Hypermutagenesis of RNA Using Human Immunodeficiency Virus Type 1 Reverse Transcriptase and Biased dNTP Concentrations

The finding of G → A hypermutated retroviral genomes in which up to 40% of guanines may be substituted by adenines was proposed to result from the depletion of the intracellular dCTP concentration and suggested a means to hypermutagenize nucleic acids. Using a RNA/reverse transcriptase ratio of ≈1:3...

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