Proteolytic Processing of the Aplysia Egg-Laying Hormone Prohormone

By using matrix-assisted laser desorption/ionization time-of-flight MS, individual peptidergic neurons from Aplysia are assayed. A semiquantitative method is developed for comparing single-cell profiles by using spectral normalization, and peptides are localized to specific cells by mass spectrometr...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1998-03, Vol.95 (7), p.3972-3977
Hauptverfasser:	Garden, Rebecca W., Shippy, Scott A., Li, Lingjun, Moroz, Tatiana P., Sweedler, Jonathan V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Abdominal ganglia Animals Aplysia Biological Sciences Brain chemistry Calcium - metabolism Cells Hormones Hydrolysis Invertebrate Hormones - analysis Invertebrate Hormones - metabolism Lasers Mass spectra Mass Spectrometry Mollusks Neural stem cells Neurology Neurons Neurons - metabolism Peptides Physical Sciences Salts Stem cells
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