1H-[13C] NMR Measurements of [4-13C]Glutamate Turnover in Human Brain
A limitation of previous methods for studying human brain glucose metabolism, such as positron emission tomography, is that metabolic steps beyond glucose uptake cannot be studied. Nuclear magnetic resonance (NMR) has the advantage of allowing the nondestructive measurement of13C distribution in spe...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1992-10, Vol.89 (20), p.9603-9606 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Rothman, D. L. Novotny, E. J. Shulman, G. I. Howseman, A. M. Petroff, O. A. C. Mason, G. Nixon, T. Hanstock, C. C. Prichard, J. W. Shulman, R. G. |
| description | A limitation of previous methods for studying human brain glucose metabolism, such as positron emission tomography, is that metabolic steps beyond glucose uptake cannot be studied. Nuclear magnetic resonance (NMR) has the advantage of allowing the nondestructive measurement of13C distribution in specific carbon positions of metabolites. In this study1H-[13C] NMR spectroscopy in conjunction with volume localization was used to measure the rate of incorporation of13C isotope from infused enriched [1-13C]glucose to human brain [4-13C]glutamate. In three studies C4 glutamate turnover time constants of 25, 20, and 17 min were measured in a 21-cm3volume centered in the region of the visual cortex. Based on an analysis of spectrometer sensitivity the spatial resolution of the method can be improved to |
| doi_str_mv | 10.1073/pnas.89.20.9603 |
| format | Article |
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L. ; Novotny, E. J. ; Shulman, G. I. ; Howseman, A. M. ; Petroff, O. A. C. ; Mason, G. ; Nixon, T. ; Hanstock, C. C. ; Prichard, J. W. ; Shulman, R. G.</creator><creatorcontrib>Rothman, D. L. ; Novotny, E. J. ; Shulman, G. I. ; Howseman, A. M. ; Petroff, O. A. C. ; Mason, G. ; Nixon, T. ; Hanstock, C. C. ; Prichard, J. W. ; Shulman, R. G.</creatorcontrib><description>A limitation of previous methods for studying human brain glucose metabolism, such as positron emission tomography, is that metabolic steps beyond glucose uptake cannot be studied. Nuclear magnetic resonance (NMR) has the advantage of allowing the nondestructive measurement of13C distribution in specific carbon positions of metabolites. In this study1H-[13C] NMR spectroscopy in conjunction with volume localization was used to measure the rate of incorporation of13C isotope from infused enriched [1-13C]glucose to human brain [4-13C]glutamate. In three studies C4 glutamate turnover time constants of 25, 20, and 17 min were measured in a 21-cm3volume centered in the region of the visual cortex. Based on an analysis of spectrometer sensitivity the spatial resolution of the method can be improved to <4 cm3. 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In conjunction with metabolic modeling and other NMR measurements this method can provide a measure of regional rates of the brain tricar-boxylic acid cycle and other metabolic pathways.</description><subject>Amino acid metabolism</subject><subject>Biochemistry</subject><subject>Biophysics</subject><subject>Blood plasma</subject><subject>Brain - metabolism</subject><subject>Carbon Isotopes</subject><subject>Glucose - metabolism</subject><subject>Glutamates - metabolism</subject><subject>Humans</subject><subject>Isotopes</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Metabolism</subject><subject>Nuclear magnetic resonance</subject><subject>Positron emission tomography</subject><subject>Spectroscopy</subject><subject>Time constants</subject><subject>Time Factors</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1P20AQhleoFQ0pZy5t5VM5Ocx-2OuVuNAIEiSgUkVPCK3Gzmxr5I-wayP499hKSuHCaQ7P886MXsYOOMw4aHm0bjDMMjMTMDMpyB024WB4nCoDH9gEQOg4U0J9Ynsh3AGASTLYZbtcgUm1mLBTvoxvuJzfRleXv6JLwtB7qqnpQtS66EbFI1tUfYc1dhRd975pH8hHZRMt-xqb6IfHsvnMPjqsAu1v55T9Pju9ni_ji5-L8_nJRVxIMDLOctRZnoBEXDkSPHdFnhtdpJg4hyYBVI60NBlAITAxoFaSgzCapKLUkZyy483edZ_XtCqGPz1Wdu3LGv2TbbG0b0lT_rV_2gebAM9giH_fxn1731PobF2GgqoKG2r7YLUUCrSRg3i0EQvfhuDJvZzgYMfe7di7zYwVYMfeh8TX15_99zdFD_zblo_Bf_TNgsN3Bev6qurosRvMLxvzLnStf1GFTEEpI58BV-ifSw</recordid><startdate>19921015</startdate><enddate>19921015</enddate><creator>Rothman, D. 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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino acid metabolism Biochemistry Biophysics Blood plasma Brain - metabolism Carbon Isotopes Glucose - metabolism Glutamates - metabolism Humans Isotopes Magnetic Resonance Spectroscopy Metabolism Nuclear magnetic resonance Positron emission tomography Spectroscopy Time constants Time Factors |
| title | 1H-[13C] NMR Measurements of [4-13C]Glutamate Turnover in Human Brain |
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