Accelerated evolution in the protein-coding regions is universal in crotalinae snake venom gland phospholipase A2 isozyme genes
The nucleotide sequences of four genes encoding Trimeresurus gramineus (green habu snake, crotalinae) venom gland phospholipase A2 (PLA2; phosphatidylcholine 2-acylhydrolase, EC 3.1.1.4) isozymes were compared internally and externally with those of six genes encoding Trimeresurus flavoviridis (habu...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1995-06, Vol.92 (12), p.5605-5609 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5609 |
|---|---|
| container_issue | 12 |
| container_start_page | 5605 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 92 |
| creator | Nakashima, K Nobuhisa, I Deshimaru, M Nakai, M Ogawa, T Shimohigashi, Y Fukumaki, Y Hattori, M Sakaki, Y Hattori, S |
| description | The nucleotide sequences of four genes encoding Trimeresurus gramineus (green habu snake, crotalinae) venom gland phospholipase A2 (PLA2; phosphatidylcholine 2-acylhydrolase, EC 3.1.1.4) isozymes were compared internally and externally with those of six genes encoding Trimeresurus flavoviridis (habu snake, crotalinae) venom gland PLA2 isozymes. The numbers of nucleotide substitutions per site (KN) for the noncoding regions including introns were one-third to one-eighth of the numbers of nucleotide substitutions per synonymous site (KS) for the protein-coding regions of exons, indicating that the noncoding regions are much more conserved than the protein-coding regions. The KN values for the introns were found to be nearly equivalent to those of introns of T. gramineus and T. flavoviridis TATA box-binding protein genes, which are assumed to be a general (nonvenomous) gene. Thus, it is evident that the introns of venom gland PLA2 isozyme genes have evolved at a similar rate to those of nonvenomous genes. The numbers of nucleotide substitutions per nonsynonymous site (KA) were close to or larger than the KS values for the protein-coding regions in venom gland PLA2 isozyme genes. All of the data combined reveal that Darwinian-type accelerated evolution has universally occurred only in the protein-coding regions of crotalinae snake venom PLA2 isozyme genes. |
| doi_str_mv | 10.1073/pnas.92.12.5605 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pnas_</sourceid><recordid>TN_cdi_pnas_primary_92_12_5605</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>6794957</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4495-b97a1470ddd57492dd3e6923483e6812a1d430fad2824bfb87612b4ab55a1aab3</originalsourceid><addsrcrecordid>eNp9kc1v1DAQxS0EKkvhzAlkcYBTtrZjx7HEZVXxJVXiAmfLiSdZF68d7GRFufCv46hLRTlgyZrD-73RzDyEnlOypUTWF1MweavYlrKtaIh4gDaUKFo1XJGHaEMIk1XLGX-MnuR8TQhRoiVn6EyWJ0SzQb92fQ8ekpnBYjhGv8wuBuwCnveApxRncKHqo3VhxAnGImbsMl6CO0LKxq9oXzDjXTCAczDfAB8hxAMevQkWT_uYy_duMhnwjhV3_HlzADxCgPwUPRqMz_DsVM_R1_fvvlx-rK4-f_h0ubuqes6VqDolDeWSWGuF5IpZW0OjWM3bUlvKDLW8JoOxrGW8G7pWNpR13HRCGGpMV5-jt7d9p6U7gO0hzMl4PSV3MOlGR-P0fSW4vR7jUXMquSj21yd7it8XyLM-uFwOVzaEuGQtZU0b1azgq3_A67ikUFbTjFBWEKoKdHELlcPlnGC4m4MSvcaq11i1YpoyvcZaHC_-Hv-OP-VY9JcnfTX-Ue81ePNfQA-L9zP8mOvfXZe5lQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201296519</pqid></control><display><type>article</type><title>Accelerated evolution in the protein-coding regions is universal in crotalinae snake venom gland phospholipase A2 isozyme genes</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Nakashima, K ; Nobuhisa, I ; Deshimaru, M ; Nakai, M ; Ogawa, T ; Shimohigashi, Y ; Fukumaki, Y ; Hattori, M ; Sakaki, Y ; Hattori, S</creator><creatorcontrib>Nakashima, K ; Nobuhisa, I ; Deshimaru, M ; Nakai, M ; Ogawa, T ; Shimohigashi, Y ; Fukumaki, Y ; Hattori, M ; Sakaki, Y ; Hattori, S</creatorcontrib><description>The nucleotide sequences of four genes encoding Trimeresurus gramineus (green habu snake, crotalinae) venom gland phospholipase A2 (PLA2; phosphatidylcholine 2-acylhydrolase, EC 3.1.1.4) isozymes were compared internally and externally with those of six genes encoding Trimeresurus flavoviridis (habu snake, crotalinae) venom gland PLA2 isozymes. The numbers of nucleotide substitutions per site (KN) for the noncoding regions including introns were one-third to one-eighth of the numbers of nucleotide substitutions per synonymous site (KS) for the protein-coding regions of exons, indicating that the noncoding regions are much more conserved than the protein-coding regions. The KN values for the introns were found to be nearly equivalent to those of introns of T. gramineus and T. flavoviridis TATA box-binding protein genes, which are assumed to be a general (nonvenomous) gene. Thus, it is evident that the introns of venom gland PLA2 isozyme genes have evolved at a similar rate to those of nonvenomous genes. The numbers of nucleotide substitutions per nonsynonymous site (KA) were close to or larger than the KS values for the protein-coding regions in venom gland PLA2 isozyme genes. All of the data combined reveal that Darwinian-type accelerated evolution has universally occurred only in the protein-coding regions of crotalinae snake venom PLA2 isozyme genes.