Ribosome Display Efficiently Selects and Evolves High-Affinity Antibodies in vitro from Immune Libraries

Ribosome display was applied for affinity selection of antibody single-chain fragments (scFv) from a diverse library generated from mice immunized with a variant peptide of the transcription factor GCN4 dimerization domain. After three rounds of ribosome display, positive scFvs were isolated and cha...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1998-11, Vol.95 (24), p.14130-14135
Hauptverfasser:	Hanes, Jozef, Jermutus, Lutz, Weber-Bornhauser, Susanne, Bosshard, Hans Rudolf, Plückthun, Andreas
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Animals Antibodies Antibody Affinity Antigens Biochemistry Biological Sciences Chromatography, Affinity Consensus sequence Enzyme-Linked Immunosorbent Assay Gels Immunoglobulin Fragments - chemistry Immunoglobulin Fragments - genetics Immunoglobulin Fragments - metabolism Immunoglobulin Heavy Chains - chemistry Immunoglobulin Heavy Chains - genetics Immunoglobulin Heavy Chains - metabolism Immunoglobulin Light Chains - chemistry Immunoglobulin Light Chains - genetics Immunoglobulin Light Chains - metabolism Immunoglobulin Variable Region - chemistry Immunoglobulin Variable Region - genetics Immunoglobulin Variable Region - metabolism Kinetics Libraries Messenger RNA Mice Molecular Sequence Data Peptide Library Polymerase chain reaction Proteins Recombinant Proteins - chemistry Recombinant Proteins - metabolism Ribosomes Ribosomes - metabolism RNA RNA, Messenger - genetics Rodents Sequence Alignment Sequence Homology, Amino Acid Solutions Spleen - immunology Transcription, Genetic Yeast
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