Filamentous Soil Fungi from Ny-Ålesund, Spitsbergen, and Screening for Extracellular Enzymes
Soil filamentous fungi from Ny-Ålesund, Spitsbergen, were studied. A total of 30 fungal isolates were identified by morpho-taxonomy, and the identity of some morpho-taxonomically complex isolates was authenticated by ITS1-5.8S and ITS2 rDNA domain sequence similarity. The isolates belonged to 19 spe...
Gespeichert in:
| Veröffentlicht in: | Arctic 2012-03, Vol.65 (1), p.45-55 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 55 |
|---|---|
| container_issue | 1 |
| container_start_page | 45 |
| container_title | Arctic |
| container_volume | 65 |
| creator | SINGH, SHIV M. SINGH, SANJAY K. YADAV, LAL S. SINGH, PARAS N. RAVINDRA, RASIK |
| description | Soil filamentous fungi from Ny-Ålesund, Spitsbergen, were studied. A total of 30 fungal isolates were identified by morpho-taxonomy, and the identity of some morpho-taxonomically complex isolates was authenticated by ITS1-5.8S and ITS2 rDNA domain sequence similarity. The isolates belonged to 19 species under 14 genera (Acremonium, Arthrinium, Aspergillus, Cladosporium, Corynespora, Emericella, Geomyces, Mortierella, Mucor, Myrothecium, Penicillium, Phialophora, Preussia, Xylaria). To the best of our knowledge, Acremonium roseolum, Aspergillus aculeatus, Emericella nidulans, and Preussia sp. are the first northernmost records from Arctic soils. The viable fungal count in different soil samples varied from 0.5 × 104 to 2.0 × 105 g-1. Species richness in different soil samples was also calculated. Mortierella was one of the most dominant genera in Arctic soils. A temperature tolerance study was carried out for all the isolates, and representative species were screened for their extracellular enzyme activity (amylase, cellulase, phosphatase, and pectinase) at 4°C and 20°C. Among the 30 isolates, seven showed cellulolytic activity, two were phosphate solubilizers, three had amylolytic activity, and only one showed pectinolytic activity on solid media. CMCase (β1, 4-endoglucanase) activity was quantified in seven isolates that exhibited positive activity during preliminary screening. The records of enzyme activity for amylases, pectinases, and cellulases are the first from the fungi of Spitsbergen. The present study indicates the dominance in Ny-Ålesund of cellulolytic strains, which may serve as potent decomposers in Arctic tundra. These isolates may be used to facilitate the mineralization of cellulolytic wastes generated by human activities in colder hilly areas across the world, including the Himalayas in India. Nous avons étudié des champignons telluriques filamenteux de Ny-Ålesund, Spitzberg. Grâce à la morphotaxonomie, nous avons identifié 30 isolats fongiques, et l'identité de certains complexes d'isolats morpho-taxonomiques a été authentifiée au moyen des similarités des séquences de domaines ITS1-5.8S et ITS2 DNAr. Les isolats relevaient de 19 espèces faisant partie de 14 genres (Acremonium, Arthrinium, Aspergillus, Cladosporium, Corynespora, Emericella, Geomyces, Mortierella, Mucor, Myrothecium, Penicillium, Phialophora, Preussia, Xylaria). Au meilleur de nos connaissances, Acremonium roseolum, Aspergillus aculeatus, Emericella nidulans et Preussia s |
| doi_str_mv | 10.14430/arctic4164 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_1000456202</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A288875448</galeid><jstor_id>23187223</jstor_id><sourcerecordid>A288875448</sourcerecordid><originalsourceid>FETCH-LOGICAL-c517t-d805c7de9c7103a5c4a365f10c8f721c714075515cf153b02c5286c45bc417b33</originalsourceid><addsrcrecordid>eNqV0k9rFDEUAPAgCq61J89C0FOx0-bvZnospauLpYqrRwnZTDJkySTbJANd734yv5ipK20X9qDkEPL45b288AB4hdEJZoyiU5V0cZrhKXsCJviM0AYTxp-CCUKINahl9Dl4kfMKIYywQBPwfea8GkwoccxwEZ2HszH0DtoUB3i9aX799CaPoTuGi7UreWlSb8IxVKGDC52MCS700MYEL29LUtp4P3pVT-HHZjD5JXhmlc_m8O9-AL7NLr9efGiuPr2fX5xfNZpjUZquRVyLzpxpgRFVXDNFp9xipFsrCK5RhgTnmGuLOV0iojlpp5rxZW1VLCk9AG-3edcp3owmF7mKYwq1pMR3nfMpQeRB9cob6YKNd08eXNbynLRtKzhjbVXNHlW7Nkn5GIx1Nbzj3-zxeu1u5GN0sgfV1ZnB6b1Zj3YuVFPMbenVmLP8-Hn-z3a--PIf9nrXvttanWLOyVi5Tm5QaVM_Vf6ZN_kwb1W_3upVLjHdU0JxKwih9DeXFszP</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1000456202</pqid></control><display><type>article</type><title>Filamentous