A Mammalian Adaptor Protein with Conserved Src Homology 2 and Phosphotyrosine-Binding Domains is Related to Shc and is Specifically Expressed in the Brain

The Shc adaptor protein, hereafter referred to as ShcA, possesses two distinct phosphotyrosine-recognition modules, a C-terminal Src homology 2 (SH2) domain and an N-terminal phosphotyrosine-binding (PTB) domain, and is itself phosphorylated on tyrosine in response to many extracellular signals. Pho...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1996-04, Vol.93 (7), p.2729-2734
Hauptverfasser: O'Bryan, John P., Songyang, Zhou, Cantley, Lewis, Der, Channing J., Pawson, Tony
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container_end_page 2734
container_issue 7
container_start_page 2729
container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 93
creator O'Bryan, John P.
Songyang, Zhou
Cantley, Lewis
Der, Channing J.
Pawson, Tony
description The Shc adaptor protein, hereafter referred to as ShcA, possesses two distinct phosphotyrosine-recognition modules, a C-terminal Src homology 2 (SH2) domain and an N-terminal phosphotyrosine-binding (PTB) domain, and is itself phosphorylated on tyrosine in response to many extracellular signals. Phosphorylation of human ShcA at Tyr-317 within its central (CH1) region induces binding to the Grb2 SH2 domain and is thereby implicated in activation of the Ras pathway. Two shc-related genes (shcB and shcC) have been identified in the mouse. shcB is closely related to human SCK, while shcC has not yet been found in other organisms. The ShcC protein is predicted to have a C-terminal SH2 domain, a CH1 region with a putative Grb2-binding site, and an N-terminal PTB domain. The ShcC and ShcB SH2 domains bind phosphotyrosine-containing peptides and receptors with a specificity related to, but distinct from, that of the ShcA SH2 domain. The ShcC PTB domain specifically associates in vitro with the autophosphorylated receptors for nerve growth factor and epidermal growth factor. These results indicate that ShcC has functional SH2 and PTB domains. In contrast to shcA, which is widely expressed, shcC RNA and proteins are predominantly expressed in the adult brain. These results suggest that ShcC may mediate signaling from tyrosine kinases in the nervous system, such as receptors for neurotrophins.
doi_str_mv 10.1073/pnas.93.7.2729
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identifier ISSN: 0027-8424
ispartof Proceedings of the National Academy of Sciences - PNAS, 1996-04, Vol.93 (7), p.2729-2734
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source MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry
subjects 3T3 Cells
Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Amino acids
Animals
Antibodies
Base Sequence
Binding Sites
Biochemistry
Brain
Brain - metabolism
Cell Line
Cloning, Molecular
Complementary DNA
Conserved Sequence
DNA Primers
Drosophila
Drosophila - metabolism
ErbB Receptors - metabolism
Female
Humans
Mammals
Mice
Molecular Sequence Data
Multigene Family
Nerve Tissue Proteins - chemistry
Neuropeptides
Organ Specificity
Phosphorylation
Phosphotyrosine
Polymerase Chain Reaction
Protein Biosynthesis
Proteins
Proteins - chemistry
Proteins - metabolism
Receptors
Receptors, Nerve Growth Factor - metabolism
Recombinant Proteins - biosynthesis
Recombinant Proteins - metabolism
RNA
Sequence Homology, Amino Acid
Shc Signaling Adaptor Proteins
Src Homology 2 Domain-Containing, Transforming Protein 2
Src Homology 2 Domain-Containing, Transforming Protein 3
src Homology Domains
title A Mammalian Adaptor Protein with Conserved Src Homology 2 and Phosphotyrosine-Binding Domains is Related to Shc and is Specifically Expressed in the Brain
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