Visualization of the Thyrotropin-Releasing Hormone Receptor and Its Ligand During Endocytosis and Recycling

Endocytosis and recycling of both thyrotropin-releasing hormone (TRH) and its G-protein-coupled receptor were visualized by conventional and confocal fluorescence microscopy in pituitary cells using a rhodamine-labeled TRH analog (Rhod-TRH) and indirect immunofluorescent staining of cells stably tra...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1995-01, Vol.92 (2), p.512-516
Hauptverfasser:	Ashworth, Rachel, Yu, Run, Nelson, Eric J., Dermer, Shari, Gershengorn, Marvin C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Biological Transport Cell lines Cell membranes Cellular immunity Endocytosis Endocytosis - physiology Epitopes Fluorescence Immunohistochemistry Internalization Ligands Microscopy, Fluorescence Molecular Sequence Data Pituitary Gland - cytology Pituitary Gland - metabolism Receptors Receptors, Thyrotropin-Releasing Hormone - genetics Receptors, Thyrotropin-Releasing Hormone - metabolism Recombinant Proteins - metabolism Recycling Rhodamines Rodentia Thyrotropin-Releasing Hormone - metabolism
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