Soil Fungi from Ny-Ålesund, Spitsbergen, and Screening for Extracellular Enzymes</title><source>Jstor Complete Legacy</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>SINGH, SHIV M. ; SINGH, SANJAY K. ; YADAV, LAL S. ; SINGH, PARAS N. ; RAVINDRA, RASIK</creator><creatorcontrib>SINGH, SHIV M. ; SINGH, SANJAY K. ; YADAV, LAL S. ; SINGH, PARAS N. ; RAVINDRA, RASIK</creatorcontrib><description>Soil filamentous fungi from Ny-Ålesund, Spitsbergen, were studied. A total of 30 fungal isolates were identified by morpho-taxonomy, and the identity of some morpho-taxonomically complex isolates was authenticated by ITS1-5.8S and ITS2 rDNA domain sequence similarity. The isolates belonged to 19 species under 14 genera (Acremonium, Arthrinium, Aspergillus, Cladosporium, Corynespora, Emericella, Geomyces, Mortierella, Mucor, Myrothecium, Penicillium, Phialophora, Preussia, Xylaria). To the best of our knowledge, Acremonium roseolum, Aspergillus aculeatus, Emericella nidulans, and Preussia sp. are the first northernmost records from Arctic soils. The viable fungal count in different soil samples varied from 0.5 × 104 to 2.0 × 105 g-1. Species richness in different soil samples was also calculated. Mortierella was one of the most dominant genera in Arctic soils. A temperature tolerance study was carried out for all the isolates, and representative species were screened for their extracellular enzyme activity (amylase, cellulase, phosphatase, and pectinase) at 4°C and 20°C. Among the 30 isolates, seven showed cellulolytic activity, two were phosphate solubilizers, three had amylolytic activity, and only one showed pectinolytic activity on solid media. CMCase (β1, 4-endoglucanase) activity was quantified in seven isolates that exhibited positive activity during preliminary screening. The records of enzyme activity for amylases, pectinases, and cellulases are the first from the fungi of Spitsbergen. The present study indicates the dominance in Ny-Ålesund of cellulolytic strains, which may serve as potent decomposers in Arctic tundra. These isolates may be used to facilitate the mineralization of cellulolytic wastes generated by human activities in colder hilly areas across the world, including the Himalayas in India. Nous avons étudié des champignons telluriques filamenteux de Ny-Ålesund, Spitzberg. Grâce à la morphotaxonomie, nous avons identifié 30 isolats fongiques, et l'identité de certains complexes d'isolats morpho-taxonomiques a été authentifiée au moyen des similarités des séquences de domaines ITS1-5.8S et ITS2 DNAr. Les isolats relevaient de 19 espèces faisant partie de 14 genres (Acremonium, Arthrinium, Aspergillus, Cladosporium, Corynespora, Emericella, Geomyces, Mortierella, Mucor, Myrothecium, Penicillium, Phialophora, Preussia, Xylaria). Au meilleur de nos connaissances, Acremonium roseolum, Aspergillus aculeatus, Emericella nidulans et Preussia sp. constituent les premiers enregistrements aussi nordiques des sols arctiques. Le dénombrement viable de champignons dans différents échantillons de sol variait de 0,5 × 104 à 2,0 × 105 g-1. Nous avons également calculé la diversité des espèces prélevées dans différents échantillons de sol. Le genre Mortierella était l'un des plus dominants des sols arctiques. Nous avons étudié la tolérance à la température de tous les isolats, et des espèces représentatives ont été examinées du point de vue de l'activité enzymatique extracellulaire (amylase, cellulase, phosphatase et pectinase) à 4 °C et 20 °C. Parmi les 30 isolats, sept présentaient de l'activité cellulolytique, deux étaient des solubilisants du