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.92.12.5605</identifier><identifier>PMID: 7777556</identifier><language>eng</language><publisher>United States: National Acad Sciences</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological Evolution ; Crotalid Venoms - enzymology ; DNA, Complementary ; Evolution ; Exons ; Genes ; Introns ; Isoenzymes - genetics ; Molecular Sequence Data ; Mutation ; Nucleotides - genetics ; Phospholipases A - chemistry ; Phospholipases A - genetics ; Phospholipases A2 ; Proteins ; Snakes</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1995-06, Vol.92 (12), p.5605-5609</ispartof><rights>Copyright National Academy of Sciences Jun 6, 1995</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4495-b97a1470ddd57492dd3e6923483e6812a1d430fad2824bfb87612b4ab55a1aab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/92/12.cover.gif</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC41745/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC41745/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7777556$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakashima, K</creatorcontrib><creatorcontrib>Nobuhisa, I</creatorcontrib><creatorcontrib>Deshimaru, M</creatorcontrib><creatorcontrib>Nakai, M</creatorcontrib><creatorcontrib>Ogawa, T</creatorcontrib><creatorcontrib>Shimohigashi, Y</creatorcontrib><creatorcontrib>Fukumaki, Y</creatorcontrib><creatorcontrib>Hattori, M</creatorcontrib><creatorcontrib>Sakaki, Y</creatorcontrib><creatorcontrib>Hattori, S</creatorcontrib><title>Accelerated evolution in the protein-coding regions is universal in crotalinae snake venom gland phospholipase A2 isozyme genes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The nucleotide sequences of four genes encoding Trimeresurus gramineus (green habu snake, crotalinae) venom gland phospholipase A2 (PLA2; phosphatidylcholine 2-acylhydrolase, EC 3.1.1.4) isozymes were compared internally and externally with those of six genes encoding Trimeresurus flavoviridis (habu snake, crotalinae) venom gland PLA2 isozymes. The numbers of nucleotide substitutions per site (KN) for the noncoding regions including introns were one-third to one-eighth of the numbers of nucleotide substitutions per synonymous site (KS) for the protein-coding regions of exons, indicating that the noncoding regions are much more conserved than the protein-coding regions. The KN values for the introns were found to be nearly equivalent to those of introns of T. gramineus and T. flavoviridis TATA box-binding protein genes, which are assumed to be a general (nonvenomous) gene. Thus, it is evident that the introns of venom gland PLA2 isozyme genes have evolved at a similar rate to those of nonvenomous genes. The numbers of nucleotide substitutions per nonsynonymous site (KA) were close to or larger than the KS values for the protein-coding regions in venom gland PLA2 isozyme genes. All of the data combined reveal that Darwinian-type accelerated evolution has universally occurred only in the protein-coding regions of crotalinae snake venom PLA2 isozyme genes.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological Evolution</subject><subject>Crotalid Venoms - enzymology</subject><subject>DNA, Complementary</subject><subject>Evolution</subject><subject>Exons</subject><subject>Genes</subject><subject>Introns</subject><subject>Isoenzymes - genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Nucleotides - genetics</subject><subject>Phospholipases A - chemistry</subject><subject>Phospholipases A - genetics</subject><subject>Phospholipases A2</subject><subject>Proteins</subject><subject>Snakes</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1v1DAQxS0EKkvhzAlkcYBTtrZjx7HEZVXxJVXiAmfLiSdZF68d7GRFufCv46hLRTlgyZrD-73RzDyEnlOypUTWF1MweavYlrKtaIh4gDaUKFo1XJGHaEMIk1XLGX-MnuR8TQhRoiVn6EyWJ0SzQb92fQ8ekpnBYjhGv8wuBuwCnveApxRncKHqo3VhxAnGImbsMl6CO0LKxq9oXzDjXTCAczDfAB8hxAMevQkWT_uYy_duMhnwjhV3_HlzADxCgPwUPRqMz_DsVM_R1_fvvlx-rK4-f_h0ubuqes6VqDolDeWSWGuF5IpZW0OjWM3bUlvKDLW8JoOxrGW8G7pWNpR13HRCGGpMV5-jt7d9p6U7gO0hzMl4PSV3MOlGR-P0fSW4vR7jUXMquSj21yd7it8XyLM-uFwOVzaEuGQtZU0b1azgq3_A67ikUFbTjFBWEKoKdHELlcPlnGC4m4MSvcaq11i1YpoyvcZaHC_-Hv-OP-VY9JcnfTX-Ue81ePNfQA-L9zP8mOvfXZe5lQ</recordid><startdate>19950606</startdate><enddate>19950606</enddate><creator>Nakashima, K</creator><creator>Nobuhisa, I</creator><creator>Deshimaru, M</creator><creator>Nakai, M</creator><creator>Ogawa, T</creator><creator>Shimohigashi, Y</creator><creator>Fukumaki, Y</creator><creator>Hattori, M</creator><creator>Sakaki, Y</creator><creator>Hattori, S</creator><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19950606</creationdate><title>Accelerated