nanoassemblies and nanostructures has been exploited for the development of the nascent field of RNA nanotechnology and subsequent applications in nanomedicine. For applications in nanomedicine, it is necessary to modify the pRNA structure for the conjugation of active molecules. We have investigated end-capped double-stranded DNA segments as reversible capture reagents for pRNA. These capture agents can be designed to allow the conjugation of any desired molecule for pRNA functionalization. The results of model studies presented in this report show that 5- to 7-nucleotide overhangs on a target RNA can provide efficient handles for the high-affinity association to capped double-stranded DNA.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>22443238</pmid><doi>10.1021/bc200371t</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1043-1802 |
| ispartof | Bioconjugate chemistry, 2012-04, Vol.23 (4), p.683-687 |
| issn | 1043-1802 1520-4812 |
| language | eng |
| recordid | cdi_proquest_journals_1009187524 |
| source | ACS Publications; MEDLINE |
| subjects | Bacillus Phages - genetics Base Sequence Deoxyribonucleic acid DNA DNA, Viral - chemistry DNA, Viral - genetics Molecules Nanostructured materials Nanostructures - chemistry Nucleic Acid Conformation Nucleic Acid Denaturation Nucleic Acid Hybridization - methods Oligodeoxyribonucleotides - chemistry Oligodeoxyribonucleotides - genetics Ribonucleic acid RNA RNA - chemistry RNA - genetics RNA - metabolism Transition Temperature |
| title | Evaluation of End-Capped DNA Modules for pRNA Capture and Functionalization |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T16%3A01%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20End-Capped%20DNA%20Modules%20for%20pRNA%20Capture%20and%20Functionalization&rft.jtitle=Bioconjugate%20chemistry&rft.au=Laing,%20Brian%20M&rft.date=2012-04-18&rft.volume=23&rft.issue=4&rft.spage=683&rft.epage=687&rft.pages=683-687&rft.issn=1043-1802&rft.eissn=1520-4812&rft_id=info:doi/10.1021/bc200371t&rft_dat=%3Cproquest_cross%3E2643560491%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1009187524&rft_id=info:pmid/22443238&rfr_iscdi=true |