phosphate, trois présentaient de l'activité amylolytique et seulement un présentait de l'activité pectolytique dans le cas des solides. L'activité CMCase (β1, 4-endoglucanase) a été quantifiée dans sept isolats qui affichaient une activité positive au cours de l'examen préliminaire. Il s'agissait de la première fois que de l'activité enzymatique pour les amylases, pectinases et cellulases a été détectée dans les champignons de Spitzberg. Cette étude indique la dominance de souches cellulolytiques à Ny-Ålesund, souches qui peuvent servir de décomposeurs puissants dans la toundra arctique. Ces isolats peuvent servir à faciliter la minéralisation des déchets cellulolytiques émanant des activités humaines dans les régions montagneuses plus froides du monde entier, y compris l'Himalaya, en Inde.</description><identifier>ISSN: 0004-0843</identifier><identifier>EISSN: 1923-1245</identifier><identifier>DOI: 10.14430/arctic4164</identifier><language>eng</language><publisher>Calgary: The Arctic Institute of North America</publisher><subject>Acid soils ; Aspergillus ; Enzymes ; Fungi ; Mortierella ; Organic soils ; Soil ecology ; Soil fungi ; Soil samples ; Soil science ; Soil sciences ; Studies ; Taxonomy</subject><ispartof>Arctic, 2012-03, Vol.65 (1), p.45-55</ispartof><rights>Copyright Canada 2012 Arctic Institute of North America</rights><rights>COPYRIGHT 2012 Arctic Institute of North America of the University of Calgary</rights><rights>Copyright Arctic Institute of North America Mar 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-d805c7de9c7103a5c4a365f10c8f721c714075515cf153b02c5286c45bc417b33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23187223$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23187223$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,57995,58228</link.rule.ids></links><search><creatorcontrib>SINGH, SHIV M.</creatorcontrib><creatorcontrib>SINGH, SANJAY K.</creatorcontrib><creatorcontrib>YADAV, LAL S.</creatorcontrib><creatorcontrib>SINGH, PARAS N.</creatorcontrib><creatorcontrib>RAVINDRA, RASIK</creatorcontrib><title>Filamentous Soil Fungi from Ny-Ålesund, Spitsbergen, and Screening for Extracellular Enzymes</title><title>Arctic</title><description>Soil filamentous fungi from Ny-Ålesund, Spitsbergen, were studied. A total of 30 fungal isolates were identified by morpho-taxonomy, and the identity of some morpho-taxonomically complex isolates was authenticated by ITS1-5.8S and ITS2 rDNA domain sequence similarity. The isolates belonged to 19 species under 14 genera (Acremonium, Arthrinium, Aspergillus, Cladosporium, Corynespora, Emericella, Geomyces, Mortierella, Mucor, Myrothecium, Penicillium, Phialophora, Preussia, Xylaria). To the best of our knowledge, Acremonium roseolum, Aspergillus aculeatus, Emericella nidulans, and Preussia sp. are the first northernmost records from Arctic soils. The viable fungal count in different soil samples varied from 0.5 × 104 to 2.0 × 105 g-1. Species richness in different soil samples was also calculated. Mortierella was one of the most dominant genera in Arctic soils. A temperature tolerance study was carried out for all the isolates, and representative species were screened for their extracellular enzyme activity (amylase, cellulase, phosphatase, and pectinase) at 4°C and 20°C. Among the 30 isolates, seven showed cellulolytic activity, two were phosphate solubilizers, three had amylolytic activity, and only one showed pectinolytic activity on solid media. CMCase (β1, 4-endoglucanase) activity was quantified in seven isolates that exhibited positive activity during preliminary screening. The records of enzyme activity for amylases, pectinases, and cellulases are the first from the fungi of Spitsbergen. The present study indicates the dominance in Ny-Ålesund of cellulolytic strains, which may serve as potent decomposers in Arctic tundra. These isolates may be used to facilitate the mineralization of cellulolytic wastes