An Inverse Relationship between T Cell Receptor Affinity and Antigen Dose during CD4+ T Cell Responses in vivo and in vitro
Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expande...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1999-08, Vol.96 (17), p.9781-9786 |
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| container_end_page | 9786 |
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| container_issue | 17 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 96 |
| creator | Rees, William Bender, Jeremy Teague, T. Kent Kedl, Ross M. Crawford, Frances Marrack, Philippa Kappler, John |
| description | Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on secondary immunization. Equating the extent of binding of this reagent to T cell receptor affinity, we saw little correlation of immunizing peptide dose to T cell receptor affinity at the peak of the primary response. However, there was an inverse relation between peptide dose and the apparent receptor affinity of the T cells that were present several months after a primary response or after a secondary stimulation either in vivo or in vitro. |
| doi_str_mv | 10.1073/pnas.96.17.9781 |
| format | Article |
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Kent ; Kedl, Ross M. ; Crawford, Frances ; Marrack, Philippa ; Kappler, John</creator><creatorcontrib>Rees, William ; Bender, Jeremy ; Teague, T. Kent ; Kedl, Ross M. ; Crawford, Frances ; Marrack, Philippa ; Kappler, John</creatorcontrib><description>Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on secondary immunization. Equating the extent of binding of this reagent to T cell receptor affinity, we saw little correlation of immunizing peptide dose to T cell receptor affinity at the peak of the primary response. 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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antigens Biological Sciences CD4-Positive T-Lymphocytes - immunology Cultured cells Dosage Flow Cytometry Histocompatibility Antigens Class II - immunology Histograms Hyaluronan Receptors - immunology Hybridomas Hybridomas - immunology Immunization Immunization, Secondary Immunology Leukocytes Mice Mice, Inbred C57BL Molecules Reagents Receptors Receptors, Antigen, T-Cell - physiology T lymphocytes |
| title | An Inverse Relationship between T Cell Receptor Affinity and Antigen Dose during CD4+ T Cell Responses in vivo and in vitro |
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