Identification and Characterization of Saccharomyces cerevisiae EXO1, a Gene Encoding an Exonuclease that Interacts with MSH2
A two-hybrid screen was used to identify Saccharomyces cerevisiae genes encoding proteins that interact with MSH2. One gene was found to encode a homologue of Schizosaccharomyces pombe EXO1, a double-stranded DNA-specific 5′-3′exonuclease. S. cerevisiae EXO1 interacted with both S. cerevisiae and hu...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1997-07, Vol.94 (14), p.7487-7492 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Tishkoff, Daniel X. Boerger, Adrienne L. Bertrand, Pascale Filosi, Nicole Gaida, Gretchen M. Kane, Michael F. Kolodner, Richard D. |
| description | A two-hybrid screen was used to identify Saccharomyces cerevisiae genes encoding proteins that interact with MSH2. One gene was found to encode a homologue of Schizosaccharomyces pombe EXO1, a double-stranded DNA-specific 5′-3′exonuclease. S. cerevisiae EXO1 interacted with both S. cerevisiae and human MSH2 in two-hybrid and coimmunoprecipitation experiments. exo1 mutants showed a mutator phenotype, and epistasis analysis was consistent with EXO1 functioning in the MSH2-dependent mismatch repair pathway. exo1 mutations were lethal in combination with rad27 mutations, and overexpression of EXO1 suppressed both the temperature sensitive and mutator phenotypes of rad27 mutants. |
| doi_str_mv | 10.1073/pnas.94.14.7487 |
| format | Article |
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One gene was found to encode a homologue of Schizosaccharomyces pombe EXO1, a double-stranded DNA-specific 5′-3′exonuclease. S. cerevisiae EXO1 interacted with both S. cerevisiae and human MSH2 in two-hybrid and coimmunoprecipitation experiments. exo1 mutants showed a mutator phenotype, and epistasis analysis was consistent with EXO1 functioning in the MSH2-dependent mismatch repair pathway. exo1 mutations were lethal in combination with rad27 mutations, and overexpression of EXO1 suppressed both the temperature sensitive and mutator phenotypes of rad27 mutants.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.94.14.7487</identifier><identifier>PMID: 9207118</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Antibodies ; Biological Sciences ; DNA ; DNA Repair Enzymes ; DNA-Binding Proteins - genetics ; Exodeoxyribonucleases - genetics ; Exonucleases - genetics ; Fungal Proteins ; Genes ; Genes, Fungal ; Genetic mutation ; Genetics ; Genomics ; Human genetics ; Humans ; Molecular Sequence Data ; MutS Homolog 2 Protein ; Open reading frames ; Phenotypes ; Plasmids ; Polymerase chain reaction ; Proteins ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins ; Schizosaccharomyces pombe ; Schizosaccharomyces pombe Proteins ; Sequence Homology, Amino Acid ; Yeast</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1997-07, Vol.94 (14), p.7487-7492</ispartof><rights>Copyright 1997 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Jul 8, 1997</rights><rights>Copyright © 1997, The National Academy of Sciences of the USA 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-1c045adac2ad74b9d5d3566af797efadea026537caff7a58176d8c7de332a7a43</citedby><cites>FETCH-LOGICAL-c518t-1c045adac2ad74b9d5d3566af797efadea026537caff7a58176d8c7de332a7a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/94/14.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/42705$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/42705$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9207118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tishkoff, Daniel X.</creatorcontrib><creatorcontrib>Boerger, Adrienne L.</creatorcontrib><creatorcontrib>Bertrand, Pascale</creatorcontrib><creatorcontrib>Filosi, Nicole</creatorcontrib><creatorcontrib>Gaida, Gretchen M.</creatorcontrib><creatorcontrib>Kane, Michael F.</creatorcontrib><creatorcontrib>Kolodner, Richard D.</creatorcontrib><title>Identification and Characterization of Saccharomyces cerevisiae EXO1, a Gene Encoding an Exonuclease that Interacts with MSH2</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A two-hybrid screen was used to identify Saccharomyces cerevisiae genes encoding proteins that interact with MSH2. 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| issn | 0027-8424 1091-6490 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Antibodies Biological Sciences DNA DNA Repair Enzymes DNA-Binding Proteins - genetics Exodeoxyribonucleases - genetics Exonucleases - genetics Fungal Proteins Genes Genes, Fungal Genetic mutation Genetics Genomics Human genetics Humans Molecular Sequence Data MutS Homolog 2 Protein Open reading frames Phenotypes Plasmids Polymerase chain reaction Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid Yeast |
| title | Identification and Characterization of Saccharomyces cerevisiae EXO1, a Gene Encoding an Exonuclease that Interacts with MSH2 |
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