generated by human activities in colder hilly areas across the world, including the Himalayas in India. Nous avons étudié des champignons telluriques filamenteux de Ny-Ålesund, Spitzberg. Grâce à la morphotaxonomie, nous avons identifié 30 isolats fongiques, et l'identité de certains complexes d'isolats morpho-taxonomiques a été authentifiée au moyen des similarités des séquences de domaines ITS1-5.8S et ITS2 DNAr. Les isolats relevaient de 19 espèces faisant partie de 14 genres (Acremonium, Arthrinium, Aspergillus, Cladosporium, Corynespora, Emericella, Geomyces, Mortierella, Mucor, Myrothecium, Penicillium, Phialophora, Preussia, Xylaria). Au meilleur de nos connaissances, Acremonium roseolum, Aspergillus aculeatus, Emericella nidulans et Preussia sp. constituent les premiers enregistrements aussi nordiques des sols arctiques. Le dénombrement viable de champignons dans différents échantillons de sol variait de 0,5 × 104 à 2,0 × 105 g-1. Nous avons également calculé la diversité des espèces prélevées dans différents échantillons de sol. Le genre Mortierella était l'un des plus dominants des sols arctiques. Nous avons étudié la tolérance à la température de tous les isolats, et des espèces représentatives ont été examinées du point de vue de l'activité enzymatique extracellulaire (amylase, cellulase, phosphatase et pectinase) à 4 °C et 20 °C. Parmi les 30 isolats, sept présentaient de l'activité cellulolytique, deux étaient des solubilisants du phosphate, trois présentaient de l'activité amylolytique et seulement un présentait de l'activité pectolytique dans le cas des solides. L'activité CMCase (β1, 4-endoglucanase) a été quantifiée dans sept isolats qui affichaient une activité positive au cours de l'examen préliminaire. Il s'agissait de la première fois que de l'activité enzymatique pour les amylases, pectinases et cellulases a été détectée dans les champignons de Spitzberg. Cette étude indique la dominance de souches cellulolytiques à Ny-Ålesund, souches qui peuvent servir de décomposeurs puissants dans la toundra arctique. Ces isolats peuvent servir à faciliter la minéralisation des déchets cellulolytiques émanant des activités humaines dans les régions montagneuses plus froides du monde entier, y compris l'Himalaya, en Inde.</description><subject>Acid soils</subject><subject>Aspergillus</subject><subject>Enzymes</subject><subject>Fungi</subject><subject>Mortierella</subject><subject>Organic soils</subject><subject>Soil ecology</subject><subject>Soil fungi</subject><subject>Soil samples</subject><subject>Soil science</subject><subject>Soil sciences</subject><subject>Studies</subject><subject>Taxonomy</subject><issn>0004-0843</issn><issn>1923-1245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>KPI</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqV0k9rFDEUAPAgCq61J89C0FOx0-bvZnospauLpYqrRwnZTDJkySTbJANd734yv5ipK20X9qDkEPL45b288AB4hdEJZoyiU5V0cZrhKXsCJviM0AYTxp-CCUKINahl9Dl4kfMKIYywQBPwfea8GkwoccxwEZ2HszH0DtoUB3i9aX799CaPoTuGi7UreWlSb8IxVKGDC52MCS700MYEL29LUtp4P3pVT-HHZjD5JXhmlc_m8O9-AL7NLr9efGiuPr2fX5xfNZpjUZquRVyLzpxpgRFVXDNFp9xipFsrCK5RhgTnmGuLOV0iojlpp5rxZW1VLCk9AG-3edcp3owmF7mKYwq1pMR3nfMpQeRB9cob6YKNd08eXNbynLRtKzhjbVXNHlW7Nkn5GIx1Nbzj3-zxeu1u5GN0sgfV1ZnB6b1Zj3YuVFPMbenVmLP8-Hn-z3a--PIf9nrXvttanWLOyVi5Tm5QaVM_Vf6ZN_kwb1W_3upVLjHdU0JxKwih9DeXFszP</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>SINGH, SHIV M.</creator><creator>SINGH, SANJAY K.</creator><creator>YADAV, LAL S.</creator><creator>SINGH, PARAS N.</creator><creator>RAVINDRA, RASIK</creator><general>The Arctic Institute of North America</general><general>Arctic Institute of North America of the University of Calgary</general><general>Arctic Institute of North America</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ISN</scope><scope>ISR</scope><scope>KPI</scope><scope>3V.