Mechanism by Which the IFN-β Enhanceosome Activates Transcription
We demonstrate that in contrast to previous findings by using simple synthetic promoters or activators, the natural IFN-β enhanceosome activates transcription by causing a dramatic increase of the rate by which preinitiation complexes assemble at the promoter. This effect totally depends on the recr...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1999-11, Vol.96 (23), p.13108-13113 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Yie, Junming Senger, Kate Thanos, Dimitris |
| description | We demonstrate that in contrast to previous findings by using simple synthetic promoters or activators, the natural IFN-β enhanceosome activates transcription by causing a dramatic increase of the rate by which preinitiation complexes assemble at the promoter. This effect totally depends on the recruitment of the CBP-PolII holoenzyme by the enhanceosome, because its depletion from the extract decelerates the rate of transcription. However, addition of the CBP-PolII holoenzyme back to these extracts fully restores the speed by which the enhanceosome activates transcription. Strikingly, preincubation of the enhanceosome with the CBP-RNA PolII holoenzyme complex results in instant assembly of preinitiation complexes. In contrast, individual IFN-β gene activators function solely by increasing the number of functional preinitiation complexes and not the rate of their assembly. Thus, fast recruitment of the CBP-RNA PolII holoenzyme complex is critical for the rapid activation of IFN-β gene expression by virus infection. |
| doi_str_mv | 10.1073/pnas.96.23.13108 |
| format | Article |
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This effect totally depends on the recruitment of the CBP-PolII holoenzyme by the enhanceosome, because its depletion from the extract decelerates the rate of transcription. However, addition of the CBP-PolII holoenzyme back to these extracts fully restores the speed by which the enhanceosome activates transcription. Strikingly, preincubation of the enhanceosome with the CBP-RNA PolII holoenzyme complex results in instant assembly of preinitiation complexes. In contrast, individual IFN-β gene activators function solely by increasing the number of functional preinitiation complexes and not the rate of their assembly. 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This effect totally depends on the recruitment of the CBP-PolII holoenzyme by the enhanceosome, because its depletion from the extract decelerates the rate of transcription. However, addition of the CBP-PolII holoenzyme back to these extracts fully restores the speed by which the enhanceosome activates transcription. Strikingly, preincubation of the enhanceosome with the CBP-RNA PolII holoenzyme complex results in instant assembly of preinitiation complexes. In contrast, individual IFN-β gene activators function solely by increasing the number of functional preinitiation complexes and not the rate of their assembly. Thus, fast recruitment of the CBP-RNA PolII holoenzyme complex is critical for the rapid activation of IFN-β gene expression by virus infection.</description><subject>Antibodies</subject><subject>b-Interferon</subject><subject>Biochemistry</subject><subject>Biological Sciences</subject><subject>CBP protein</subject><subject>DNA</subject><subject>enhanceosomes</subject><subject>Enhancer Elements, Genetic</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Genes</subject><subject>HeLa Cells</subject><subject>Histones</subject><subject>Humans</subject><subject>Infections</subject><subject>Interferon-beta - genetics</subject><subject>Interferon-beta - physiology</subject><subject>Kinetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Promoter regions</subject><subject>Promoter Regions, Genetic</subject><subject>RNA</subject><subject>RNA Polymerase III - metabolism</subject><subject>Trans-Activators - metabolism</subject><subject>Transcriptional Activation - physiology</subject><subject>Viruses</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EokvhjjhAxAFxyTJjJ_4jcSlVC5UKXIo4Wo7jkKySeGs7Ff1afBA-Ewm7VC0HOM3h_d7ozTxCniKsEQR7sx1NXCu-pmyNDEHeIysEhTkvFNwnKwAqclnQ4oA8inEDAKqU8JAcIJSloBJX5N1HZ1szdnHIquvsa9vZNkuty85OP-U_f2Qn4yxa56MfXHZkU3dlkovZRTBjtKHbps6Pj8mDxvTRPdnPQ_Ll9OTi-EN-_vn92fHReW5LWqS8YoAcheNlgdIVzJamqQVIzurGUIU456RGWC6LqkLFa2uk4lVjLKCta8kOydvd3u1UDa62bkzB9HobusGEa-1Np-8qY9fqb_5KU6Zgsb_a24O_nFxMeuiidX1vRuenqLmigkvG_guiKKDEcgFf_gVu_BTG-QeaAjKJnIkZgh1kg48xuOYmMIJeStRLiVrxOab-XeJseX770FuGXWsz8GIPLNY_8t0Vr_9N6Gbq--S-pxl9tkM3MflwwxYKKWe_AKc1ufQ</recordid><startdate>19991109</startdate><enddate>19991109</enddate><creator>Yie, Junming</creator><creator>Senger, Kate</creator><creator>Thanos, Dimitris</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19991109</creationdate><title>Mechanism by Which the IFN-β Enhanceosome Activates Transcription</title><author>Yie, Junming ; 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This effect totally depends on the recruitment of the CBP-PolII holoenzyme by the enhanceosome, because its depletion from the extract decelerates the rate of transcription. However, addition of the CBP-PolII holoenzyme back to these extracts fully restores the speed by which the enhanceosome activates transcription. Strikingly, preincubation of the enhanceosome with the CBP-RNA PolII holoenzyme complex results in instant assembly of preinitiation complexes. In contrast, individual IFN-β gene activators function solely by increasing the number of functional preinitiation complexes and not the rate of their assembly. Thus, fast recruitment of the CBP-RNA PolII holoenzyme complex is critical for the rapid activation of IFN-β gene expression by virus infection.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>10557281</pmid><doi>10.1073/pnas.96.23.13108</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies b-Interferon Biochemistry Biological Sciences CBP protein DNA enhanceosomes Enhancer Elements, Genetic Enzymes Gene expression Genes HeLa Cells Histones Humans Infections Interferon-beta - genetics Interferon-beta - physiology Kinetics Nuclear Proteins - metabolism Promoter regions Promoter Regions, Genetic RNA RNA Polymerase III - metabolism Trans-Activators - metabolism Transcriptional Activation - physiology Viruses |
| title | Mechanism by Which the IFN-β Enhanceosome Activates Transcription |
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