Directed Disruption of the Tobacco NdhB Gene Impairs Cyclic Electron Flow around Photosystem I
To evaluate the physiological significance of cyclic electron flow around photosystem (PS) I, we used a reverse genetic approach to focus on 11 chloroplast genes that encode homologs of mitochondrial complex I subunits (ndhA-K). Since their discovery, the exact function of the respiratory components...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1998-08, Vol.95 (16), p.9705-9709 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Shikanai, Toshiharu Endo, Tsuyoshi Hashimoto, Takashi Yamada, Yasuyuki Asada, Kozi Yokota, Akiho |
| description | To evaluate the physiological significance of cyclic electron flow around photosystem (PS) I, we used a reverse genetic approach to focus on 11 chloroplast genes that encode homologs of mitochondrial complex I subunits (ndhA-K). Since their discovery, the exact function of the respiratory components in plant chloroplasts has been a matter of discussion. We disrupted one of these genes (ndhB) in tobacco by chloroplast transformation. Analysis of the transient increase in chlorophyll fluorescence after actinic light illumination and the redox kinetics of P700 (reaction center chlorophylls of PS I) suggest that the cyclic electron flow around PS I is impaired in the ndhB-deficient transformants. Transformants grew normally in a greenhouse, suggesting that the cyclic electron flow around PS I mediated by ndh gene products is dispensable in tobacco under mild environmental conditions. |
| doi_str_mv | 10.1073/pnas.95.16.9705 |
| format | Article |
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Since their discovery, the exact function of the respiratory components in plant chloroplasts has been a matter of discussion. We disrupted one of these genes (ndhB) in tobacco by chloroplast transformation. Analysis of the transient increase in chlorophyll fluorescence after actinic light illumination and the redox kinetics of P700 (reaction center chlorophylls of PS I) suggest that the cyclic electron flow around PS I is impaired in the ndhB-deficient transformants. 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Transformants grew normally in a greenhouse, suggesting that the cyclic electron flow around PS I mediated by ndh gene products is dispensable in tobacco under mild environmental conditions.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Base Sequence</subject><subject>Biological Sciences</subject><subject>Chlorophyll - metabolism</subject><subject>Chlorophylls</subject><subject>Chloroplasts</subject><subject>Cyanobacteria</subject><subject>Dehydrogenases</subject><subject>DNA Primers</subject><subject>Electrons</subject><subject>Fluorescence</subject><subject>Genes</subject><subject>Genes, Plant</subject><subject>Genetics</subject><subject>Kinetics</subject><subject>Leaves</subject><subject>Light-Harvesting Protein Complexes</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Photosynthesis</subject><subject>Photosynthetic Reaction Center Complex Proteins - metabolism</subject><subject>Photosystem I Protein Complex</subject><subject>Plant Proteins - genetics</subject><subject>Plants</subject><subject>Plants, Toxic</subject><subject>Tobacco</subject><subject>Transformation, Genetic</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1r3DAQhkVpSbdpz4VCisglJ29GlmVZ0Eu7-ehCSHtIrxXWh7NevJYjyU3330fLLia55DSH93mGYV6EPhOYE-D0fOjrMBdsTsq54MDeoBkBQbKyEPAWzQBynlVFXrxHH0JYA4BgFRyhI1FWghRshv5etN7qaA2-aIMfh9i6HrsGx5XFd07VWjt8a1Y_8LXtLV5uhrr1AS-2ums1vuyS6pNw1blHXHs39gb_XrnowjZEu8HLj-hdU3fBfjrMY_Tn6vJu8TO7-XW9XHy_yXRR8ZhVNi8EVxXXZdmAUayyUAnKDGU5YcRQo_KSgKKGC6Gs4cxYaww0lCqjFNBj9G2_dxjVxhpt--jrTg6-3dR-K13dypdJ367kvfsnc1IATfrpQffuYbQhyrUbfZ8uljkQytM38wSd7yHtXQjeNtN6AnJXhtyVIQWTpJS7MpJx8vyqiT98P-Vnh3wnTum0QDZj10X7Pyby66tkAr7sgXWIzk9EwRgV9AmWZKgY</recordid><startdate>19980804</startdate><enddate>19980804</enddate><creator>Shikanai, Toshiharu</creator><creator>Endo, Tsuyoshi</creator><creator>Hashimoto, Takashi</creator><creator>Yamada, Yasuyuki</creator><creator>Asada, Kozi</creator><creator>Yokota, Akiho</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19980804</creationdate><title>Directed Disruption of the Tobacco NdhB Gene Impairs Cyclic Electron Flow around Photosystem I</title><author>Shikanai, Toshiharu ; Endo, Tsuyoshi ; Hashimoto, Takashi ; Yamada, Yasuyuki ; Asada, Kozi ; Yokota, Akiho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-8e2497b87c66f0db58e08935d352151d3db2610b3d799bed75deedd0f33bdbb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Base Sequence</topic><topic>Biological Sciences</topic><topic>Chlorophyll - metabolism</topic><topic>Chlorophylls</topic><topic>Chloroplasts</topic><topic>Cyanobacteria</topic><topic>Dehydrogenases</topic><topic>DNA Primers</topic><topic>Electrons</topic><topic>Fluorescence</topic><topic>Genes</topic><topic>Genes, Plant</topic><topic>Genetics</topic><topic>Kinetics</topic><topic>Leaves</topic><topic>Light-Harvesting Protein Complexes</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Photosynthesis</topic><topic>Photosynthetic Reaction Center Complex Proteins - metabolism</topic><topic>Photosystem I Protein Complex</topic><topic>Plant Proteins - genetics</topic><topic>Plants</topic><topic>Plants, Toxic</topic><topic>Tobacco</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shikanai, Toshiharu</creatorcontrib><creatorcontrib>Endo, Tsuyoshi</creatorcontrib><creatorcontrib>Hashimoto, Takashi</creatorcontrib><creatorcontrib>Yamada, Yasuyuki</creatorcontrib><creatorcontrib>Asada, Kozi</creatorcontrib><creatorcontrib>Yokota, Akiho</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shikanai, Toshiharu</au><au>Endo, Tsuyoshi</au><au>Hashimoto, Takashi</au><au>Yamada, Yasuyuki</au><au>Asada, Kozi</au><au>Yokota, Akiho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Directed Disruption of the Tobacco NdhB Gene Impairs Cyclic Electron Flow around Photosystem I</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1998-08-04</date><risdate>1998</risdate><volume>95</volume><issue>16</issue><spage>9705</spage><epage>9709</epage><pages>9705-9709</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>To evaluate the physiological significance of cyclic electron flow around photosystem (PS) I, we used a reverse genetic approach to focus on 11 chloroplast genes that encode homologs of mitochondrial complex I subunits (ndhA-K). 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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1998-08, Vol.95 (16), p.9705-9709 |
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| subjects | Adenosine Triphosphate - metabolism Base Sequence Biological Sciences Chlorophyll - metabolism Chlorophylls Chloroplasts Cyanobacteria Dehydrogenases DNA Primers Electrons Fluorescence Genes Genes, Plant Genetics Kinetics Leaves Light-Harvesting Protein Complexes Nicotiana - genetics Nicotiana - metabolism Oxidation-Reduction Photosynthesis Photosynthetic Reaction Center Complex Proteins - metabolism Photosystem I Protein Complex Plant Proteins - genetics Plants Plants, Toxic Tobacco Transformation, Genetic |
| title | Directed Disruption of the Tobacco NdhB Gene Impairs Cyclic Electron Flow around Photosystem I |
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