</scope><scope>7ST</scope><scope>7TG</scope><scope>7TN</scope><scope>7XB</scope><scope>8FE</scope><scope>8FG</scope><scope>8FK</scope><scope>8FQ</scope><scope>8FV</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>GNUQQ</scope><scope>H95</scope><scope>HCIFZ</scope><scope>KL.</scope><scope>L.G</scope><scope>L6V</scope><scope>M3G</scope><scope>M7S</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>S0X</scope><scope>SOI</scope></search><sort><creationdate>20120301</creationdate><title>Filamentous Soil Fungi from Ny-Ålesund, Spitsbergen, and Screening for Extracellular Enzymes</title><author>SINGH, SHIV M. ; SINGH, SANJAY K. ; YADAV, LAL S. ; SINGH, PARAS N. ; RAVINDRA, RASIK</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-d805c7de9c7103a5c4a365f10c8f721c714075515cf153b02c5286c45bc417b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acid soils</topic><topic>Aspergillus</topic><topic>Enzymes</topic><topic>Fungi</topic><topic>Mortierella</topic><topic>Organic soils</topic><topic>Soil ecology</topic><topic>Soil fungi</topic><topic>Soil samples</topic><topic>Soil science</topic><topic>Soil sciences</topic><topic>Studies</topic><topic>Taxonomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SINGH, SHIV M.</creatorcontrib><creatorcontrib>SINGH, SANJAY K.</creatorcontrib><creatorcontrib>YADAV, LAL S.</creatorcontrib><creatorcontrib>SINGH, PARAS N.</creatorcontrib><creatorcontrib>RAVINDRA, RASIK</creatorcontrib><collection>CrossRef</collection><collection>Gale In Context: Canada</collection><collection>Gale In Context: Science</collection><collection>Gale In Context: Global Issues</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Oceanic Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Canadian Business & Current Affairs Database</collection><collection>Canadian Business & Current Affairs Database (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>SciTech Premium Collection</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Engineering Collection</collection><collection>CBCA Reference & Current Events</collection><collection>Engineering Database</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>Environment Abstracts</collection><jtitle>Arctic</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SINGH, SHIV M.</au><au>SINGH, SANJAY K.</au><au>YADAV, LAL S.</au><au>SINGH, PARAS N.</au><au>RAVINDRA, RASIK</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Filamentous Soil Fungi from Ny-Ålesund, Spitsbergen, and Screening for Extracellular Enzymes</atitle><jtitle>Arctic</jtitle><date>2012-03-01</date><risdate>2012</risdate><volume>65</volume><issue>1</issue><spage>45</spage><epage>55</epage><pages>45-55</pages><issn>0004-0843</issn><eissn>1923-1245</eissn><abstract>Soil filamentous fungi from Ny-Ålesund, Spitsbergen, were studied. A total of 30 fungal isolates were identified by morpho-taxonomy, and the identity of some morpho-taxonomically complex isolates was authenticated by ITS1-5.8S and ITS2 rDNA domain sequence similarity. The isolates belonged to 19 species under 14 genera (Acremonium, Arthrinium, Aspergillus, Cladosporium, Corynespora, Emericella, Geomyces, Mortierella, Mucor, Myrothecium, Penicillium, Phialophora, Preussia, Xylaria). To the best of our knowledge, Acremonium roseolum, Aspergillus aculeatus, Emericella nidulans, and Preussia sp. are the first northernmost records from Arctic soils. The viable fungal count in different soil samples varied from 0.5 × 104 to 2.0 × 105 g-1. Species richness in different soil samples was also calculated. Mortierella was one of the most dominant genera in Arctic soils. A temperature tolerance study was carried out for all the isolates, and representative species were screened for their extracellular enzyme activity (amylase, cellulase, phosphatase, and pectinase) at 4°C and 20°C. Among the 30 isolates, seven showed cellulolytic activity, two were phosphate solubilizers, three had amylolytic activity, and only one showed pectinolytic activity on solid media. CMCase (β1, 4-endoglucanase) activity was quantified in