evolution in the protein-coding regions is universal in crotalinae snake venom gland phospholipase A2 isozyme genes</title><author>Nakashima, K ; Nobuhisa, I ; Deshimaru, M ; Nakai, M ; Ogawa, T ; Shimohigashi, Y ; Fukumaki, Y ; Hattori, M ; Sakaki, Y ; Hattori, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4495-b97a1470ddd57492dd3e6923483e6812a1d430fad2824bfb87612b4ab55a1aab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological Evolution</topic><topic>Crotalid Venoms - enzymology</topic><topic>DNA, Complementary</topic><topic>Evolution</topic><topic>Exons</topic><topic>Genes</topic><topic>Introns</topic><topic>Isoenzymes - genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Nucleotides - genetics</topic><topic>Phospholipases A - chemistry</topic><topic>Phospholipases A - genetics</topic><topic>Phospholipases A2</topic><topic>Proteins</topic><topic>Snakes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakashima, K</creatorcontrib><creatorcontrib>Nobuhisa, I</creatorcontrib><creatorcontrib>Deshimaru, M</creatorcontrib><creatorcontrib>Nakai, M</creatorcontrib><creatorcontrib>Ogawa, T</creatorcontrib><creatorcontrib>Shimohigashi, Y</creatorcontrib><creatorcontrib>Fukumaki, Y</creatorcontrib><creatorcontrib>Hattori, M</creatorcontrib><creatorcontrib>Sakaki, Y</creatorcontrib><creatorcontrib>Hattori, S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakashima, K</au><au>Nobuhisa, I</au><au>Deshimaru, M</au><au>Nakai, M</au><au>Ogawa, T</au><au>Shimohigashi, Y</au><au>Fukumaki, Y</au><au>Hattori, M</au><au>Sakaki, Y</au><au>Hattori, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accelerated evolution in the protein-coding regions is universal in crotalinae snake venom gland phospholipase A2 isozyme genes</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1995-06-06</date><risdate>1995</risdate><volume>92</volume><issue>12</issue><spage>5605</spage><epage>5609</epage><pages>5605-5609</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The nucleotide sequences of four genes encoding Trimeresurus gramineus (green habu snake, crotalinae) venom gland phospholipase A2 (PLA2; phosphatidylcholine 2-acylhydrolase, EC 3.1.1.4) isozymes were compared internally and externally with those of six genes encoding Trimeresurus flavoviridis (habu snake, crotalinae) venom gland PLA2 isozymes. The numbers of nucleotide substitutions per site (KN) for the noncoding regions including introns were one-third to one-eighth of the numbers of nucleotide substitutions per synonymous site (KS) for the protein-coding regions of exons, indicating that the noncoding regions are much more conserved than the protein-coding regions. The KN values for the introns were found to be nearly equivalent to those of introns of T. gramineus and T. flavoviridis TATA box-binding protein genes, which are assumed to be a general (nonvenomous) gene. Thus, it is evident that the introns of venom gland PLA2 isozyme genes have evolved at a similar rate to those of nonvenomous genes. The numbers of nucleotide substitutions per nonsynonymous site (KA) were close to or larger than the KS values for the protein-coding regions in venom gland PLA2 isozyme genes. All of the data combined reveal that Darwinian-type accelerated evolution has universally occurred only in the protein-coding regions of crotalinae snake venom PLA2 isozyme genes.</abstract><cop>United States</cop><pub>National Acad Sciences</pub><pmid>7777556</pmid><doi>10.1073/pnas.92.12.5605</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1995-06, Vol.92 (12), p.5605-5609 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_92_12_5605 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Base Sequence Biological Evolution Crotalid Venoms - enzymology DNA, Complementary Evolution Exons Genes Introns Isoenzymes - genetics Molecular Sequence Data Mutation Nucleotides - genetics Phospholipases A - chemistry Phospholipases A - genetics Phospholipases A2 Proteins Snakes |
| title | Accelerated evolution in the protein-coding regions is universal in crotalinae snake venom gland phospholipase A2 isozyme genes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T22%3A21%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Accelerated%20evolution%20in%20the%20protein-coding%20regions%20is%20universal%20in%20crotalinae%20snake%20venom%20gland%20phospholipase%20A2%20isozyme%20genes&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Nakashima,%20K&rft.date=1995-06-06&rft.volume=92&rft.issue=12&rft.spage=5605&rft.epage=5609&rft.pages=5605-5609&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.92.12.5605&rft_dat=%3Cproquest_pnas_%3E6794957%3C/proquest_pnas_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201296519&rft_id=info:pmid/7777556&rfr_iscdi=true |