Amino Acid Sequence of a Putative Sodium Channel Expressed in the Giant Axon of the Squid Loligo opalescens
A full-length cDNA encoding a putative Na+channel (GFLN1) has been cloned from a library prepared from the stellate ganglion of Loligo opalescens. The cDNA encodes a predicted protein of 1784 amino acids. Regions of the GFLN1 protein with defined functional importance (membrane span S4, the SS1 and...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1993-11, Vol.90 (21), p.10026-10030 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 10030 |
|---|---|
| container_issue | 21 |
| container_start_page | 10026 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 90 |
| creator | Joshua J. C. Rosenthal Gilly, William F. |
| description | A full-length cDNA encoding a putative Na+channel (GFLN1) has been cloned from a library prepared from the stellate ganglion of Loligo opalescens. The cDNA encodes a predicted protein of 1784 amino acids. Regions of the GFLN1 protein with defined functional importance (membrane span S4, the SS1 and SS2 segments, and interdomain III-IV) are highly conserved among all vertebrate Na+channel α-subunit structures. Northern blot hybridization and RNase protection assays verify that mRNA corresponding to GFLN1 is expressed in neurons of the giant fiber lobe that form the giant axon. We propose that GFLN1 encodes the Na+channel that has been extensively studied in the squid axon. |
| doi_str_mv | 10.1073/pnas.90.21.10026 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pnas_</sourceid><recordid>TN_cdi_pnas_primary_90_21_10026</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>2363395</jstor_id><sourcerecordid>2363395</sourcerecordid><originalsourceid>FETCH-LOGICAL-c589t-5659de8dcb1affcc7c8fa6285b9432c4125d2e984c2d9332b9fb97ecf7246b1b3</originalsourceid><addsrcrecordid>eNp9kUtv1DAUhSMEKkNhzwKEhSrEJoMfedhSN6NRW5BGAmlgbTmO3fHg2GnsVMO_x-mEEWXByvI937n32ifLXiO4RLAmn3onwpLBJUbpDnH1JFsgyFBeFQw-zRapVOe0wMXz7EUIewghKyk8y84oJgUu0SL7ueqM82AlTQu26m5UTirgNRDg2xhFNPcKbH1rxg6sd8I5ZcHVoR9UCKoFxoG4U-DGCBfB6uDdZJwq27sxtdt4a2498L2wKkjlwsvsmRY2qFfzeZ79uL76vv6cb77efFmvNrksKYt5WZWsVbSVDRJaS1lLqkWFadmwgmBZIFy2WDFaSNwyQnDDdMNqJXWNi6pBDTnPLo99-7HpVJtGx0FY3g-mE8Mv7oXhjxVndvzW3_OirmGV7B9m--DTh4TIO5P2t1Y45cfA6wpWkNI6ge__Afd-HFx6GscQYYrZAwSPkBx8CIPSpz0Q5FOGfMqQM8gx4g8ZJsvbv_c_GebQkn4x6yJIYfUgnDThhBGKy9Q3Ye9mbBrwR3086OP_Ca5Ha6M6xIS-OaL7EP1wYjGpCGEl-Q2hGsdj</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201282987</pqid></control><display><type>article</type><title>Amino Acid Sequence of a Putative Sodium Channel Expressed in the Giant Axon of the Squid Loligo opalescens</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Joshua J. C. Rosenthal ; Gilly, William F.</creator><creatorcontrib>Joshua J. C. Rosenthal ; Gilly, William F.</creatorcontrib><description>A full-length cDNA encoding a putative Na+channel (GFLN1) has been cloned from a library prepared from the stellate ganglion of Loligo opalescens. The cDNA encodes a predicted protein of 1784 amino acids. Regions of the GFLN1 protein with defined functional importance (membrane span S4, the SS1 and SS2 segments, and interdomain III-IV) are highly conserved among all vertebrate Na+channel α-subunit structures. Northern blot hybridization and RNase protection assays verify that mRNA corresponding to GFLN1 is expressed in neurons of the giant fiber lobe that form the giant axon. We propose that GFLN1 encodes the Na+channel that has been extensively studied in the squid axon.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.90.21.10026</identifier><identifier>PMID: 8234251</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Amino acids ; Animals ; Axons ; Axons - metabolism ; Base Sequence ; Biochemistry. Physiology. Immunology ; Biological and medical sciences ; Complementary DNA ; Conserved Sequence ; Decapodiformes - metabolism ; Deoxyribonucleic acid ; DNA ; DNA Primers ; DNA, Complementary ; Eels ; Electrophorus ; Fish ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Library ; Invertebrates ; Messenger RNA ; Molecular Sequence Data ; Mollusca ; Neurology ; Neurons ; Physiology. Development ; Polymerase Chain Reaction ; Rats ; RNA ; RNA, Antisense ; RNA, Messenger - biosynthesis ; RNA, Messenger - isolation & purification ; Sequence Homology, Amino Acid ; Sodium Channels - biosynthesis ; Sodium Channels - chemistry ; Squid ; Stellate Ganglion - metabolism ; Vertebrates</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1993-11, Vol.90 (21), p.10026-10030</ispartof><rights>Copyright 1993 The National Academy of Sciences of the United States of America</rights><rights>1994 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Nov 1, 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c589t-5659de8dcb1affcc7c8fa6285b9432c4125d2e984c2d9332b9fb97ecf7246b1b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/90/21.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2363395$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2363395$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,309,310,314,727,780,784,789,790,803,885,23930,23931,25140,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3825073$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8234251$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Joshua J. C. Rosenthal</creatorcontrib><creatorcontrib>Gilly, William F.</creatorcontrib><title>Amino Acid Sequence of a Putative Sodium Channel Expressed in the Giant Axon of the Squid Loligo opalescens</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A full-length cDNA encoding a putative Na+channel (GFLN1) has been cloned from a library prepared from the stellate ganglion of Loligo opalescens. The cDNA encodes a predicted protein of 1784 amino acids. Regions of the GFLN1 protein with defined functional importance (membrane span S4, the SS1 and SS2 segments, and interdomain III-IV) are highly conserved among all vertebrate Na+channel α-subunit structures. Northern blot hybridization and RNase protection assays verify that mRNA corresponding to GFLN1 is expressed in neurons of the giant fiber lobe that form the giant axon. We propose that GFLN1 encodes the Na+channel that has been extensively studied in the squid axon.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Axons</subject><subject>Axons - metabolism</subject><subject>Base Sequence</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>Complementary DNA</subject><subject>Conserved Sequence</subject><subject>Decapodiformes - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>DNA, Complementary</subject><subject>Eels</subject><subject>Electrophorus</subject><subject>Fish</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Library</subject><subject>Invertebrates</subject><subject>Messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>Mollusca</subject><subject>Neurology</subject><subject>Neurons</subject><subject>Physiology. Development</subject><subject>Polymerase Chain Reaction</subject><subject>Rats</subject><subject>RNA</subject><subject>RNA, Antisense</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - isolation & purification</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sodium Channels - biosynthesis</subject><subject>Sodium Channels - chemistry</subject><subject>Squid</subject><subject>Stellate Ganglion - metabolism</subject><subject>Vertebrates</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAUhSMEKkNhzwKEhSrEJoMfedhSN6NRW5BGAmlgbTmO3fHg2GnsVMO_x-mEEWXByvI937n32ifLXiO4RLAmn3onwpLBJUbpDnH1JFsgyFBeFQw-zRapVOe0wMXz7EUIewghKyk8y84oJgUu0SL7ueqM82AlTQu26m5UTirgNRDg2xhFNPcKbH1rxg6sd8I5ZcHVoR9UCKoFxoG4U-DGCBfB6uDdZJwq27sxtdt4a2498L2wKkjlwsvsmRY2qFfzeZ79uL76vv6cb77efFmvNrksKYt5WZWsVbSVDRJaS1lLqkWFadmwgmBZIFy2WDFaSNwyQnDDdMNqJXWNi6pBDTnPLo99-7HpVJtGx0FY3g-mE8Mv7oXhjxVndvzW3_OirmGV7B9m--DTh4TIO5P2t1Y45cfA6wpWkNI6ge__Afd-HFx6GscQYYrZAwSPkBx8CIPSpz0Q5FOGfMqQM8gx4g8ZJsvbv_c_GebQkn4x6yJIYfUgnDThhBGKy9Q3Ye9mbBrwR3086OP_Ca5Ha6M6xIS-OaL7EP1wYjGpCGEl-Q2hGsdj</recordid><startdate>19931101</startdate><enddate>19931101</enddate><creator>Joshua J. C. Rosenthal</creator><creator>Gilly, William F.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19931101</creationdate><title>Amino Acid Sequence of a Putative Sodium Channel Expressed in the Giant Axon of the Squid Loligo opalescens</title><author>Joshua J. C. Rosenthal ; Gilly, William F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c589t-5659de8dcb1affcc7c8fa6285b9432c4125d2e984c2d9332b9fb97ecf7246b1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Axons</topic><topic>Axons - metabolism</topic><topic>Base Sequence</topic><topic>Biochemistry. Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>Complementary DNA</topic><topic>Conserved Sequence</topic><topic>Decapodiformes - metabolism</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Primers</topic><topic>DNA, Complementary</topic><topic>Eels</topic><topic>Electrophorus</topic><topic>Fish</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Library</topic><topic>Invertebrates</topic><topic>Messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>Mollusca</topic><topic>Neurology</topic><topic>Neurons</topic><topic>Physiology. Development</topic><topic>Polymerase Chain Reaction</topic><topic>Rats</topic><topic>RNA</topic><topic>RNA, Antisense</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - isolation & purification</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sodium Channels - biosynthesis</topic><topic>Sodium Channels - chemistry</topic><topic>Squid</topic><topic>Stellate Ganglion - metabolism</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Joshua J. C. Rosenthal</creatorcontrib><creatorcontrib>Gilly, William F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Joshua J. C. Rosenthal</au><au>Gilly, William F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amino Acid Sequence of a Putative Sodium Channel Expressed in the Giant Axon of the Squid Loligo opalescens</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1993-11-01</date><risdate>1993</risdate><volume>90</volume><issue>21</issue><spage>10026</spage><epage>10030</epage><pages>10026-10030</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>A full-length cDNA encoding a putative Na+channel (GFLN1) has been cloned from a library prepared from the stellate ganglion of Loligo opalescens. The cDNA encodes a predicted protein of 1784 amino acids. Regions of the GFLN1 protein with defined functional importance (membrane span S4, the SS1 and SS2 segments, and interdomain III-IV) are highly conserved among all vertebrate Na+channel α-subunit structures. Northern blot hybridization and RNase protection assays verify that mRNA corresponding to GFLN1 is expressed in neurons of the giant fiber lobe that form the giant axon. We propose that GFLN1 encodes the Na+channel that has been extensively studied in the squid axon.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8234251</pmid><doi>10.1073/pnas.90.21.10026</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1993-11, Vol.90 (21), p.10026-10030 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_90_21_10026 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Amino acids Animals Axons Axons - metabolism Base Sequence Biochemistry. Physiology. Immunology Biological and medical sciences Complementary DNA Conserved Sequence Decapodiformes - metabolism Deoxyribonucleic acid DNA DNA Primers DNA, Complementary Eels Electrophorus Fish Fundamental and applied biological sciences. Psychology Gene Expression Gene Library Invertebrates Messenger RNA Molecular Sequence Data Mollusca Neurology Neurons Physiology. Development Polymerase Chain Reaction Rats RNA RNA, Antisense RNA, Messenger - biosynthesis RNA, Messenger - isolation & purification Sequence Homology, Amino Acid Sodium Channels - biosynthesis Sodium Channels - chemistry Squid Stellate Ganglion - metabolism Vertebrates |
| title | Amino Acid Sequence of a Putative Sodium Channel Expressed in the Giant Axon of the Squid Loligo opalescens |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T03%3A24%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Amino%20Acid%20Sequence%20of%20a%20Putative%20Sodium%20Channel%20Expressed%20in%20the%20Giant%20Axon%20of%20the%20Squid%20Loligo%20opalescens&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Joshua%20J.%20C.%20Rosenthal&rft.date=1993-11-01&rft.volume=90&rft.issue=21&rft.spage=10026&rft.epage=10030&rft.pages=10026-10030&rft.issn=0027-8424&rft.eissn=1091-6490&rft.coden=PNASA6&rft_id=info:doi/10.1073/pnas.90.21.10026&rft_dat=%3Cjstor_pnas_%3E2363395%3C/jstor_pnas_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201282987&rft_id=info:pmid/8234251&rft_jstor_id=2363395&rfr_iscdi=true |