A Peptide-Based Fluorescence Resonance Energy Transfer Assay for Bacillus anthracis Lethal Factor Protease

A fluorescence resonance energy transfer assay has been developed for monitoring Bacillus anthracis lethal factor (LF) protease activity. A fluorogenic 16-mer peptide based on the known LF protease substrate MEK1 was synthesized and found to be cleaved by the enzyme at the anticipated site. Extensio...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2002-05, Vol.99 (10), p.6603-6606
Hauptverfasser:	Cummings, Richard T., Salowe, Scott P., Cunningham, Barry R., Wiltsie, Judyann, Park, Young Whan, Sonatore, Lisa M., Wisniewski, Douglas, Douglas, Cameron M., Hermes, Jeffrey D., Scolnick, Edward M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Anthrax Antigens, Bacterial Bacillus anthracis Bacillus anthracis - enzymology Bacterial Toxins - metabolism Biochemistry Biological Sciences Consensus sequence Energy transfer Enzymes Fluorescence lethal factor MAP Kinase Kinase 1 Medical schools Metalloendopeptidases - metabolism Mitogen-Activated Protein Kinase Kinases - metabolism Oxidation Peptides Peptides - metabolism Protein-Serine-Threonine Kinases - metabolism Resins Spectrometry, Fluorescence - methods Spectrophotometry, Ultraviolet - methods Spectroscopy Studies Substrate Specificity Toxins
Online-Zugang:	Volltext
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