Receptor-Mediated Activation of a Plant Ca2+-Permeable Ion Channel Involved in Pathogen Defense
Pathogen recognition at the plant cell surface typically results in the initiation of a multicomponent defense response. Transient influx of Ca2+across the plasma membrane is postulated to be part of the signaling chain leading to pathogen resistance. Patch-clamp analysis of parsley protoplasts reve...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1997-03, Vol.94 (6), p.2751-2755 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Zimmermann, Sabine Nurnberger, Thorsten Frachisse, Jean-Marie Wirtz, Wolfgang Guern, Jean Hedrich, Rainer Scheel, Dierk |
| description | Pathogen recognition at the plant cell surface typically results in the initiation of a multicomponent defense response. Transient influx of Ca2+across the plasma membrane is postulated to be part of the signaling chain leading to pathogen resistance. Patch-clamp analysis of parsley protoplasts revealed a novel Ca2+-permeable, La3+-sensitive plasma membrane ion channel of large conductance (309 pS in 240 mM CaCl2). At an extracellular Ca2+concentration of 1 mM, which is representative of the plant cell apoplast, unitary channel conductance was determined to be 80 pS. This ion channel (LEAC, for large conductance elicitor-aactivated ion channel) is reversibly activated upon treatment of parsley protoplasts with an oligopeptide elicitor derived from a cell wall protein of Phytophthora sojae. Structural features of the elicitor found previously to be essential for receptor binding, induction of defense-related gene expression, and phytoalexin formation are identical to those required for activation of LEAC. Thus, receptor-mediated stimulation of this channel appears to be causally involved in the signaling cascade triggering pathogen defense in parsley. |
| doi_str_mv | 10.1073/pnas.94.6.2751 |
| format | Article |
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Transient influx of Ca2+across the plasma membrane is postulated to be part of the signaling chain leading to pathogen resistance. Patch-clamp analysis of parsley protoplasts revealed a novel Ca2+-permeable, La3+-sensitive plasma membrane ion channel of large conductance (309 pS in 240 mM CaCl2). At an extracellular Ca2+concentration of 1 mM, which is representative of the plant cell apoplast, unitary channel conductance was determined to be 80 pS. This ion channel (LEAC, for large conductance elicitor-aactivated ion channel) is reversibly activated upon treatment of parsley protoplasts with an oligopeptide elicitor derived from a cell wall protein of Phytophthora sojae. Structural features of the elicitor found previously to be essential for receptor binding, induction of defense-related gene expression, and phytoalexin formation are identical to those required for activation of LEAC. Thus, receptor-mediated stimulation of this channel appears to be causally involved in the signaling cascade triggering pathogen defense in parsley.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.94.6.2751</identifier><identifier>PMID: 11038609</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Biological Sciences ; Botany ; Cell culture techniques ; Cell membranes ; Cell walls ; Cells ; Flowers & plants ; Ion channels ; Molecular biology ; Parsley ; Pathogens ; Plant cells ; Plants ; Protoplasts ; Receptors</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1997-03, Vol.94 (6), p.2751-2755</ispartof><rights>Copyright 1997 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Mar 18, 1997</rights><rights>Copyright © 1997, The National Academy of Sciences of the USA 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/94/6.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/41712$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/41712$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11038609$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zimmermann, Sabine</creatorcontrib><creatorcontrib>Nurnberger, Thorsten</creatorcontrib><creatorcontrib>Frachisse, Jean-Marie</creatorcontrib><creatorcontrib>Wirtz, Wolfgang</creatorcontrib><creatorcontrib>Guern, Jean</creatorcontrib><creatorcontrib>Hedrich, Rainer</creatorcontrib><creatorcontrib>Scheel, Dierk</creatorcontrib><title>Receptor-Mediated Activation of a Plant Ca2+-Permeable Ion Channel Involved in Pathogen Defense</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Pathogen recognition at the plant cell surface typically results in the initiation of a multicomponent defense response. Transient influx of Ca2+across the plasma membrane is postulated to be part of the signaling chain leading to pathogen resistance. Patch-clamp analysis of parsley protoplasts revealed a novel Ca2+-permeable, La3+-sensitive plasma membrane ion channel of large conductance (309 pS in 240 mM CaCl2). At an extracellular Ca2+concentration of 1 mM, which is representative of the plant cell apoplast, unitary channel conductance was determined to be 80 pS. This ion channel (LEAC, for large conductance elicitor-aactivated ion channel) is reversibly activated upon treatment of parsley protoplasts with an oligopeptide elicitor derived from a cell wall protein of Phytophthora sojae. Structural features of the elicitor found previously to be essential for receptor binding, induction of defense-related gene expression, and phytoalexin formation are identical to those required for activation of LEAC. Thus, receptor-mediated stimulation of this channel appears to be causally involved in the signaling cascade triggering pathogen defense in parsley.</description><subject>Biological Sciences</subject><subject>Botany</subject><subject>Cell culture techniques</subject><subject>Cell membranes</subject><subject>Cell walls</subject><subject>Cells</subject><subject>Flowers & plants</subject><subject>Ion channels</subject><subject>Molecular biology</subject><subject>Parsley</subject><subject>Pathogens</subject><subject>Plant cells</subject><subject>Plants</subject><subject>Protoplasts</subject><subject>Receptors</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNp9kUuP0zAUhS0EYsrAlgUSKGLBBqVcPxNLbEblVWkQFYK15Tg301SuXWKngn-PRzNUAwtWd3G-c1-HkKcUlhQa_uYQbFpqsVRL1kh6jywoaForoeE-WQCwpm4FE2fkUUo7ANCyhYfkjFLgrQK9IOYrOjzkONWfsR9txr66cHk82jzGUMWhstXG25CrlWWv6w1Oe7Sdx2pd1NXWhoC-Wodj9MfiHEO1sXkbrzBU73DAkPAxeTBYn_DJbT0n3z-8_7b6VF9--bheXVzWO6ZprgU2XNAWNAeLomfKdYAWO9W1XPUMRUMF0Fbaoe9cD612QvJOOwfIO6mBn5O3N30Pc7fH3mHIk_XmMI17O_0y0Y7mbyWMW3MVj4YBVazYX93ap_hjxpTNfkwOfTkd45xMo5UE3coCvvwH3MV5CuW06068kbJpC_Ti7jKnLf68_c64Et9J1sIocx2jGWbvM_7MBXz-P7Doz270XSoZngBBG8r4b8XJpPk</recordid><startdate>19970318</startdate><enddate>19970318</enddate><creator>Zimmermann, Sabine</creator><creator>Nurnberger, Thorsten</creator><creator>Frachisse, Jean-Marie</creator><creator>Wirtz, Wolfgang</creator><creator>Guern, Jean</creator><creator>Hedrich, Rainer</creator><creator>Scheel, Dierk</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><general>The National Academy of Sciences of the USA</general><scope>NPM</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970318</creationdate><title>Receptor-Mediated Activation of a Plant Ca2+-Permeable Ion Channel Involved in Pathogen Defense</title><author>Zimmermann, Sabine ; 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| source | Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Biological Sciences Botany Cell culture techniques Cell membranes Cell walls Cells Flowers & plants Ion channels Molecular biology Parsley Pathogens Plant cells Plants Protoplasts Receptors |
| title | Receptor-Mediated Activation of a Plant Ca2+-Permeable Ion Channel Involved in Pathogen Defense |
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