The Cys4 zinc finger of bacteriophage T7 primase in sequence-specific single-stranded DNA recognition

Bacteriophage T7 DNA primase recognizes 5′-GTC-3′ in single-stranded DNA. The primase contains a single Cys 4 zinc-binding motif that is essential for recognition. Biochemical and mutagenic analyses suggest that the Cys 4 motif contacts cytosine of 5′-GTC-3′ and may also contribute to thymine recogn...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1999-04, Vol.96 (8), p.4295-4300
Hauptverfasser:	Kusakabe, T, Hine, A V, Hyberts, S G, Richardson, C C
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteriophage T7 - enzymology Base Sequence Binding Sites Biochemistry Biological Sciences Cysteine Deoxyribonucleic acid DNA DNA Primase - chemistry DNA Primase - metabolism DNA, Single-Stranded - chemistry DNA, Single-Stranded - metabolism Enzymes Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Nucleic Acid Conformation Oligodeoxyribonucleotides - chemical synthesis Oligodeoxyribonucleotides - chemistry Oligodeoxyribonucleotides - metabolism Protein Conformation Recombinant Proteins - chemistry Recombinant Proteins - metabolism Thymine Transcription Factors - chemistry Transcription Factors - metabolism Transcription Factors, General Transcriptional Elongation Factors Zinc Zinc Fingers
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