Single-copy transgenic wheat generated through the resolution of complex integration patterns
Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flank...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1999-09, Vol.96 (20), p.11117-11121 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Srivastava, V Anderson, O.D Ow, D.W |
| description | Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flanked by recombination sites in an inverted orientation. Regardless of the number of copies integrated between the outermost transgenes, recombination between the outermost sites resolves the integrated molecules into a single copy. An example of this strategy has been demonstrated with wheat transformation, where four of four multiple-copy loci were resolved successfully into single-copy transgenes. |
| doi_str_mv | 10.1073/pnas.96.20.11117 |
| format | Article |
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To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flanked by recombination sites in an inverted orientation. Regardless of the number of copies integrated between the outermost transgenes, recombination between the outermost sites resolves the integrated molecules into a single copy. 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To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flanked by recombination sites in an inverted orientation. Regardless of the number of copies integrated between the outermost transgenes, recombination between the outermost sites resolves the integrated molecules into a single copy. An example of this strategy has been demonstrated with wheat transformation, where four of four multiple-copy loci were resolved successfully into single-copy transgenes.</description><subject>alleles</subject><subject>bar gene</subject><subject>bilanafos</subject><subject>biolistics</subject><subject>Biological Sciences</subject><subject>Chimera</subject><subject>Chromosome Inversion</subject><subject>cre locus</subject><subject>cre recombinase</subject><subject>DNA</subject><subject>DNA probes</subject><subject>Embryos</subject><subject>Gene Dosage</subject><subject>gene transfer</subject><subject>genes</subject><subject>Genetic loci</subject><subject>genetic recombination</subject><subject>genetic transformation</subject><subject>Genetic Vectors</subject><subject>Genomics</subject><subject>herbicide resistance</subject><subject>loci</subject><subject>lox allele</subject><subject>nucleases</subject><subject>Nucleic acid hybridization</subject><subject>Plants</subject><subject>Plants, Genetically Modified</subject><subject>plasmid vectors</subject><subject>Recombination, Genetic</subject><subject>reporter genes</subject><subject>segregation</subject><subject>single-copy transgenes</subject><subject>site-specific recombination</subject><subject>Transformation, Genetic</subject><subject>Transgenes</subject><subject>Transgenic plants</subject><subject>Triticum - genetics</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EokvhjjhATohLlnH8kbXEBVV8SZU4lB6R5XUm2VReO9gOtP8eR7tU2wu-jMfzvDNjvYS8pLCm0LL3kzdpreS6KXk57SOyoqBoLbmCx2QF0LT1hjf8jDxL6QYAlNjAU3JGQQBQplbk59XoB4e1DdNdlaPxaUA_2urPDk2uyh2jydhVeRfDPOxKxCpiCm7OY_BV6Csb9pPD22r0GYcCL8-TyRmjT8_Jk964hC-O8Zxcf_704-Jrffn9y7eLj5e1FULmmlHRWSuRioZa0UlusYVOUAq4sQYEw5aDFGCh79ByIZjhWyZpw6zFjm3ZOflw6DvN2z12Fn35itNTHPcm3ulgRv2w4sedHsJvTVulZJG_Pcpj-DVjyno_JovOGY9hTrqFVrWsgQLCAbQxpBSxvx9BQS-O6MURraRuSr44UiSvT1c7ERwsKMCbI7BI_5Uftnj3f0L3s3MZb3NBXx3Qm5RDvGf5RqmTSb0J2gxxTPr6qilLQKOE5Jyxv79btVw</recordid><startdate>19990928</startdate><enddate>19990928</enddate><creator>Srivastava, V</creator><creator>Anderson, O.D</creator><creator>Ow, D.W</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>The National Academy of Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19990928</creationdate><title>Single-copy transgenic wheat generated through the resolution of complex integration patterns</title><author>Srivastava, V ; Anderson, O.D ; Ow, D.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-315dcc6e1521c5d64ce70d5110e8ca053e740650c0fdec4553a4b36123cced3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>alleles</topic><topic>bar gene</topic><topic>bilanafos</topic><topic>biolistics</topic><topic>Biological Sciences</topic><topic>Chimera</topic><topic>Chromosome Inversion</topic><topic>cre locus</topic><topic>cre recombinase</topic><topic>DNA</topic><topic>DNA probes</topic><topic>Embryos</topic><topic>Gene Dosage</topic><topic>gene transfer</topic><topic>genes</topic><topic>Genetic loci</topic><topic>genetic recombination</topic><topic>genetic transformation</topic><topic>Genetic Vectors</topic><topic>Genomics</topic><topic>herbicide resistance</topic><topic>loci</topic><topic>lox allele</topic><topic>nucleases</topic><topic>Nucleic acid hybridization</topic><topic>Plants</topic><topic>Plants, Genetically Modified</topic><topic>plasmid vectors</topic><topic>Recombination, Genetic</topic><topic>reporter genes</topic><topic>segregation</topic><topic>single-copy transgenes</topic><topic>site-specific recombination</topic><topic>Transformation, Genetic</topic><topic>Transgenes</topic><topic>Transgenic plants</topic><topic>Triticum - genetics</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Srivastava, V</creatorcontrib><creatorcontrib>Anderson, O.D</creatorcontrib><creatorcontrib>Ow, D.W</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Srivastava, V</au><au>Anderson, O.D</au><au>Ow, D.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-copy transgenic wheat generated through the resolution of complex integration patterns</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1999-09-28</date><risdate>1999</risdate><volume>96</volume><issue>20</issue><spage>11117</spage><epage>11121</epage><pages>11117-11121</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flanked by recombination sites in an inverted orientation. Regardless of the number of copies integrated between the outermost transgenes, recombination between the outermost sites resolves the integrated molecules into a single copy. An example of this strategy has been demonstrated with wheat transformation, where four of four multiple-copy loci were resolved successfully into single-copy transgenes.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>10500139</pmid><doi>10.1073/pnas.96.20.11117</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1999-09, Vol.96 (20), p.11117-11121 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | alleles bar gene bilanafos biolistics Biological Sciences Chimera Chromosome Inversion cre locus cre recombinase DNA DNA probes Embryos Gene Dosage gene transfer genes Genetic loci genetic recombination genetic transformation Genetic Vectors Genomics herbicide resistance loci lox allele nucleases Nucleic acid hybridization Plants Plants, Genetically Modified plasmid vectors Recombination, Genetic reporter genes segregation single-copy transgenes site-specific recombination Transformation, Genetic Transgenes Transgenic plants Triticum - genetics Triticum aestivum Wheat |
| title | Single-copy transgenic wheat generated through the resolution of complex integration patterns |
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