Metabolite Profiling of Mammalian Cells

As an integrative readout of cellular status, the metabolome (metabolite profile) of cells and medium can offer a powerful (and, potentially, noninvasive) indication of cell status, providing information of molecular events that determine, regulate, or limit specific cell functions including the capacity to support recombinant protein production. This chapter focuses on the manner in which information about the metabolite status of mammalian cells can be collected, interpreted, and used as a platform to optimize the capability to manufacture commercially valuable recombinant proteins in industrially relevant mammalian cell lines.