seven isolates that exhibited positive activity during preliminary screening. The records of enzyme activity for amylases, pectinases, and cellulases are the first from the fungi of Spitsbergen. The present study indicates the dominance in Ny-Ålesund of cellulolytic strains, which may serve as potent decomposers in Arctic tundra. These isolates may be used to facilitate the mineralization of cellulolytic wastes generated by human activities in colder hilly areas across the world, including the Himalayas in India. Nous avons étudié des champignons telluriques filamenteux de Ny-Ålesund, Spitzberg. Grâce à la morphotaxonomie, nous avons identifié 30 isolats fongiques, et l'identité de certains complexes d'isolats morpho-taxonomiques a été authentifiée au moyen des similarités des séquences de domaines ITS1-5.8S et ITS2 DNAr. Les isolats relevaient de 19 espèces faisant partie de 14 genres (Acremonium, Arthrinium, Aspergillus, Cladosporium, Corynespora, Emericella, Geomyces, Mortierella, Mucor, Myrothecium, Penicillium, Phialophora, Preussia, Xylaria). Au meilleur de nos connaissances, Acremonium roseolum, Aspergillus aculeatus, Emericella nidulans et Preussia sp. constituent les premiers enregistrements aussi nordiques des sols arctiques. Le dénombrement viable de champignons dans différents échantillons de sol variait de 0,5 × 104 à 2,0 × 105 g-1. Nous avons également calculé la diversité des espèces prélevées dans différents échantillons de sol. Le genre Mortierella était l'un des plus dominants des sols arctiques. Nous avons étudié la tolérance à la température de tous les isolats, et des espèces représentatives ont été examinées du point de vue de l'activité enzymatique extracellulaire (amylase, cellulase, phosphatase et pectinase) à 4 °C et 20 °C. Parmi les 30 isolats, sept présentaient de l'activité cellulolytique, deux étaient des solubilisants du phosphate, trois présentaient de l'activité amylolytique et seulement un présentait de l'activité pectolytique dans le cas des solides. L'activité CMCase (β1, 4-endoglucanase) a été quantifiée dans sept isolats qui affichaient une activité positive au cours de l'examen préliminaire. Il s'agissait de la première fois que de l'activité enzymatique pour les amylases, pectinases et cellulases a été détectée dans les champignons de Spitzberg. Cette étude indique la dominance de souches cellulolytiques à Ny-Ålesund, souches qui peuvent servir de décomposeurs puissants dans la toundra arctique. Ces isolats peuvent servir à faciliter la minéralisation des déchets cellulolytiques émanant des activités humaines dans les régions montagneuses plus froides du monde entier, y compris l'Himalaya, en Inde.</abstract><cop>Calgary</cop><pub>The Arctic Institute of North America</pub><doi>10.14430/arctic4164</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0004-0843 |
| ispartof | Arctic, 2012-03, Vol.65 (1), p.45-55 |
| issn | 0004-0843 1923-1245 |
| language | eng |
| recordid | cdi_proquest_journals_1000456202 |
| source | Jstor Complete Legacy; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acid soils Aspergillus Enzymes Fungi Mortierella Organic soils Soil ecology Soil fungi Soil samples Soil science Soil sciences Studies Taxonomy |
| title | Filamentous Soil Fungi from Ny-Ålesund, Spitsbergen, and Screening for Extracellular Enzymes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T07%3A49%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Filamentous%20Soil%20Fungi%20from%20Ny-%C3%85lesund,%20Spitsbergen,%20and%20Screening%20for%20Extracellular%20Enzymes&rft.jtitle=Arctic&rft.au=SINGH,%20SHIV%20M.&rft.date=2012-03-01&rft.volume=65&rft.issue=1&rft.spage=45&rft.epage=55&rft.pages=45-55&rft.issn=0004-0843&rft.eissn=1923-1245&rft_id=info:doi/10.14430/arctic4164&rft_dat=%3Cgale_proqu%3EA288875448%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1000456202&rft_id=info:pmid/&rft_galeid=A288875448&rft_jstor_id=23187223&rfr_iscdi=true |