A DNA Transfection System for Generation of Influenza a Virus from Eight Plasmids

We have developed an eight-plasmid DNA transfection system for the rescue of infectious influenza A virus from cloned cDNA. In this plasmid-based expression system, viral cDNA is inserted between the RNA polymerase I (pol I) promoter and terminator sequences. This entire pol I transcription unit is...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2000-05, Vol.97 (11), p.6108-6113
Hauptverfasser:	Hoffmann, Erich, Neumann, Gabriele, Kawaoka, Yoshihiro, Hobom, Gerd, Webster, Robert G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological Sciences Cell Line Cell lines Complementary DNA Deoxyribonucleic acid DNA DNA, Complementary - genetics DNA, Viral - genetics Dogs Humans Influenza Influenza A virus Influenza A virus - genetics Influenza A virus - growth & development Kidney cells Messenger RNA Microbiology Molecular Sequence Data Plasmids Plasmids - genetics Promoter Regions, Genetic Recombination, Genetic Regulatory Sequences, Nucleic Acid RNA RNA Polymerase I - genetics RNA Polymerase II - genetics RNA, Messenger - biosynthesis RNA, Viral - biosynthesis Transcription, Genetic Transfection Transfection - methods Viruses
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